Method for degrading lignocellulose and special cellulose degradation bacteria thereof

A technology of cellulolytic bacteria and lignocellulose, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of obtaining sample microbial population information and the difficulty of pure culture of microorganisms, so as to promote animal husbandry Development, significant economic value and social significance, effect of maintaining soil fertility

Inactive Publication Date: 2011-06-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, most studies on microbial diversity and microbial population composition used traditional isolation and culture methods, but some types of microorganisms are difficult to achieve pure culture, so it is difficult to use traditional microbial isolation and culture techniques to obtain complete microbial population information in samples

Method used

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  • Method for degrading lignocellulose and special cellulose degradation bacteria thereof
  • Method for degrading lignocellulose and special cellulose degradation bacteria thereof
  • Method for degrading lignocellulose and special cellulose degradation bacteria thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the acquisition of cellulolytic strain L-C

[0025] Each liter of medium contains the following components: yeast extract, 2g; tryptone, 2g; NaCl, 18g; KCl, 1g; MgSO 4 ·7H 2 O, 6g; MgCl 2 ·6H 2 O, 4g; CaCl 2 ·H 2 O, 1.5g; NaHCO 3 , 0.7g; pH7.2.

[0026] Weigh the remaining corresponding medicine in proportion and pour it into a beaker filled with some tap water in advance. After the medicine is fully dissolved, use tap water to set the volume to 990ml, adjust the pH to 7.2, and finally set the volume to 1000ml.

[0027] Distribute each 10ml of culture medium into 18×180mm common test tubes (with 12×100mm filter paper strips placed in advance).

[0028] 1. Acquisition of cellulolytic strain L-C

[0029] Inoculate 0.2-0.6 anaerobic activated sludge samples collected from Guangxi cassava alcohol waste liquid UASB-TLP treatment system into test tubes containing medium. The test tubes were shaken and cultured at a temperature of 40-50° C. at a speed of...

Embodiment 2

[0074] Embodiment 2, the culture temperature optimization of cellulolytic strain L-C

[0075] Each liter of medium contains the following components: yeast extract, 2g; tryptone, 2g; NaCl, 18g; KCl, 1g; MgSO 4 ·7H 2 O, 6g; MgCl 2 ·6H 2 O, 4g; CaCl 2 ·H 2 O, 1.5g; NaHCO 3 , 0.7g; pH7.2.

[0076] The preparation method of the culture medium is as follows: Weigh the remaining corresponding medicine in proportion and pour it into a beaker filled with tap water in advance, after the medicine is fully dissolved, use tap water to adjust the volume to 990ml, adjust the pH to 7.2, and finally adjust the volume to 1000ml.

[0077] Distribute each 10ml of culture medium into 18×180mm common test tubes (with 12×100mm filter paper strips placed in advance).

[0078] The cellulolytic strain L-C obtained in step 1 of Example 1 was inoculated into test tubes containing culture medium, the inoculum amount was 5% (volume percentage), and 40 test tubes were inoculated. The 40 samples wer...

Embodiment 3

[0080] Embodiment 3, cellulolytic strain L-C degrades lignocellulose

[0081] Each liter of medium contains the following components: yeast extract, 1g; tryptone, 1g; NaCl, 10g; KCl, 0.3g; MgSO 4 ·7H 2 O, 3g; MgCl 2 ·6H 2 O, 2g; CaCl 2 ·H 2 O, 0.5g; NaHCO 3 , 0.2g.

[0082] The preparation method of the culture medium is as follows: Weigh the remaining corresponding medicine in proportion and pour it into a beaker filled with tap water in advance. After the medicine is fully dissolved, use tap water to adjust the volume to 990ml, adjust the pH to 6.8, and finally adjust the volume to 1000ml.

[0083] Distribute each 10ml of culture medium into 18×180mm common test tubes.

[0084] Take 60 test tubes filled with culture medium and divide them into six groups, 10 in each group. Add dried straw to the first and second test tubes (add 0.1 g dried straw to each test tube), add dried bagasse to the third and fourth test tubes (add 0.3 g to each test tube bagasse), place 0.3g...

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Abstract

The invention discloses a method for degrading lignocellulose and special cellulose degradation bacteria thereof, and provides cellulose degradation bacteria L-C CCTCC NO: M208165, a culture medium used for culturing the cellulose degradation bacteria L-C CCTCC NO: M208156 and an application of the cellulose degradation bacteria L-C CCTCC NO: M208165 in the process of degrading the lignocellulose. The invention is conductive to solving the problem that the lignocellulose resource can not be absorbed fully in the prior art. The lignocellulose degraded preliminarily by the cellulose degradationbacteria in the invention can be used as fertilizer to improve and maintain soil fertility, or used as animal feed to enhance the development of animal husbandry, or as the raw materials for biogas fermentation and ethanol production to obtain clean energy. The invention can transfer the low-cost renewable plant cellulose resources into environmental-friendly products, makes waste profitable and has significant economic values and social significance.

Description

technical field [0001] The invention relates to a method for degrading lignocellulose and a special cellulolytic strain thereof. Background technique [0002] Lignocellulose is mainly composed of three components: cellulose, hemicellulose and lignin, which combine with each other to form complex supramolecular complexes. Lignocellulose is the most abundant biomass resource in my country, including a large amount of crop straw, industrial and forestry waste, and municipal solid waste. At present, most lignocellulose resources are not fully utilized. Especially crop straw, which has the characteristics of large yield, wide distribution and many varieties, has great application potential. After lignocellulose is decomposed into small molecular organic matter, it can be used as fertilizer to improve and maintain soil fertility, as livestock feed to promote the development of animal husbandry, and as raw material for biogas fermentation and ethanol production to obtain clean en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/10C12P5/02A23K1/12C05F11/00C12R1/01C12R1/07A23K10/32
CPCY02E50/16Y02E50/10
Inventor 杨苏声刘文彦程序梁近光朱万斌
Owner CHINA AGRI UNIV
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