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Piggery fermentation bed composite microbial preparation and preparation thereof

A compound microbial inoculum and the technology of compound inoculum, which are applied in the field of compound microbial inoculum, can solve the problems of lack of scientific and effective regulation means, influence effect, lack of theoretical guidance for production process and so on.

Inactive Publication Date: 2009-06-24
HEILONGJIANG ZENONG COLD AREA BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the bacterial agents used in domestic fermentation bed pig farming are collected by hand and added value through natural cultivation. There is a lack of scientific and effective control methods, and the quality of the products cannot be guaranteed.
[0004] At present, some units in our country have successively launched different combinations of compound microbial preparations for pig farming in fermented beds, but the common problem is that in the process of screening and cultivating compound microorganisms, there is a lack of basic theoretical guidance and mature production technology. The interaction and mechanism between its individual flora are relatively unfamiliar, resulting in the total number of viable bacteria in the product cannot be maintained at a certain level, and the product quality is unstable, which affects its effect during use

Method used

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  • Piggery fermentation bed composite microbial preparation and preparation thereof

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Comparison scheme
Effect test

Embodiment 1

[0037] 1. Selection of fermentation strains and preparation of medium

[0038] Bacteria A—Bacillus subtilis (CICC 10088), the culture medium is 1000 milliliters of beef extract, 10 grams of peptone, 5 grams of sodium chloride, and pH 7.2 to 7.4.

[0039] B bacteria——Lactobacillus plantarum (CICC 6026), the medium is 5 ° Bé wort, adding yeast extract 0.5, aseptic calcium carbonate 0.6.

[0040] Bacteria C——Candida utilis (CICC 31170), the medium is 5°Bé wort.

[0041] Bacteria D - Geotrichum candidum (CICC 1315), the medium is 5 ° Bé wort.

[0042] E bacteria - Aspergillus oryzae (CICC 2011), the medium is 5 ° Bé rice koji juice.

[0043] 2. Preparation process of compound bacterial agent

[0044] 2.1 Activation of freeze-dried bacteria powder

[0045] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in ampoules of 5 strains into 0.9% In normal saline, oscillate to make it ...

Embodiment 2

[0058] 1. Selection of fermentation strains and preparation of medium

[0059] Bacteria A—Bacillus subtilis (CICC 10088), the culture medium is 1000 milliliters of beef extract, 10 grams of peptone, 5 grams of sodium chloride, and pH 7.2 to 7.4.

[0060] B bacteria——Lactobacillus plantarum (CICC 6026), the medium is 5 ° Bé wort, adding yeast extract 0.5, aseptic calcium carbonate 0.6.

[0061] Bacteria C——Candida utilis (CICC 31170), the medium is 5°Bé wort.

[0062] Bacteria D - Geotrichum candidum (CICC 1315), the medium is 5 ° Bé wort.

[0063] E bacteria - Aspergillus oryzae (CICC 2011), the medium is 5 ° Bé rice koji juice.

[0064] 2. Preparation process of compound bacterial agent

[0065] 2.1 Activation of freeze-dried bacteria powder

[0066] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in ampoules of 5 strains into 0.9% In normal saline, oscillate to make it ...

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Abstract

The invention provides a pigsty fermenting bed compound microbial fertilizer and a preparation method thereof. The method comprises: first, five strains of bulk starters which are bacillus subtilis, lactobacillus plantarum, candida utili, geotrichum candidum and aspergillus oryzae, and the viable count of the bulk starters is more than or equal to 10<9>cfu / ml; protective agent with proper amount is added into the five strains of bulk starters for freeze drying; according to the parts by weight of the freeze-dried bacteria powder, 2-4 parts of bacillus subtilis powder, 3-5 parts of lactobacillus plantarum powder, 3-5 parts of candida utili powder, 2-4 parts of geotrichum candidum powder and 3-5 parts of aspergillus oryzae powder are fully mixed together and then packaged by vacuum, so that the compound microbial fertilizer is obtained. When 5-20g / m<2> of the compound microbial fertilizer is used in the pigsty fermenting bed, the odor of the pigsty can be obviously reduced, the growth chance of flies can be decreased and the sick frequency of the pigs can be reduced, so that the pigs can grow up healthily and green pork can be provided for the human beings while the environment is protected.

Description

(1) Technical field [0001] The invention relates to a composite microbial bacterial agent, in particular to a composite microbial bacterial agent used in a piggery fermentation bed. The invention also relates to a preparation method of the composite microbial bacterial agent. (2) Background technology [0002] The annual growth rate of breeding scale and output value in our country exceeds 10%, but at the same time, the environmental pollution brought by the breeding industry is becoming more and more serious. At present, rural areas and agriculture have economic organization and management methods based on family dispersal. Although large-scale breeding is the development direction of the aquaculture industry, for a long time, family or small-scale decentralized farming methods will not be fundamental. At the same time, due to the small-scale or scattered farming methods, the management of pollution prevention and control is extensive and large, and the problem of fecal po...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/16A01K1/015C12R1/125C12R1/25C12R1/72C12R1/69C12R1/645
Inventor 赵云财张超李娜李泽奇邹东恢
Owner HEILONGJIANG ZENONG COLD AREA BIOLOGICAL ENG
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