Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio

The technology of leech vibrio and mutagenic strain is applied in the application field of ultraviolet-induced mutation in enhancing the lysis performance of leech vibrio, can solve the problems of long time, difficult recovery of mutation frequency, difficult screening and the like, and achieves simple method and convenient operation. , Improve the effect of cracking capacity

Inactive Publication Date: 2012-03-28
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

Therefore, at present, scholars at home and abroad mostly use the method of natural screening, which not only takes a long time, but also is difficult to obtain.
Ultraviolet mutagenesis is still one of the most commonly used and effective methods in microbial breeding so far. It has the advantages of high mutation frequency and is not easy to recover. no reports yet

Method used

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  • Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio
  • Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio
  • Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The application of UV-induced mutagenesis, supplemented by infrared light source and LiCl-enhanced mutagenesis includes the following specific steps and conditions:

[0028] 1. Stabilized light waves

[0029] In the dark room, place a 15W ultraviolet lamp above the operating table, and place an infrared lamp with a power of 20W as an auxiliary infrared light source on both sides of the operating table, and turn on the ultraviolet light source 30 minutes in advance to stabilize the light wave;

[0030] 2. Sterile Induced Mutagenesis

[0031] 100mL of Bdellovibrio wild strain Bdellovibrio sp.BDJ01 culture solution, which has been preserved in the Chinese Type Culture Collection Center of Wuhan University in Wuhan City, China, with the preservation number CCTCC NO: M 208011, was kept at 4°C and at a speed of 5000×g. Centrifuge for 20 minutes, take the centrifuged supernatant and centrifuge at 12000×g for 20 minutes at 4°C, and suspend the precipitate with 10 mL of DNB liq...

Embodiment 2

[0051] The application of UV-induced mutagenesis, supplemented by infrared light source and LiCl-enhanced mutagenesis includes the following specific steps and conditions:

[0052] 1. Stabilized light waves

[0053] In the dark room, place a 20W ultraviolet lamp above the operating table, and place 25W infrared lamps as auxiliary infrared light sources on both sides of the operating table. Turn on the ultraviolet light source 60 minutes in advance to stabilize the light wave.

[0054] 2. Sterile Induced Mutagenesis

[0055] The concentrating method of Bdellovibrio wild strain BDJ01 is the same as Example 1, and the obtained concentration is 2×10 7 Pfu / mL Bdellovibrio wild strain concentrate; under the state of filtering visible light, the above 2×10 7 Add 5 mL of pfu / mL concentrated solution into a 9 cm sterile petri dish, then add 1.67 mL of LiCl solution with a concentration of 1.0% to the petri dish to make the final concentration reach 0.25%, add a sterile magnetic rotor...

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Abstract

The invention belongs to the technical field of mutation breeding of microorganisms, and particularly relates to application of ultraviolet-induced mutation in the strengthening pyrolysis performance of bdellovibrio bacteria. The application takes the ultraviolet-induced mutation as a main way and is assisted with an infrared light source and LiCl enhanced mutagenesis to perform induced mutation under the slow stirring, the time is between 2 and 8 minutes, and the radiation distance is between 5 and 20 centimeters; and the bdellovibrio bacteria is preserved under dark light and ice bath conditions and is accumulatively mutagenized until the requirement is achieved. The application has a simple method and convenient operation, has remarkable effect on strengthening the pyrolysis performance of the bdellovibrio bacteria, can pyrolyze two salmonella strains which fail to be pyrolyzed originally and a plurality of Gram-positive bacteria (including bacillus aerogenes capsulatus, clostridium difficile, streptococcus suis and enterococcus) proven by the experiment, solve the problems that usually the bdellovibrio bacteria can not pyrolyze the Gram-positive bacteria and the pyrolysis ability is extremely weak even if the bdellovibrio bacteria can pyrolyze the Gram-positive bacteria, and widen the application range of the bdellovibrio bacteria.

Description

technical field [0001] The invention belongs to the technical field of microbial mutation breeding, and in particular relates to the application of ultraviolet-induced mutation in enhancing the lysing performance of Bdellovibrio. Background technique [0002] For microorganisms in nature, it is difficult to obtain excellent strains if they are simply isolated and purified, and the strains are selected, and generally it is difficult to meet the needs of production. Microbial breeding refers to the use of physical, chemical and other factors to induce variation in the genetic characteristics of microorganisms under artificial conditions, and to select excellent strains with special traits that meet the requirements for cultivation, so that microorganisms can be effectively used in industrial production. [0003] The characteristics of Bdellovibrio that can infect and lyse host bacteria make it suitable as a biological decontamination factor for inhibiting or eliminating pathog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00C12R1/63
Inventor 蔡俊鹏韩晓宁
Owner SOUTH CHINA UNIV OF TECH
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