Method for degrading ligno-cellulose and special cellulose decomposing bacteria therefor

A technology of cellulolytic bacteria and lignocellulose, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of difficult to achieve pure culture of microorganisms, obtain sample microbial population information, etc., to achieve Promote the development of animal husbandry, significant economic value and social significance, and the effect of maintaining soil fertility

Inactive Publication Date: 2009-07-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, most studies on microbial diversity and microbial population composition used traditional isolation and culture methods, but some types of microorganisms are difficult to achieve pure culture, so it is difficult to use traditional microbial isolation and culture techniques to obtain complete microbial population information in samples

Method used

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  • Method for degrading ligno-cellulose and special cellulose decomposing bacteria therefor
  • Method for degrading ligno-cellulose and special cellulose decomposing bacteria therefor
  • Method for degrading ligno-cellulose and special cellulose decomposing bacteria therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the acquisition of cellulolytic strain L-A

[0028] Each liter of medium contains the following components: yeast powder, 5g; casein peptone, 1.5g; K 2 HPO 4 , 1.5g; MgSO 4 ·7H 2 O, 0.5g; CaCl 2 ·H 2 O, 1 g; NaHCO 3 , 0.9g; NaCl, 0.6g; NH 4 Cl, 0.6g; FeCl 3 , 0.006g; pH7.2.

[0029] The preparation method of the medium is: FeCl 3 Prepared as a 1g / L storage solution. Weigh the remaining corresponding medicine in proportion and pour it into a beaker filled with tap water in advance. After the medicine is fully dissolved, dilute to 990ml with tap water, and add 6ml of FeCl 3 Store the solution, adjust the pH to 7.2, and finally adjust the volume to 1000ml.

[0030] Distribute each 10ml of culture medium into 18×180mm common test tubes (with 12×100mm filter paper strips placed in advance).

[0031] 1. Obtaining of cellulolytic strain L-A

[0032] 0.5 g of anaerobic activated sludge sample collected from Guangxi cassava alcohol waste liquid UASB-TL...

Embodiment 2

[0078] Embodiment 2, the culture temperature optimization of cellulolytic strain L-A

[0079] Each liter of medium contains the following components: yeast powder, 5g; casein peptone, 1.5g; K 2 HPO 4 , 1.5g; MgSO 4 ·7H 2 O, 0.5g; CaCl 2 ·H 2 O, 1 g; NaHCO 3 , 0.9g; NaCl, 0.6g; NH 4 Cl, 0.6g; FeCl 3 , 0.006g; pH7.2.

[0080] The preparation method of the medium is: FeCl 3 Prepared as a 1g / L storage solution. Weigh the remaining corresponding medicine in proportion and pour it into a beaker filled with tap water in advance. After the medicine is fully dissolved, dilute to 990ml with tap water, and add 6ml of FeCl 3 Store the solution, adjust the pH to 7.2, and finally adjust the volume to 1000ml.

[0081] Distribute each 10ml of culture medium into 18×180mm common test tubes (with 12×100mm filter paper strips placed in advance).

[0082] The cellulolytic strain L-A obtained in step 1 of Example 1 was inoculated into test tubes containing medium, the inoculum amount w...

Embodiment 3

[0084] Embodiment 3, cellulolytic strain L-A degrades lignocellulose

[0085]Each liter of medium contains the following components: yeast powder, 1g; casein peptone, 1g; K 2 HPO 4 , 0.1g; MgSO 4 ·7H 2 O, 0.1g; CaCl 2 ·H 2 O, 0.5g; NaHCO 3 , 0.2g; NaCl, 0.1g; NH 4 Cl, 0.1g; FeCl 3 , 0.001g; pH6.8.

[0086] The preparation method of the medium is: FeCl 3 Prepared as a 1g / L storage solution. Weigh the remaining corresponding medicine in proportion and pour it into a beaker filled with tap water in advance. After the medicine is fully dissolved, use tap water to make the volume to 990ml, and add 1ml of FeCl 3 Store the solution, adjust the pH to 6.8, and finally adjust the volume to 1000ml.

[0087] Distribute each 10ml of culture medium into 18×180mm common test tubes.

[0088] Take 30 test tubes filled with culture medium and divide them into three groups, 10 tubes in each group. Dried straw was added to the first set of test tubes (0.1 g of dried straw was added t...

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PUM

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Abstract

The invention discloses a lignocellulose decomposing method and a specific cellulose decomposing fungus strain thereof. The invention provides a cellulose decomposing fungus strain L-A with CCTCC NO. of M208163, and further provides a culture medium that is used for cultivating the cellulose decomposing fungus strain L-A with CCTCC NO. of M208163, as well as applications of the cellulose decomposing fungus strain L-A with CCTCC NO. of M208163 in lignocellulose decomposition. The lignocellulose decomposing method is favorable for solving the current situation that lignocellulose resource cannot reach complete utilization. The lignocellulose that is preliminarily decomposed by the cellulose decomposing fungus strain L-A can be used as fertilizer for improving and reserving soil fertility, as poultry feedstuff for promoting the development of animal husbandry, and as raw materials of marsh gas fermentation and ethanol production for obtaining clean energies. The lignocellulose decomposing method and the specific cellulose decomposing fungus strain transform cheap regenerative plant cellulose resources into environment-friendly products, thus changing waste into valuables and having great economic value and social significance.

Description

technical field [0001] The invention relates to a method for degrading lignocellulose and a special cellulolytic strain thereof. Background technique [0002] Lignocellulose is mainly composed of three components: cellulose, hemicellulose and lignin, which combine with each other to form complex supramolecular complexes. Lignocellulose is the most abundant biomass resource in my country, including a large amount of crop straw, industrial and forestry waste, and municipal solid waste. At present, most lignocellulose resources are not fully utilized. Especially crop straw, which has the characteristics of large yield, wide distribution and many varieties, has great application potential. After lignocellulose is decomposed into small molecular organic matter, it can be used as fertilizer to improve and maintain soil fertility, as livestock feed to promote the development of animal husbandry, and as raw material for biogas fermentation and ethanol production to obtain clean en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/22C12R1/08C12R1/01
Inventor 杨苏声刘文彦程序梁近光朱万斌王磊
Owner CHINA AGRI UNIV
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