Horse bean root tip cell microkernel flaking method

Abstract

The present invention discloses a method for preparing the piece of root apiex microkernel of lima beans, the method comprises the steps of soaking seed to hasten germination to the seed of the lima beans, fixed-isolating the root tip, dyeing and performing and the like. The method integrates the fixing and the isolating to one step with a fixed-isolating liquid, lead to that the experiment whichneeds about 25 hours for completion formerly can be completed in about 30 minutes, the experiment time is greatly saved and the chemical consumption is reduced; and through the using of the cell nucleus dye liquor with simple operation and good dyeing effect the optimum microkernel piece-making effects of evident cell nucleus dyeing, large nuclearsubstance contrast ratio and definite cell profileare obtained thereby ensuring the feasibility for monitoring the environment with the microkernel technique of the lima beans root tip and the accuracy.

Description

technical field

[0001] The invention relates to a cell micronucleus preparation method, in particular to a faba bean root tip cell micronucleus preparation method. Background technique

[0002] Plant broad bean (Vicia faba L) root tip cells have large chromosomes, few in number, rich in DNA, and sensitive to mutagen. Broad bean root tip cell micronucleus technology was established by Francesca and MaTe-Hsiu in 1982, and it has been adopted by China, the United States, the United Nations Environment Program (UNEPA) and the World Health Organization (WHO) in environmental pollution monitoring and mutagen detection. It is recognized by many countries, regions and international organizations. In the "Water and Wastewater Monitoring and Analysis Methods" promulgated by the State Environmental Protection Administration of China in 2002, the broad bean root tip micronucleus test was listed as a B-type method used in the environmental monitoring and law enforcement process, and in ...