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Bolete extract, preparation and application thereof

A bolete, extract technology, applied in the directions of botanical equipment and methods, applications, chemicals used for biological control, etc., can solve problems such as no reports on the effect, achieve low cost, simple production method, promote effect of growth

Inactive Publication Date: 2009-01-14
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its role in plant disease control has not been reported

Method used

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  • Bolete extract, preparation and application thereof
  • Bolete extract, preparation and application thereof
  • Bolete extract, preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preparation of embodiment 1 black boletus extract

[0027] (1) Dry the commercially available black boletus at 40°C, weigh 10g, smash it with a tissue grinder, and pass through a 60-mesh sieve to obtain black boletus dry powder;

[0028] (2) Add 100ml of phosphate buffer solution (pH8.0, 25mmol / L) to the black boletus dry powder obtained in step (1) at 4°C, oscillate and mix well, and all the operations are carried out on ice to obtain a mixed solution;

[0029] (3) Centrifuge the mixture obtained in step (2) at 4° C. and 12,000 rpm for 20 minutes; discard the precipitate, and collect the supernatant to obtain 90 ml of Boletus nigella extract, which should be frozen at -20° C. for future use.

[0030] The protein concentration of the black boletus extract was detected with the BCA protein concentration kit (Pierce company, U.S.), and the assay method was carried out according to the instructions of the kit. As a result, the protein concentration of the above-mention...

Embodiment 2

[0031] The preparation of embodiment 2 yellow boletus extract

[0032] (1) Dry the commercially available Boletus cerevisiae at 40°C, weigh 10g, crush it with a tissue grinder, and pass through a 60-mesh sieve to obtain a dry powder;

[0033] (2) Add 100ml of phosphate buffer (PH8.0, 25mmol / L) to the boletus dry powder obtained in step (1) at 4°C, shake and mix, and operate on ice to obtain a mixed solution;

[0034] (3) Centrifuge the mixture obtained in step (2) at 4°C and 12000rpm for 20min; discard the precipitate, and collect the supernatant to obtain 90ml of Boletus flavus extract, which should be frozen at -20°C for future use.

[0035] The BCA protein concentration kit (Pierce, USA) was used to detect the protein concentration of the Boletus flavus extract, and the assay method was carried out according to the instructions of the kit. As a result, the protein concentration of the Boletus flavus extract was 7.3425mg / ml.

Embodiment 3

[0036] Example 3 Antiviral Activity Test of Boletus nigra Extract to Tobacco Mosaic Virus

[0037] (1) Processing: the black boletus extract prepared in Example 1 is diluted with water successively to three concentrations of protein concentrations of 10.00 μg / ml, 20.00 μg / ml, and 40.00 μg / ml, and compared with water;

[0038] (2) When the leaves of the same seedling age are grown to 8-10 true leaves, the topping treatment is carried out, and the 5th to 8th true leaves are reserved, and the whole plant of the dead-spotted Sansheng tobacco is sprayed. Do three replicates, three plants per replicate, three leaves per plant;

[0039] (3) After 72 hours of induction, remove the main veins from the tobacco leaves of common tobacco infected with tobacco mosaic virus, mix with 0.01M phosphate buffer solution of pH 7.0 according to the weight-to-volume ratio of 1:20, add 0.015g corundum per 20ml, and Grind the leaves, inoculate the induced tobacco with dry spot by rubbing, rinse with ...

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Abstract

The invention discloses bolete extract and a preparation method thereof; the method mainly comprises the following steps: bolete is dried under 30 DEG C to 40 DEG C, crushed, sieved by a 60 mesh to 500 mesh sieve, extracted by phosphate buffer under 0 DEG C to 4 DEG C and centrifugated for 20min to 30min under 0 DEG C to 4 DEG C at 10000rpm to 12000rpm; supernatant fluid is collected and the bolete extract is obtained. The invention provides an effective method for preventing tobacco mosaic virus; the inhibition rate of the bolete extract to the tobacco mosaic virus reaches 67.49 percent to 92.51 percent; the bolete extract belongs to natural extract, has no harm to the human beings, is environment friendly; the production method of the invention is simple, and the cost is low.

Description

technical field [0001] The invention belongs to biological extracts, and in particular relates to a boletus extract, a preparation method thereof, and an application of the extract in preventing and treating plant diseases. Background technique [0002] Plant virus diseases are plant diseases second only to fungal diseases, causing serious harm to agricultural production. It is estimated that the annual losses caused by plant virus diseases in the whole world exceed 15 billion U.S. dollars, and the damage caused by tobacco mosaic virus (Tobacco mosaic virus) reaches more than 100 million U.S. dollars every year (Wu Yunfeng. World Agriculture, 1995, (5 ): 35~36). At present, there is no effective control method for tobacco mosaic virus disease. Therefore, searching for safe and antiviral active biologically derived antiviral active substances or disease-resistant genes is currently a research hotspot in the field of plant virus disease prevention and control. [0003] In t...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01P3/00
Inventor 严红李兴红赵素芳周莹黄金宝林秀敏
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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