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Preparation method of water-soluble silk glue with different molecular weights

A water-soluble, molecular weight technology, applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of increasing operational complexity and cost, impact and environmental pollution, and achieve environmental protection, low equipment requirements, Simple preparation process

Inactive Publication Date: 2009-09-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sericin prepared by the above method has different molecular weight distributions and properties, but the addition of reagents such as alkali, organic solvents and enzymes increases the complexity and cost of the operation, and also causes the impact on the sericin and environmental pollution

Method used

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  • Preparation method of water-soluble silk glue with different molecular weights

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take several dry cocoon layers, remove the stolen goods and cut into pieces, weigh a certain amount of broken cocoon pieces, put them into a beaker containing 30 times the volume (V / W) of boiling distilled water, and heat and boil for 30 minutes. Then let it cool down at room temperature and extrude the sericin solution. Obtain the sericin solution that has been treated in hot water at 80°C, 90°C or 100°C for 0h in a water bath, and filter it with gauze to remove impurities. Take a small amount of sericin solution for SDS-PAGE electrophoresis analysis to determine the molecular weight of sericin. The rest of the solution was pre-frozen in a refrigerator at -20°C for 3 hours, then freeze-dried in a freeze dryer at -50°C for 24 hours, then developed into powder and placed in a desiccator for storage.

[0017] Use an analytical balance to accurately weigh 300 mg of sericin powder and put it into a precisely weighed dry centrifuge tube (W 1 ), and add a certain amount of distil...

Embodiment 2

[0021] Take several dry cocoon layers, remove dirt and cut into pieces, weigh a certain amount of broken cocoon pieces, put them into a beaker containing 30 times the volume (V / W) of boiling distilled water, and heat and boil for 30 minutes. Then let it cool down at room temperature and extrude the sericin solution. Then, the obtained sericin solution was placed in a water bath of 80°C hot water for 6 hours and then cooled, and filtered with gauze to remove impurities to obtain a 80°C hot water bath of 6 hours sericin solution. Take a small amount of sericin solution for SDS-PAGE electrophoresis analysis to determine the molecular weight of sericin. The rest of the solution was pre-frozen in a refrigerator at -20°C for 3 hours, then freeze-dried in a freeze dryer at -50°C for 24 hours, then developed into powder and placed in a desiccator for storage.

[0022] Use an analytical balance to accurately weigh 300 mg of sericin powder, put it into an accurately weighed dry centrifuge t...

Embodiment 3

[0025] Take several dry cocoon layers, remove dirt and cut into pieces, weigh a certain amount of broken cocoon pieces, put them into a beaker containing 30 times the volume (V / W) of boiling distilled water, and heat and boil for 30 minutes. Then let it cool down at room temperature and extrude the sericin solution. Then, the obtained sericin solution was placed in a water bath of 80°C hot water for 12 hours, and then cooled, and filtered with gauze to remove impurities to obtain a 80°C hot water bath of 12 hours sericin solution. Take a small amount of sericin solution for SDS-PAGE electrophoresis analysis to determine the molecular weight of sericin. The rest of the solution was pre-frozen in a refrigerator at -20°C for 3 hours, then freeze-dried in a freeze dryer at -50°C for 24 hours, then developed into powder and placed in a desiccator for storage.

[0026] Use an analytical balance to accurately weigh 300 mg of sericin powder, put it into an accurately weighed dry centrifug...

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Abstract

The invention discloses a preparation method of water-soluble silk glue with different molecular weights, which comprises the following steps: (1) cocoon shells are taken and put into boiled distilled water to be degummed, the bath ratio is 1:30, and the degumming time is 30 minutes; (2) the obtained degumming liquid carries out water bath processing for 0-12 hours in heat water with the temperature ranging from 80 DEG C to 100 DEG C; and (3) silk glue liquid prepared after the water bath processing of the step (2) is frozen and dried so as to obtain the water-soluble silk glue with different molecular weights. The water-soluble silk glue with different molecular weights is prepared by a hot water processing method without adding any chemical substance, thereby the invention meets the requirement of environment protection and also has moderate silk glue preparation condition, simple preparation technology and low equipment requirement; and in addition, the molecular weight distribution of the water-soluble silk glue is different from silk glues prepared by other methods, thereby the invention provides a new foundation for the utilization of the water-soluble silk glue.

Description

Technical field [0001] The invention relates to the processing and utilization field of silk sericin, in particular to a method for preparing cocoon layer sericin into water-soluble sericin of different molecular weights by hot water treatment. Background technique [0002] As a natural biopolymer material, silk sericin has a variety of excellent properties such as reactivity, biocompatibility and biodegradability, and it is easy to be made into solutions, powders, membranes, gels and fibers in various forms. It has broad application prospects in the fields of industry, agriculture, medicine, nutrition and beauty. The molecular weight of sericin has a greater impact on its application range. For example, high molecular weight sericin is suitable for the development of functional biofilms, biomedical materials, elastic gels and functional fibers, while low molecular weight sericin is suitable Used in the preparation of cosmetics, food and medicines. Therefore, sericin with differe...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 朱良均张海萍周林巨闵思佳
Owner ZHEJIANG UNIV
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