Paclitaxel genome rearrangement bacterial strain HDFS4-26 and breeding method of high-yield bacterial strain

Abstract

The invention relates to paclitaxel genome shuffling bacterial strain HDFS4-26 and a seed selection method of high yield paclitaxel bacterial strain, which belongs to the paclitaxel genome shuffling bacterial strain and the seed selection method of the paclitaxel engineering bacterial strain and solves the problem that the paclitaxel output of the bacterial strain which can be used in the paclitaxel microorganism fermentation at present is still relatively low. The paclitaxel genome shuffling bacterial strain HDFS4-26 is nodulisporium sylviforme and belongs to the nodulisporium and is preserved in the China Center for Type Culture Collection, the preservation date is February 28, 2008, the preservation number is CCTCC M 208026. The seed selection method of the high yield paclitaxel bacterial strain adopts firstly, the protoplast preparation; secondly, the inactivation of the protoplast; thirdly, the interfusion of the protoplast; fourthly, the regeneration of the protoplast; fifthly, the genome shuffling and sieving, therefore to obtain the high yield paclitaxel bacterial strain. The paclitaxel output of the bacterial strain HDFS4-26 is 516.37ug/L. The seed selection method of the high yield paclitaxel bacterial strain provided by the invention is simple, the effect is good, and the paclitaxel genome shuffling bacterial strain HDFS4-26 and the seed selection method of high yield paclitaxel bacterial strain lay the foundation of industrial production of bacterial strain providing high yield paclitaxel.

Description

technical field [0001] The invention relates to a paclitaxel genome rearrangement strain and a method for breeding paclitaxel engineering strains. Background technique [0002] Malignant tumors seriously threaten human health, and cancer is the second leading cause of death in human diseases after cardiovascular and cerebrovascular diseases. [0003] Paclitaxel is a diterpene alkaloid drug (molecular formula such as figure 1 shown). Because yew (also known as yew) is a rare tree species, its growth rate is slow, and its biological characteristics have high requirements on the ecological environment, and its natural renewal ability is poor, so the population is extremely limited. It is an endangered plant and its resources are very limited. Therefore, Scholars at home and abroad are actively researching and developing new drug sources of paclitaxel. [0004] Microbial fermentation production is the most ideal way to reduce costs and obtain paclitaxel in large quantities. ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the paclitaxel yield of the strains currently available for paclitaxel microbial fermentation is still low, and provide the paclitaxel genome rearrangement strain HDFS4-26 and the paclitaxel high-yielding strain breeding method

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