Method for preparing pterocarpus indicus water extract used for endothelium-dependent vasodilation agent and applications of method

Abstract

The invention relates to a method for preparing pterocarpus santalinus water extract used for endothelium-dependent vasodilator and applications of the extract on the medicine or health products of endothelium-independent vasodilatior. The preparation method comprises the steps of extracting the pterocarpus santalinus powders thrice in a back-flow way by 70% of water of 6 times, 5 times and 3 times respectively for 2.5h, 2h and 1.5h, decompressing, concentrating, cooling and drying the extract, and finally obtaining the pterocarpus santalinus extract. Suitable auxiliary material is added into the pterocarpus santalinus extract so as to prepare the medicines or health products such as powder injection, drops, troche, capsule, oral liquid, grain agents and the like.

Description

technical field

[0001] The invention relates to a water extract of sandalwood and pterostilbene used as a direct vasodilator, and the application of the extract in medicines or health products for treating cardiovascular diseases such as hypertension. Background technique

[0002] In addition to serving as a physiological barrier between blood and vascular smooth muscle, the vascular endothelium also has an active metabolic endocrine function to maintain the balance of the vascular environment. Endothelium-dependent Vasodilation (EDD) is one of the important functions of vascular endothelium, and its dysfunction plays an important role in the early formation, development and complications of atherosclerosis (AS), especially cardiovascular diseases. leading role.

[0003] There are two ways of vasodilation: EDD: ① Refers to endothelial cells releasing EDRF under the action of chemical substances such as acetylcholine, bradykinin, etc. or physiological stimuli (such as reacti...

Examples

Embodiment 1

[0023] Embodiment 1: concentration is the vasoconstriction effect of 0.0025g / ml water extract of Pterocarpus sandalwood dependent inhibition of phenylephrine

[0024] experiment method:

[0025] The mouse thoracic aorta ring has been used in the present experiment. The preparation was oxidized with saline solution, and then mixed with the following composition: ① sodium chloride (119); ② potassium chloride (4.7); ③ potassium dihydrogen phosphate (1.2); ④ magnesium sulfate (1.2); Sodium hydrogen (25); ⑥ calcium chloride (1.6); ⑦ glucose (11), keep the temperature at 4 degrees Celsius. Prepare two arterial rings (each 4 mm long) and remove the endothelium by gently rubbing the inner surface with a smooth wooden stick. Successful endothelium removal can be confirmed by meeting the aortic ring with phenylephrine (1 μM) lack of a relaxing response to acetylcholine. Isovolumetric stretches of the aortic annulus were recorded using a computerized myograph system. After equilibrat...

Embodiment 2

[0029] Embodiment 2: concentration is the vasoconstriction effect of 0.0035g / ml water extract of pterostilbene dependent inhibition of phenylephrine

[0030] experiment method:

[0031] The mouse thoracic aorta ring has been used in the present experiment. The preparation was oxidized with saline solution, and then mixed with the following composition: ① sodium chloride (119); ② potassium chloride (4.7); ③ potassium dihydrogen phosphate (1.2); ④ magnesium sulfate (1.2); Sodium hydrogen (25); ⑥ calcium chloride (1.6); ⑦ glucose (11), keep the temperature at 4 degrees Celsius. Prepare two arterial rings (each 4 mm long) and remove the endothelium by gently rubbing the inner surface with a smooth wooden stick. Successful endothelium removal can be confirmed by meeting the aortic ring with phenylephrine (1 μM) lack of a relaxing response to acetylcholine. Isovolumetric stretches of the aortic annulus were recorded using a computerized myograph system. After equilibration with ...

