Piggery fermentation bed composite microbial preparation and preparation thereof
A compound microbial inoculum and the technology of compound inoculum, which are applied in the field of compound microbial inoculum, can solve the problems of lack of scientific and effective regulation means, influence effect, lack of theoretical guidance for production process and so on.
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Embodiment 1
[0037] 1. Selection of fermentation strains and preparation of medium
[0038] Bacteria A—Bacillus subtilis (CICC 10088), the culture medium is 1000 milliliters of beef extract, 10 grams of peptone, 5 grams of sodium chloride, and pH 7.2 to 7.4.
[0039] B bacteria——Lactobacillus plantarum (CICC 6026), the medium is 5 ° Bé wort, adding yeast extract 0.5, aseptic calcium carbonate 0.6.
[0040] Bacteria C——Candida utilis (CICC 31170), the medium is 5°Bé wort.
[0041] Bacteria D - Geotrichum candidum (CICC 1315), the medium is 5 ° Bé wort.
[0042] E bacteria - Aspergillus oryzae (CICC 2011), the medium is 5 ° Bé rice koji juice.
[0043] 2. Preparation process of compound bacterial agent
[0044] 2.1 Activation of freeze-dried bacteria powder
[0045] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in ampoules of 5 strains into 0.9% In normal saline, oscillate to make it ...
Embodiment 2
[0058] 1. Selection of fermentation strains and preparation of medium
[0059] Bacteria A—Bacillus subtilis (CICC 10088), the culture medium is 1000 milliliters of beef extract, 10 grams of peptone, 5 grams of sodium chloride, and pH 7.2 to 7.4.
[0060] B bacteria——Lactobacillus plantarum (CICC 6026), the medium is 5 ° Bé wort, adding yeast extract 0.5, aseptic calcium carbonate 0.6.
[0061] Bacteria C——Candida utilis (CICC 31170), the medium is 5°Bé wort.
[0062] Bacteria D - Geotrichum candidum (CICC 1315), the medium is 5 ° Bé wort.
[0063] E bacteria - Aspergillus oryzae (CICC 2011), the medium is 5 ° Bé rice koji juice.
[0064] 2. Preparation process of compound bacterial agent
[0065] 2.1 Activation of freeze-dried bacteria powder
[0066] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in ampoules of 5 strains into 0.9% In normal saline, oscillate to make it ...
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