Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin
A technique for thyroglobulin and chemiluminescence immunoassay, which is applied in the field of medical immunoassay detection and analysis, can solve the problems of complicated operation steps, long operation time period and long operation time, and achieves the effect of simplifying operation steps, saving operation time and improving sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Example 1 Preparation of thyroglobulin chemiluminescence immunoassay assay kit of the present invention
[0024] 1. Preparation of enzyme-labeled antibody and selection of enzyme-labeled antibody concentration
[0025] 1. Thyroglobulin antibody was coupled with horseradish peroxidase by glutaraldehyde oxidation method, fully dialyzed with PBS, added an equal amount of high-quality glycerin, and stored below -20°C. specific:
[0026] 1) Dissolve 10 mg of HRP in 0.2 mL of 0.1 mol / L pH 6.8 PBS containing 1.25% glutaraldehyde, place at room temperature for 18 hours, and fully dialyze to remove excess glutaraldehyde; 2) Add normal saline to 1 mL, then add 5 mg Mix the purified antibody globulin and 0.1 mL of 1 mol / L carbonate buffer solution of pH 9.6, and place in a refrigerator at 4°C for 24 hours; 3) Add 0.1 mL of 0.2 mol / L lysine solution, and place at room temperature for 2 hours; 4 ) was fully dialyzed with PH 7.2, 0.15mol / L PBS, the precipitate was removed by centri...
Embodiment 2
[0063] Example 2 Preparation of Thyroglobulin Chemiluminescent Immunoassay Kit of the Present Invention
[0064] Thyroglobulin chemiluminescent immunoassay assay kits were prepared in the same manner as in Example 1 except that plastic beads were used as carriers.
Embodiment 3
[0065] Example 3 Preparation of Thyroglobulin Chemiluminescent Immunoassay Kit of the Present Invention
[0066] The thyroglobulin chemiluminescent immunoassay assay kit was prepared in the same manner as in Example 1 except that the plastic tube was used as the carrier.
PUM
Property | Measurement | Unit |
---|---|---|
Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com