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Application of cumarin coumpound Murraya jasminorage ketone in preparing anti-tumor medicament

An anti-tumor drug and a technology of coumarins, which are applied in the field of medicine and achieve the effects of good development and utilization prospects, high safety and low price

Inactive Publication Date: 2009-07-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Jiulixiang ketone has good antibacterial and anti-inflammatory effects (1. Zou Lianxin, Zheng Hanchen, Yang Ziren. Research progress of Jiulixiang plants. Journal of Pharmaceutical Practice. 1997; 15(4): 214-219.2. Yan Jie, Pei Jianmei. HPLC method Determination of Jiulixiangone Content in Sanjiuweitai Granules. Chinese Herbal Medicine. 2005; 36(3): 375-376.3. Sui Dayuan, Li Shuhui. Survey of Jiulixiang Chemical and Anti-fertility Research. Chinese Herbal Medicine. 1994; 25(8) : 435-437, 448.), but so far there is no report about its anti-tumor activity

Method used

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  • Application of cumarin coumpound Murraya jasminorage ketone in preparing anti-tumor medicament
  • Application of cumarin coumpound Murraya jasminorage ketone in preparing anti-tumor medicament
  • Application of cumarin coumpound Murraya jasminorage ketone in preparing anti-tumor medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Inhibition of tumor cell growth by Jiulixiangone (MTT method)

[0012] Cell line:

[0013] Using five tumor cell lines that are routinely used: human leukemia cell line K562, human liver cancer cell lines Hep3B and HepG2, human colon cancer cell line SW480 and human cervical cancer cell line HeLa, by Shanghai Dongfang Hepatobiliary Surgery Hospital Tumor Immunity and Gene Therapy Research Provided by the center (the same below).

[0014] Jiulixiangone:

[0015] Purchased from Chengdu Pusi Biotechnology Co., Ltd., the purity is greater than 98% (HPLC method, the same below). The DMEM culture solution containing 10% calf serum by volume or the RPMI1640 culture solution containing 10% calf serum by volume were used to prepare different concentrations of Jiulixiangone solutions, the concentrations were 5, 10, 20, 40 , 80mg / mL.

[0016] 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT):

[0017] Purchased from Sigma-Aldrich Company, prepared 1mg...

Embodiment 2

[0020] Example 2 Experiment of the inhibitory effect of jirixiangone on the growth of animal subcutaneous xenograft tumors

[0021] animal:

[0022] Kunming mice, half male and half male, weighing 18g±2g, were provided by the Animal Center of Shanghai Medical University. The animals were fed with pellet feed, and had free access to food and water (the same below).

[0023] Cell line:

[0024] H22 mouse liver cancer cell line, HepS mouse liver cancer cell line: provided by the Shanghai Cell Institute of the Chinese Academy of Sciences.

[0025] Cyclophosphamide powder injection (CTX): product of Shanghai Hualian Pharmaceutical Co., Ltd.

[0026] Jiulixiangone: Same as above.

[0027] Trypan blue:

[0028] Trypan blue staining cell viability detection kit: purchased from Shanghai Biyuntian Biotechnology Co., Ltd.

[0029] experimental method:

[0030] Eight female mice were randomly divided into two groups, and the H22 mouse liver cancer cell line and the HepS mouse liver...

Embodiment 3

[0038] Example 3 Acute Toxicity Test of Jiulixiangone

[0039] According to the routine, the maximum solubility of the drug is used to give the mice the maximum dosage capacity. According to the body weight, a single gavage of jiurixiangone 2.5g / kg, or a single intraperitoneal injection of jiurixiangone 1.5g / kg, observe the animals after administration. Toxic reaction and death within 14 days. The result is as follows:

[0040] 1) There was no abnormality within 4 hours after a single intragastric administration of the mice, and within 14 days after the administration, the mice did not die. On the 15th day, all the mice were sacrificed, dissected, and all organs were inspected with the naked eye. Obvious lesions.

[0041] 2) 20 minutes after the intraperitoneal injection of the mice, 3 mice appeared listless, curled up, and decreased in activity. One hour after the administration, all the mice returned to normal. Within 14 days after the administration, the mice did not die....

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PUM

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Abstract

The invention relates to the field of medical technology, more particularly to a new use of coumarin compound murrayone in the preparation of antineoplastic drugs. As expressed by in vivo and in vitro experiments, the murrayone has inhibition effect on both the growth of tumor cells and the growth of subcutaneous transplantable tumor of animals, and the experiment for the acute toxicity of animals shows that the acute toxicity of the murrayone is quite low, therefore, the invention can be used for preparing the antineoplastic drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the new application of coumarin compound jiurigenone in preparing antitumor drugs. Background technique [0002] Murrayone (murrayone) is a coumarin compound, which can be extracted from Murraya exotica L. The method of semi-synthesizing the precursors of compound-like compounds (Pharmacopoeia Commission of the Ministry of Health of the People's Republic of China. Pharmacopoeia of the People's Republic of China Part I. 2005: 9.). The chemical structural formula of Jiulixiangone is as follows: [0003] [0004] Jiulixiang ketone has good antibacterial and anti-inflammatory effects (1. Zou Lianxin, Zheng Hanchen, Yang Ziren. Research progress of Jiulixiang plants. Journal of Pharmaceutical Practice. 1997; 15(4): 214-219.2. Yan Jie, Pei Jianmei. HPLC method Determination of Jiulixiangone Content in Sanjiuweitai Granules. Chinese Herbal Medicine. 2005; 36(3): 375-376.3. Sui Day...

Claims

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Application Information

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IPC IPC(8): A61K31/37A61P35/00
Inventor 张黎刘厚佳王晓黎高平挥卫立辛芮耀诚吴孟超
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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