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Nucleic acid molecule SI-CYPJ-12 and use thereof in anti-cancer medicine preparation

An anti-tumor drug and molecular technology, applied in the field of biomedicine, can solve the problems of limited application, lack of tumor cell specificity, insensitivity to toxic and side effects chemotherapy and radiotherapy, etc., and achieve the effect of inhibiting tumor cell proliferation.

Inactive Publication Date: 2009-08-12
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemotherapy and radiotherapy are still the first choice for the treatment of cancer. Although the two have achieved considerable curative effect on the treatment of tumors, they have relatively large toxic and side effects due to the lack of specificity to tumor cells, and some tumor cells have a certain effect on chemotherapy. and insensitivity to radiotherapy, thus limiting their clinical application to a large extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 SI-CYPJ-12 inhibits tumor cell proliferation

[0023] LV-CYPJ-RNAi is a viral vector carrying a small hairpin RNA (sh-RNA) targeting CYPJ, while LV-non-silencing is a viral vector carrying a control fragment. Lentivirus was purchased from Shanghai Jikai Gene Technology Co., Ltd.

[0024] Virus-infected liver cancer cell line SK-Hep1: (A) The day before the experiment, 3-5×10 3 The target cells were placed in a 96-well culture plate, and the volume of the medium added was 100 μl. (B) During the experiment, the virus stored at -70°C was thawed on ice, and the virus was diluted as needed. (C) Take out the cells from the incubator and discard the original culture medium, wash the cells with 50 μl of serum-free medium, then add 50 μl of serum-free medium, and then add 50 μl of virus dilution. The total volume of the culture medium is 100 μl, mix well and place at 37°C for incubation. After 48 hours, it was replaced with normal medium containing serum. (D) GFP ...

Embodiment 2

[0025] Example 2 Inhibition of tumor growth in nude mice

[0026] 2.1 Tumor formation in nude mice (Shanghai Slack Experimental Animal Co., Ltd.): Mass culture and detection of SK-Hep1 cells, digest with trypsin, harvest the cultured cells by centrifugation, wash with PBS, count, resuspend in PBS, and use the cells once Subcutaneously inject nude mice with sex injector, inject ≥5 mice in each group, (female, 30-40 days), the cell injection volume of each nude mouse is 200μl, and the cell number is about 2×10 6 , a week later the tumor grew.

[0027] 2.2 Virus injection: when the tumor volume grows to 10-30mm 3 volume, 20 μl containing 3 x 10 7 TU titer virus in PBS was injected directly into the tumor with a syringe. The experimental group was injected with LV-CYPJ-RNAi virus, and the control group was injected with LV-non-silencing virus. Two days later, the virus was injected again with the same method and dosage.

[0028] 2.3 Tumor monitoring and tumor mass removal: Th...

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PUM

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Abstract

The invention belongs to the biological medicine field, relating to nucleotide molecules and the application thereof in the preparation of antitumor medicines. The invention provides the nucleotide molecules for preparing the antitumor medicines. The nucleotide molecules have the activity for inhibiting the tumor growth and the sequence thereof comprises 5'-GUGUUGCCUCCUCUUCCAG-3'or 5'-GTGTTGCCTCCTCTTCCAG-3' which is known as SI-CYPJ-12 in the invention. The nucleotide molecules SI-CYPJ-12 can obviously inhibit the tumor cell proliferation; when the nucleotide molecules are applied to a naked mouse, the tumor body growth of the naked mouse is obviously inhibited compared with the control naked mouse in the same condition of culture, and the phenomenon becomes obvious day by day as time goes on. The invention provides a new approach and means for the tumor treatment and mitigation.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a nucleotide molecule and its application in the preparation of antitumor drugs. Background technique [0002] Tumor disease has now risen to be the No. 2 "killer" in the world, and its death toll is second only to cardiovascular disease. Over the past few years, the foreign medical community has gained a new understanding of the pathogenesis of tumor diseases on the basis of cells. Based on the further understanding of the pathogenesis of tumors, people use various approaches to research and develop drugs that can specifically and effectively kill tumor cells without toxicity to normal cells. At present, chemotherapy and radiotherapy are still the first choice for the treatment of cancer. Although the two have achieved considerable curative effect on the treatment of tumors, they have relatively large toxic and side effects due to the lack of specificity to tumor cells, and some tumor c...

Claims

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Application Information

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IPC IPC(8): C07H21/02A61K48/00A61K31/7088A61P35/00
Inventor 余龙陈帅张明君
Owner FUDAN UNIV
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