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Multi-layer diaphragm structure perfusion bioreactor and application

A bioreactor and sheet structure technology, applied in the field of biomedicine, can solve the problems of uneven distribution of liver cells, unfavorable and sufficient material exchange, etc., and achieve the effects of compact structure, convenient transportation and reasonable design.

Active Publication Date: 2009-03-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the distribution of liver cells in the hollow fiber tube is not uniform, and the semi-permeable membrane barrier or collagen layer is not conducive to sufficient material exchange between the cultured cells and the patient's blood.

Method used

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  • Multi-layer diaphragm structure perfusion bioreactor and application
  • Multi-layer diaphragm structure perfusion bioreactor and application
  • Multi-layer diaphragm structure perfusion bioreactor and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] see Figure 1-4 , a perfusion bioreactor with a multi-layer membrane structure for artificial liver, the reactor is composed of a cylindrical box 1, a liquid outlet 2, a liquid inlet 3, a concentric circle membrane 4 and a solid circle membrane 5, the box 1 The inner wall is provided with a groove 6, and the concentric circular diaphragm 4 is provided with a channel 7, and the channel 7 is a channel for liquid to flow through. The edge of the solid circular diaphragm 5 has a grid-shaped hole 8, which is convenient for liquid to overflow, and the concentric The circular diaphragm 4 and the solid circular diaphragm 5 are fitted into the box body 1 through the groove 6 to form a sufficient material exchange environment of a multi-layer film structure. The liquid inlet 3 and the liquid outlet 2 are respectively connected to the screw cap 9 and the screw cap 10, Connected by threads, the screw cap 9 and the screw cap 10 are made of 600-mesh cell mesh material.

[0018] The ...

Embodiment 2

[0020] Referring to Fig. 5, the use process is as follows: the blood 11 flows out, and is separated into cell components and plasma components through the plasma separator 12, wherein the plasma components enter the extracorporeal circulation pool 13, then enter the reactor 14 from the liquid inlet 3, pass through the solid circular diaphragm 5 edge mesh hole 8 overflows, and then continues to overflow from the central channel 7 of the concentric circular diaphragm 4, the overall flow of plasma is S-shaped, and the hepatocytes grown on the concentric circular diaphragm 4 and the solid circle 5 are in the flow process Direct contact (hepatocytes are pre-inoculated, see below for specific steps), after sufficient substance exchange, the liquid flows out through the liquid outlet 2 and returns to the circulation pool 13, and after multiple cycles, it is reinfused into the body through the pipeline 15 together with the cell components.

[0021] Exogenous hepatocytes can be selected...

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Abstract

The invention provides a perfusion type bioreactor with a multilayer membrane structure, which comprises a cylindrical case body, a liquid inlet, a liquid outlet, a concentric membrane and a solid circular membrane, wherein a groove is arranged on the inner wall of the case body; a channel is arranged in the middle of the concentric membrane; a latticed hole is arranged on the edge of the solid circular membrane; the membranes are embedded in the case body through the groove; and the liquid inlet and the liquid outlet are in threaded connection with a spiral cover. The perfusion type bioreactor provides a three-dimensional growing environment for cells, and allows the cells to be distributed evenly, so that the cells can maintain active function for a long time; flood flows through the multilayer membrane structure in the reactor and is directly contacted with the cells for sufficient exchange of substances. The perfusion type bioreactor has the advantages of reasonable design, compact arrangement of the structure, easy amplification and convenient transportation, and can be applied to a biologic artificial liver hepatic perfusion system.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a medical device, which is a core device for treating a biological or hybrid artificial liver—a perfusion bioreactor with a multilayer diaphragm structure for an artificial liver. Background technique [0002] Bioartificial liver is generally composed of cell components and extracorporeal circulation device (bioreactor), which can temporarily replace the liver function of severe disease, so that patients can successfully transition to liver transplantation or recover spontaneously. It is a necessary supplement for liver transplantation treatment. Applications in the treatment of failure are promising. At present, research centers in many countries in the world have conducted in-depth research on cells and bioreactors, and some bioartificial liver systems have entered the clinical verification stage, but the effect is not satisfactory, and there is no established bioartificial l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 李兰娟吕国良喻成波章益民潘小平徐威
Owner ZHEJIANG UNIV
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