Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin

A technique for thyroglobulin and chemiluminescence immunoassay, which is applied in the field of medical immunoassay detection and analysis, can solve the problems of complicated operation steps, long operation time period and long operation time, and achieves the effect of simplifying operation steps, saving operation time and improving sensitivity

Inactive Publication Date: 2009-03-04
北京科美东雅生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The current methods for measuring Tg include direct competition solid-phase radioimmunoassay (RIA), double antibody sandwich immunoradioassay (IMRA) or enzyme-linked immunoassay (ELISA), which are all affected by the body's own thyroglobulin autoantibodies. Interference, and the radioimmunoassay method has problems such as radioactive hazards to operators, waste disposal, short product shelf life, and long operation time; the enzyme-linked immunoassay method has low sensitivity, cumbersome operation steps, and long operation time cycle, which is easy to cause Issues such as false positive or false negative results

Method used

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  • Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin
  • Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin
  • Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin

Examples

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Embodiment 1

[0023] Example 1 Preparation of thyroglobulin chemiluminescence immunoassay assay kit of the present invention

[0024] 1. Preparation of enzyme-labeled antibody and selection of enzyme-labeled antibody concentration

[0025] 1. Thyroglobulin antibody was coupled with horseradish peroxidase by glutaraldehyde oxidation method, fully dialyzed with PBS, added an equal amount of high-quality glycerin, and stored below -20°C. specific:

[0026] 1) Dissolve 10 mg of HRP in 0.2 mL of 0.1 mol / L pH 6.8 PBS containing 1.25% glutaraldehyde, place at room temperature for 18 hours, and fully dialyze to remove excess glutaraldehyde; 2) Add normal saline to 1 mL, then add 5 mg Mix the purified antibody globulin and 0.1 mL of 1 mol / L carbonate buffer solution of pH 9.6, and place in a refrigerator at 4°C for 24 hours; 3) Add 0.1 mL of 0.2 mol / L lysine solution, and place at room temperature for 2 hours; 4 ) was fully dialyzed with PH 7.2, 0.15mol / L PBS, the precipitate was removed by centri...

Embodiment 2

[0063] Example 2 Preparation of Thyroglobulin Chemiluminescent Immunoassay Kit of the Present Invention

[0064] Thyroglobulin chemiluminescent immunoassay assay kits were prepared in the same manner as in Example 1 except that plastic beads were used as carriers.

Embodiment 3

[0065] Example 3 Preparation of Thyroglobulin Chemiluminescent Immunoassay Kit of the Present Invention

[0066] The thyroglobulin chemiluminescent immunoassay assay kit was prepared in the same manner as in Example 1 except that the plastic tube was used as the carrier.

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Abstract

The invention relates to a kit which uses enzyme to catalyze a chemiluminescent substrate, the absorbance of a chromogenic substrate in enzyme immunoassay is replaced by a light signal which is generated through detecting the chemiluminescent substrate and has the same absorbance as the chromogenic substrate, the sensitivity of the detection light signal is higher than the absorbance of the detection chromogenic substrate and the detection light signal provides specific, rapid and reliable basis for the diagnosis of thyroglobulin. The invention provides a kit for conveniently, rapidly, sensitively and stably detecting thyroglobulin and a preparation method thereof.

Description

technical field [0001] The invention belongs to the technical field of medical immune detection and analysis, and specifically provides a human thyroglobulin enzymatic chemiluminescence immunoassay kit, which is suitable for the enzymatic chemiluminescence detection and analysis of thyroglobulin. Background technique [0002] Thyroglobulin (Tg) is a glycoprotein with a molecular weight of about 660kD, which acts as a prohormone in the synthesis of major thyroid hormones such as thyroxine and triiodothyronine. In thyroid tumors, subacute thyroiditis, Hashimoto's thyroiditis, and Graves' disease, the number of Tg will be increased; measuring the concentration of Tg can help in the treatment of the symptoms of congenital hypothyroidism in infants. [0003] The current methods for measuring Tg include direct competitive solid-phase radioimmunoassay (RIA), double antibody sandwich immunoradiometric assay (IMRA) or enzyme-linked immunoassay (ELISA), which are all affected by the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/545G01N21/76
Inventor 靳辉应希堂宋胜利胡国茂郑金来于尚永
Owner 北京科美东雅生物技术有限公司
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