Embodiment 3

[0035] Embodiment 3: concentration is the vasoconstriction effect of the water extract of 0.005g / ml Pterocarpus sandalwood dependent inhibition of phenylephrine

[0036] experiment method:

[0037] The mouse thoracic aorta ring has been used in the present experiment. The preparation was oxidized with saline solution, and then mixed with the following composition: ① sodium chloride (119); ② potassium chloride (4.7); ③ potassium dihydrogen phosphate (1.2); ④ magnesium sulfate (1.2); Sodium hydrogen (25); ⑥ calcium chloride (1.6); ⑦ glucose (11), keep the temperature at 4 degrees Celsius. Prepare two arterial rings (each 4 mm long) and remove the endothelium by gently rubbing the inner surface with a smooth wooden stick. Successful endothelium removal can be confirmed by meeting the aortic ring with phenylephrine (1 μM) lack of a relaxing response to acetylcholine. Isovolumetric stretches of the aortic annulus were recorded using a computerized myograph system. After equilib...

technical field

[0001] The invention relates to a water extract of sandalwood and pterostilbene used as a direct vasodilator, and the application of the extract in medicines or health products for treating cardiovascular diseases such as hypertension. Background technique

[0002] In addition to serving as a physiological barrier between blood and vascular smooth muscle, the vascular endothelium also has an active metabolic endocrine function to maintain the balance of the vascular environment. Endothelium-dependent Vasodilation (EDD) is one of the important functions of vascular endothelium, and its dysfunction plays an important role in the early formation, development and complications of atherosclerosis (AS), especially cardiovascular diseases. leading role.

[0003] There are two ways of vasodilation: EDD: ① Refers to endothelial cells releasing EDRF under the action of chemical substances such as acetylcholine, bradykinin, etc. or physiological stimuli (such as reacti...

Examples

Embodiment 1

[0023] Embodiment 1: concentration is the vasoconstriction effect of 0.0025g / ml water extract of Pterocarpus sandalwood dependent inhibition of phenylephrine

[0024] experiment method:

[0025] The mouse thoracic aorta ring has been used in the present experiment. The preparation was oxidized with saline solution, and then mixed with the following composition: ① sodium chloride (119); ② potassium chloride (4.7); ③ potassium dihydrogen phosphate (1.2); ④ magnesium sulfate (1.2); Sodium hydrogen (25); ⑥ calcium chloride (1.6); ⑦ glucose (11), keep the temperature at 4 degrees Celsius. Prepare two arterial rings (each 4 mm long) and remove the endothelium by gently rubbing the inner surface with a smooth wooden stick. Successful endothelium removal can be confirmed by meeting the aortic ring with phenylephrine (1 μM) lack of a relaxing response to acetylcholine. Isovolumetric stretches of the aortic annulus were recorded using a computerized myograph system. After equilibrat...

Embodiment 2

[0029] Embodiment 2: concentration is the vasoconstriction effect of 0.0035g / ml water extract of pterostilbene dependent inhibition of phenylephrine

[0030] experiment method:

[0031] The mouse thoracic aorta ring has been used in the present experiment. The preparation was oxidized with saline solution, and then mixed with the following composition: ① sodium chloride (119); ② potassium chloride (4.7); ③ potassium dihydrogen phosphate (1.2); ④ magnesium sulfate (1.2); Sodium hydrogen (25); ⑥ calcium chloride (1.6); ⑦ glucose (11), keep the temperature at 4 degrees Celsius. Prepare two arterial rings (each 4 mm long) and remove the endothelium by gently rubbing the inner surface with a smooth wooden stick. Successful endothelium removal can be confirmed by meeting the aortic ring with phenylephrine (1 μM) lack of a relaxing response to acetylcholine. Isovolumetric stretches of the aortic annulus were recorded using a computerized myograph system. After equilibration with ...

Embodiment 3

[0035] Embodiment 3: concentration is the vasoconstriction effect of the water extract of 0.005g / ml Pterocarpus sandalwood dependent inhibition of phenylephrine

[0036] experiment method:

[0037] The mouse thoracic aorta ring has been used in the present experiment. The preparation was oxidized with saline solution, and then mixed with the following composition: ① sodium chloride (119); ② potassium chloride (4.7); ③ potassium dihydrogen phosphate (1.2); ④ magnesium sulfate (1.2); Sodium hydrogen (25); ⑥ calcium chloride (1.6); ⑦ glucose (11), keep the temperature at 4 degrees Celsius. Prepare two arterial rings (each 4 mm long) and remove the endothelium by gently rubbing the inner surface with a smooth wooden stick. Successful endothelium removal can be confirmed by meeting the aortic ring with phenylephrine (1 μM) lack of a relaxing response to acetylcholine. Isovolumetric stretches of the aortic annulus were recorded using a computerized myograph system. After equilib...