Method for preparing Bacillus Subtilis fermented mixed feed for birds

A Bacillus subtilis, mixed feed technology, applied in the direction of animal feed, animal feed, additional food elements, etc., can solve problems such as spread and human harm, and achieve the effects of simple production process, growth promotion, and nutritional composition improvement.

Inactive Publication Date: 2009-01-21
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] For the feed used in poultry breeding, on the one hand, it is necessary to consider the nutrients in the feed to improve the cost performance of the feed, and on the other hand, it is also necessary to consider whether the feed itself can improve the resistance of the poultry to pathogenic microorganisms. Ordinary feeds are often added with "three elements". That is: antibiotics, hormones and vitamins, but the abuse of "three elements" will affect the quality of poultry meat and eggs as human food, causing potential harm to humans. Therefore, there is an urgent need for a nutrition that can improve roughage. Composition, so that the plant fiber can be transformed into a feed that is easily absorbed by poultry with small molecules and can improve the resistance of poultry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method for poultry bacillus subtilis fermented mixed feed, comprising the steps of:

[0023] (1) Use broth culture medium as seed medium, cultivate Bacillus subtilis under 40 DEG C of aerobic conditions for 18 hours, and become first-class seeds;

[0024] (2) inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt the broth medium as the secondary seed medium, and cultivate it for 18 hours under aerobic conditions at 40° C. to become secondary seeds;

[0025] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, using broth medium as the fermentation medium, under 40 ° C aerobic conditions, adopt timing or continuous fed-batch fermentation for 18 hours, and become tertiary seeds;

[0026] (4) After mixing the rice bran, bran and crushed corn with a mass ratio of 1:1:1, they are evenly mixed with the third-grade seed liquid, and the mass ratio of the rice bran, bran and crushed corn mixed with the t...

Embodiment 2

[0029] A preparation method for poultry bacillus subtilis fermented mixed feed, characterized in comprising the following steps:

[0030] (1) Use broth medium as seed medium, cultivate Bacillus subtilis under 30 DEG C of aerobic conditions for 30 hours, and become first-class seeds;

[0031] (2) Inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt the broth medium as the secondary seed medium, and cultivate it under aerobic conditions at 30° C. for 30 hours to become secondary seeds;

[0032] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, using broth medium as the fermentation medium, and under 30°C aerobic conditions, adopt timing or continuous fed-batch fermentation for 36 hours to become tertiary seeds;

[0033] (4) After mixing rice bran, bran and crushed corn with a mass ratio of 1:5:1, they are uniformly mixed with the third-grade seed liquid, and the mass ratio of the rice bran, bran and crushed corn m...

Embodiment 3

[0036] A preparation method for poultry bacillus subtilis fermented mixed feed, characterized in comprising the following steps:

[0037] (1) Use broth medium as seed medium, cultivate Bacillus subtilis under aerobic conditions at 45°C for 8 hours to become first-class seeds;

[0038] (2) Inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt the broth medium as the secondary seed medium, and cultivate it under 45° C. aerobic conditions for 8 hours to become secondary seeds;

[0039] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, using broth medium as the fermentation medium, under 45 ° C aerobic conditions, adopt timing or continuous fed-batch fermentation for 8 hours, and become tertiary seeds;

[0040] (4) After mixing rice bran, bran and crushed corn with a mass ratio of 1:1 to 5:1 to 5, they are evenly mixed with the third-grade seed liquid, and the rice bran, bran and crushed corn are mixed with the third...

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PUM

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Abstract

The invention discloses a preparation method of a bacillus subtilis fermented mixed feed for poultry. The method comprises the following steps: (1) the bacillus subtilis is cultivated to become a primary seed by broth culture; (2) the primary seed liquid is in amplification culture by the broth culture to become a secondary seed; (3) the secondary seed liquid is in fermentation culture by the broth culture to become a tertiary seed; (4) rice bran, wheat bran and crushed corn are mixed, and then are evenly mixed with the tertiary seed liquid, the mixture is further fermented and cultivated to obtain a first fermented feed; (5) the first fermented feed is mixed with bean cakes, the wheat bran, fish meal, bone meal, conch powder and common salt to obtain the bacillus subtilis fermented mixed feed for the poultry. The method of the invention has the advantages of improving the nutritional components of a coarse feed and causing the vegetable fiber to be transformed into small molecules which are easy to be absorbed by the poultry. By applying the mixed feed prepared by the method to the poultry, the mixed feed can promote the growth of the poultry without using antibiotics and hormone.

Description

technical field [0001] The invention relates to a preparation method of microbial fermented mixed feed, in particular to a preparation method of poultry Bacillus subtilis fermented mixed feed. Background technique [0002] For the feed used in poultry breeding, on the one hand, it is necessary to consider the nutrients in the feed to improve the cost performance of the feed, and on the other hand, it is also necessary to consider whether the feed itself can improve the resistance of the poultry to pathogenic microorganisms. Ordinary feeds are often added with "three elements". That is: antibiotics, hormones and vitamins, but the abuse of "three elements" will affect the quality of poultry meat and eggs as human food, causing potential harm to humans. Therefore, there is an urgent need for a nutrition that can improve roughage. Composition, so that the plant fiber can be transformed into a feed that is easily absorbed by poultry with small molecules and can improve the resist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16A23K10/12A23K10/22A23K10/26A23K10/37A23K20/22
CPCY02P60/87
Inventor 田杏芳王连民
Owner TIANJIN SHENGJI GRP CO LTD
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