Application of carex rigescens for preparing anti-HIV drug
A technology of Carex sylvestris and medicine, applied in the field of application of Carex syringae in preparation of anti-HIV drugs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] Embodiment 1: prepare a kind of anti-HIV medicine, the steps are as follows:
[0026] (1) Extraction with organic solvent: crush 100Kg of dry Carex alba, put it into 200L of 30% acetone aqueous solution, soak at room temperature for 12 hours, repeat the extraction twice, combine the extracts, and distill off the organic solvent under reduced pressure to obtain an aqueous suspension.
[0027] (2) Adjust the pH value of the above water suspension to 4 with acetic acid, let stand at room temperature for 30 minutes, and then centrifuge to obtain a precipitate.
[0028] (3) Normal-phase chromatographic column separation: the above-mentioned precipitate is chromatographed on a silica gel column, eluted with a chloroform-methanol gradient, and the eluent whose volume ratio of chloroform and methanol is 50:50 to 10:90 is collected, and distilled under reduced pressure , Concentrate, get the normal phase chromatographic column separation product.
[0029] (4) Reversed-phase chr...
Embodiment 2
[0032] Embodiment 2: prepare a kind of anti-HIV medicine, the steps are as follows:
[0033] (1) Organic solvent extraction: crush 200 g of dried Carex alba, put it into 400 mL of 70% ethanol aqueous solution, ultrasonically extract for 3 hours, repeat the extraction twice, combine the extracts, and distill off the organic solvent under reduced pressure to obtain an aqueous suspension.
[0034] (2) Adjust the pH value of the above water suspension to 4 with acetic acid, let stand at room temperature for 30 minutes, and then centrifuge to obtain a precipitate.
[0035] (3) Normal-phase chromatographic column separation: the above-mentioned precipitate is chromatographed on a silica gel column, eluted with a chloroform-methanol gradient, and the eluent whose volume ratio of chloroform and methanol is 50:50 to 10:90 is collected, and distilled under reduced pressure , Concentrate, get the normal phase chromatographic column separation product.
[0036] (4) Reversed-phase chromat...
Embodiment 3
[0038] Embodiment 3: prepare a kind of anti-HIV medicine, the steps are as follows:
[0039] (1) Extraction with organic solvent: crush 200 g of dried Carex alba, put it into 400 mL of 60% acetone aqueous solution, soak at room temperature for 14 hours, repeat the extraction twice, combine the extracts, and distill off the organic solvent under reduced pressure to obtain an aqueous suspension.
[0040] (2) Adjust the pH value of the above water suspension to 4 with acetic acid, let stand at room temperature for 30 minutes, and then centrifuge to obtain a precipitate.
[0041] (3) Normal-phase chromatographic column separation: the above-mentioned precipitate is chromatographed on a silica gel column, eluted with a chloroform-methanol gradient, and the eluent whose volume ratio of chloroform and methanol is 50:50 to 10:90 is collected, and distilled under reduced pressure , Concentrate, get the normal phase chromatographic column separation product.
[0042] (4) Reversed-phase...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com