Method for preparing micro organism cellulose

A technology of microbial cellulose and bacterial cellulose, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of application limitations, high production costs, and expensive prices

Inactive Publication Date: 2009-02-04
HEILONGJIANG PROV LIGHT INDAL SCI RES ACAD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Since microbial cellulose has been commercially applied in industries such as audio, food, medicine, and paper, but the current produ

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  • Method for preparing micro organism cellulose

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Embodiment Construction

[0015] The present invention will be described in more detail below in conjunction with specific embodiments:

[0016] 1.1. Source of fermentation strains

[0017] In this example, Acetobacter xylinum was purchased from China Industrial Microbial Culture Collection and Management Center,

[0018] 1.2 Strain mutagenesis

[0019] Firstly, Acetobacter xylinum was made into a bacterial suspension, and a 15W ultraviolet lamp was used at a distance of 30cm and irradiated for 20 seconds. Stirring and irradiating to make the bacteria uniformly absorb ultraviolet light waves. The above irradiation process is carried out in a dark room to avoid photorepair. The bacteria after UV mutagenesis were transferred to a sterile test tube and immediately immersed in ice water for 1 hour. Under low temperature conditions, the activities of various enzymes involved in the repair of the mutant were inhibited, making the repair difficult. Helps increase the mutation rate. Use the sandwich method to incu...

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Abstract

The invention provides a preparation method for microbe cellulose. Firstly, fermentation medium is prepared: 5 percent of corn hydrolysis sugar, 0.5 percent of ammonium sulfate, 0.5 percent of NaCl, 0.2 percent of magnesium sulfate, 0.1 percent of acetic acid and 0.15 percent of lactate are sterilized under the temperature of 110 to 150 DEG C for 10 to 30 minutes and cooled under the temperature of 30 DEG C, are inoculated with 4 percent of ultraviolet to mutagenize MC-5 Acetobacter xylinum seed liquid, and are cultured by a shaking table for 120 hours; then the fermentation liquid is centrifugated in a high speed of 3000r/min for 20 minutes in a high-speed centrifuger, supernatant fluid is poured away, centrifuged sentiments are taken and heated in boiling water bath with the temperature of 100 DEG C for 30 minutes by 4 percent NaOH solution to remove mycoprotein and then are centrifuged; sediments are taken and added with neutral detergent solution to be heated in 100 DEG C water bath for one hour to remove the impurities such as saccharide, lipid and the like in the sentiments, then are repeatedly washed by de-ionized water and 0.5 percent acetic acid, and finally are dried under the temperature of 80 DEG C for 24 hours to obtain bacterial cellulose.

Description

(1) Technical field [0001] The invention relates to a method for preparing microbial cellulose. (2) Background technology [0002] Cellulose is the most productive natural polymer on earth. Its output reaches 100 billion tons per year. It is widely found in various advanced plants, as well as lower plants, bacteria and individual lower animals, such as those grown in the ocean. Cellulose is also contained in green algae and some marine lower animals, and its existence form is composed of a three-dimensional structure with hemicellulose and lignin. Microbial cellulose is different from the cellulose that exists widely in nature. It exists in the form of pure cellulose and has the following characteristics: 1. The cellulose has high purity, high crystallinity and high degree of overlap, which is very beneficial in the preparation of microfiber products. . 2. The fiber diameter of microbial cellulose is between 0.01-0.1μm, the elasticity is several to ten times that of ordinary fibe...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02
Inventor 陈成张超吕伟民吴镝常洪娟李娜安晓光
Owner HEILONGJIANG PROV LIGHT INDAL SCI RES ACAD
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