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Calf chondroprotein, preparation and use thereof

A cartilage protein, calf technology, applied in the preparation method, application, food preparation and other directions of peptides, can solve problems such as restricting development

Inactive Publication Date: 2009-06-24
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported abroad that shark chondroitin extracted from shark cartilage has similar effects, but as a wild animal, the source of sharks limits its development

Method used

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  • Calf chondroprotein, preparation and use thereof
  • Calf chondroprotein, preparation and use thereof
  • Calf chondroprotein, preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of calf cartilage protein

[0028] 10kg of newborn calf ears are peeled, washed, mashed and soaked in 1mol / L 10L acetate buffer overnight, usually for 12-16 hours. Centrifuge to remove the precipitate, take the supernatant and add 2 times the volume of saturated ammonium sulfate for fractional precipitation (the final saturation of ammonium sulfate is 50%). At 4°C for more than 3 hours, make it fully precipitate and centrifuge (3000rpm) for 20 minutes, discard the supernatant, dissolve the precipitate with 1L of normal saline, and then add 500ml of saturated ammonium sulfate dropwise. Put it at 4°C for more than 3h, [at this time, (NH4) 2 The saturation of SO4 is 33%] repeat above-mentioned step process 2 times. The precipitate obtained after the final centrifugation is protein, which is dissolved in 0.02% mol / L pH7.4 PBS to 500 ml and loaded into a dialysis bag. Fully dialyze the PBS and change the solution 3 times until the dialyzed fl...

Embodiment 2

[0029] Embodiment 2: the separation of calf cartilage protein

Embodiment 3

[0031] Embodiment 3: Calf cartilage protein electrophoresis qualitative analysis

[0032]Method reference: Sambrook et al., Molecular Cloning Test Guide, Second Edition, 1995, Science Press, China, Beijing. Weigh 10ml each of calf cartilage protein A, calf cartilage protein B, calf cartilage protein C, calf cartilage protein D, and calf cartilage protein E, and fully dissolve them in a test tube containing 1.0 ml of double distilled water. Prepare 0.02ml of sample solution. The sample and the standard protein were subjected to 12% SDS-polyacrylamide gel electrophoresis, and the electrophoresis condition was 100v for about 1 hour. After electrophoresis, the polyacrylamide gel was stained and decolorized with Coomassie Brilliant Blue R-250 dye. Comparing the obtained results with the standard proteins, the molecular weights of calf cartilage protein A, calf cartilage protein B, calf cartilage protein C, calf cartilage protein D, and calf cartilage protein E are 66Kd, 32Kd, 32K...

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Abstract

The invention belongs to chondroprotein of a neonatal calf, a preparation method and the application. A set of functional protein with activity is obtained by extracting, separating and purifying the chondroprotein of the neonatal calf. The protein has the functions of anti-aging, antitumor and prevention and cure of joint diseases and hyperplasia of lobule of mammary glands; the protein is a health product or the protein can be used for preparing drugs, including oral preparation, such as tablets, spansules, osmotic pump controlled release tablets or dispersing tablets, or including medicinal extracts, pellicles, liposomes, micro-capsules, udder massage thimerosal, and the like.

Description

technical field [0001] The invention belongs to the technical field of multifunctional food, and relates to the research of a multifunctional calf cartilage protein, a preparation method and its application. Background technique [0002] Newborn male cows (fetal cows) are often sacrificed because they are unable to produce milk. At the beginning of this century, the Shanghai Cancer Institute formed a research team consisting of a group of middle and senior scientific and technical personnel to participate in the research on the application and development of calf cartilage-like substances. Extract and purify from calf cartilage tissue to obtain an active protein that not only inhibits neovascularization, but also has anti-oxidation function. After years of research in the central laboratory, the test results have confirmed that there are a series of biologically active proteins in calf cartilage tissue that can inhibit the growth of blood vessels in chicken embryos and resi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/30C07K1/16A23L1/29A61K38/17A61K9/20A61K9/22A61K9/48A61K9/127A61K9/50A61K9/08A61K9/06A61P35/00A61P39/06A61P19/02A61P15/00
Inventor 段友蓉陈复华
Owner SHANGHAI INST OF ONCOLOGY
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