Mesenchyma stem cell suspension containing cell growth factor and uses thereof

A technology of mesenchymal stem cells and growth factors, applied in medical preparations containing active ingredients, liquid delivery, emulsion delivery, etc.

Inactive Publication Date: 2008-12-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have confirmed that epidermal growth factor and basic fibroblast growth factor play a vital role in promoting wound healing, but so far there is no evidence that the combin

Method used

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  • Mesenchyma stem cell suspension containing cell growth factor and uses thereof
  • Mesenchyma stem cell suspension containing cell growth factor and uses thereof
  • Mesenchyma stem cell suspension containing cell growth factor and uses thereof

Examples

Experimental program
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Embodiment 1

[0024] Example 1: Preparation of a suspension of mesenchymal stem cells containing cell growth factors

[0025] 1. Prepare cell suspension:

[0026] Isolation, culture and expansion of human bone marrow-derived mesenchymal stem cells or human early embryo-derived mesenchymal stem cells (see Zhou Dunhua, Huang Shaoliang, Wu Yanfeng, etc. for details. Research on the in vitro expansion and biological characteristics of human mesenchymal stem cells. Chinese Journal of Pediatrics, 2003, 41(8): 608-610.) prepared into a single mesenchymal stem cell suspension, press 2×10 6 Cells / ml were seeded in culture flasks, placed in a 37°C incubator and cultured with complete DMEM medium, and passaged and expanded when the cells reached 70%-80% confluence. The culture medium is DMEM containing 2mM glutamine, 100U / ml penicillin, 100μg / ml streptomycin and 10% fetal bovine serum (both products of Gibco, USA).

[0027] 2. Preparation of mesenchymal stem cell suspension containing human epiderma...

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PUM

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Abstract

The invention relates to the medical technical field of repairing wounds, which can be used for repairing wounds, particularly for healing deep burn wounds. The invention provides mesenchymal stem cell suspension which contains human epidermal growth factor and bovine basophilic fibroblast growth factor. The mesenchymal stem cell provides the sources for human marrows or embryo. When in use, the stem cell suspension is directly injected or smeared on wounds which have been removed necrotic tissues. Animal experiments show that the stem cell suspension can obviously repair deep burn wounds; besides, the new skin does not tend to generate blister or ulceration. The stem cell suspension of the invention is simple in preparation and conveniently for use, obviously repairing the deep burn wounds, thus effectively controlling the hyperplasia of anaphase keloid.

Description

technical field [0001] The invention relates to the technical field of medical wound repair, and is a mesenchymal stem cell suspension containing human epidermal growth factor and bovine basic fibroblast growth factor that can be used for wound repair, and is especially used for promoting the healing of deep burn wounds. technical background [0002] Deep burns include deep second-degree and third-degree burns. Deep second-degree burns are damages in the deep layer of the dermis. Deep second-degree wounds have residual dermis, and there are hair follicles, sweat glands, and sebaceous glands in the reticular layer. The epithelial proliferation of these skin appendages can form epithelial islands for wound repair, although skin grafting is not required. It can heal on its own, but scars are often left after healing, forming various contracture deformities. The longer the burn wound healing time, the more obvious the dysfunction and appearance abnormality caused by the healing...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K9/10A61P17/02
Inventor 夏照帆王钰唐洪泰肖仕初杨晓妍
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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