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Method for preparing small-sized mammalian skin rolled and avulsed wound model

A technology of mammalian avulsion injury, which is applied in the field of rat skin rolling avulsion injury model, which can solve the problems of difficult accurate control of avulsion skin flap necrosis area, inconvenient specimen research, high experimental cost, etc., to achieve skin flap necrosis Constant area, easy operation and good repeatability

Inactive Publication Date: 2009-03-18
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to accurately control the necrotic area of ​​the avulsion flap formed by the pig hindlimb skin avulsion injury model, and the cost of various experiments is quite high, making it difficult to carry out large-scale specimen research

Method used

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  • Method for preparing small-sized mammalian skin rolled and avulsed wound model
  • Method for preparing small-sized mammalian skin rolled and avulsed wound model
  • Method for preparing small-sized mammalian skin rolled and avulsed wound model

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0022] Experimental Materials

[0023] 18 8-week-old Sprague-Dawley rats (provided by the Experimental Animal Center of Fourth Military Medical University) were selected and divided into three groups, 6 in each group, male or female, weighing 280-320 g, and fed normally. OLYMPUS CX31 microscope and camera system (Japan), LEICA-2135 rotary microtome (Germany), paraffin embedding microtome (Shanghai).

[0024] experiment apparatus

[0025] Self-made skin avulsion wound forming device, as shown in Figure 1, comprises base plate 2, support 4, and support 4 both sides are symmetrically provided with lead screw 5 that links to each other with base plate, support is a rectangular frame structure, and lead screw 5 stretches out rectangular frame Nuts 11 are provided at the upper and lower crossbeams, and the leading screw 5 and the nuts 11 form a he...

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Abstract

The invention discloses a method for preparing the models of grinding-pressing-tearing-peeling wound on the skin of small mammals, which comprises that: (1) after depilation and antisepsis are carried out on the skin on the back of a rat, back skin peeling surgery is carried out for forming a rectangular skin flap; (2) the prepared rectangular skin flap on the back of the rat is placed on the skin grinding-pressing-tearing-peeling shaping machine, uniform rotation and transversal movement of the muller are controlled electrically, when the rubber gear at the edge of the muller contacts the skin flap, grinding-pressing-tearing-peeling is produced, the pressure on the skin flap acted by the muller is controlled at 1.0-3.5kg, and the grinding-pressing-tearing-peeling wound of the rat is formed; (3) after seven days, methylene blue is used to indicate the scope of survival and putrescence of the skin flap, statistical analysis on the survival rate of the skin flag is carried out; (4) the model of grinding-pressing-tearing-peeling wound is established, that is the grinding pressure is 2.0-2.5kg, the area of necrotic tissue is moderate and accounts for 1 / 3 of the total area of the skin flap. The method of the invention has the advantages of accurate control on the produced grinding pressure, simple operation, invariable necrotic area of the skin flaps and good repetition, and the model of the invention is a better model for researching the grinding-pressing-tearing-peeling wound mechanism.

Description

technical field [0001] The invention relates to a method for preparing a small mammal skin rolling avulsion injury model, in particular to a method for preparing a rat skin rolling avulsion injury model. Background technique [0002] Establishing a small mammalian skin crush avulsion model is an extremely important experimental method and means for studying skin avulsion wound repair and medication. Skin avulsion is one of the common traumas in plastic surgery, and its incidence has been increasing in recent years. After skin avulsion injury, the avulsion tissue often has blood circulation and maintains a certain vitality in the early stage, but with the passage of time after injury, the injured tissue gradually appears secondary necrosis. In the past, there were few basic researches on skin avulsion injury, mainly due to the lack of animal models of skin avulsion injury. [0003] A practical skin crush avulsion model is a prerequisite for the study of drugs and treatments...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61K49/00A61D1/00
Inventor 潘宝华夏炜胡强刘宾郭树忠
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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