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Sargassum thunbergii young seedling large-scale breeding method using leaves tissue culture method

A tissue culture, Salina sp. technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve problems such as lagging development of marine biotechnology, and achieve easy control of culture conditions, less breeding space, and less plant material. Effect

Inactive Publication Date: 2011-05-04
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of marine biotechnology is relatively lagging behind, and few studies have used tissue culture to propagate economical algae seedlings
There is no relevant report on the method of tissue culture of Sargassum leaves to breed seedlings

Method used

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  • Sargassum thunbergii young seedling large-scale breeding method using leaves tissue culture method
  • Sargassum thunbergii young seedling large-scale breeding method using leaves tissue culture method

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Experimental program
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Effect test

Embodiment

[0049] This experiment started on June 24, 2005 and ended on April 11, 2006. Sargassum samples were collected from the intertidal zone of Qingdao Second Beach (35.35°N, 119.30°E, 10-35cm below the water surface) on June 24, 2005. The sample cleaning, holding and operation process are the same as the above-mentioned experimental operation. The culture medium adopts general-purpose ES seawater culture medium and adds trace elements (according to the ingredients of BG-11 formula). The culture temperature was 17°C. The leaf regeneration rate was 33%. The light intensity is 35μmol / m 2 per s. The photoperiod is 9:15h (light:dark). Tissue culture management is the same as above. The regeneration process of leaves see figure 2 .

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Abstract

The invention relates to the propagation of Sargassum thunbergii, in particular to a method for massively propagating the seedlings of the Sargassum thunbergii by a tissue culture vane method, comprising the steps of: 1) picking the complete principal branches in the sample of the Sargassum thunbergii and using disinfected seawater to disinfect; 2) then using AgCl to disinfect and process; using a bistoury to take down the wide and large vanes in the middle lower part of the principal branches and using a nipper to arrange the wide and large vanes into prepared culture liquid; the culturing temperature is between 13 and 20 DEG C; the illumination intensity is 15 to 50 [mu]mol / m<2> per s; and the illumination: darkness in the illumination period is equal to 8 to 10: 16 to 14h; 3) needing not to ventilate 7 to 10 days before culturing; after the 7 to 10 days, beginning to ventilate and culture after the wound healing of the vanes is finished; changing the culture liquid every 35 to 45 days; after culturing for 3 months, a plurality of buds are generated in the middle lower parts of the vanes; and after culturing for 5 months, regenerated strains can be collected to carry out fixed planting and culturing. The method needs less plant materials and less culturing space, can carry out idioplasm preservation for a long period, and has large fecundity and pure seedling quality, and the like.

Description

technical field [0001] The invention relates to the breeding of sargassum, in particular to a method for mass breeding sargassum seedlings by tissue culture leaf method. Background technique [0002] Sargassum thunbergii (Mert.) O. Kuntze belongs to Phaeophyta, Aspophyta, Sargassumaceae, and Sargassum genus. It is a warm temperate seaweed commonly found in coastal areas of my country. It grows in mid-tidal and low-tidal rocks or in water, land or bogs in high and mid-tidal zones. Some plants can be exposed to sunlight for a long time without affecting their growth and development. Sargassum not only occupies an important position in the marine ecosystem, but also has important application value in industries such as aquaculture, medicine, health care and chemical industry. Therefore, sargassum has very broad market development prospects [0003] Sargassum is an important bait for sea cucumber and abalone. In the juvenile ginseng breeding period, in addition to continuing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 叶乃好方建光邹健蒋增杰毛玉泽
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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