Preparation method of water-soluble silk glue with different molecular weights
A water-soluble, molecular weight technology, applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of increasing operational complexity and cost, impact and environmental pollution, and achieve environmental protection, low equipment requirements, Simple preparation process
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Embodiment 1
[0016] Take several dry cocoon layers, remove the stolen goods and cut into pieces, weigh a certain amount of broken cocoon pieces, put them into a beaker containing 30 times the volume (V / W) of boiling distilled water, and heat and boil for 30 minutes. Then let it cool down at room temperature and extrude the sericin solution. Obtain the sericin solution that has been treated in hot water at 80°C, 90°C or 100°C for 0h in a water bath, and filter it with gauze to remove impurities. Take a small amount of sericin solution for SDS-PAGE electrophoresis analysis to determine the molecular weight of sericin. The rest of the solution was pre-frozen in a refrigerator at -20°C for 3 hours, then freeze-dried in a freeze dryer at -50°C for 24 hours, then developed into powder and placed in a desiccator for storage.
[0017] Use an analytical balance to accurately weigh 300 mg of sericin powder and put it into a precisely weighed dry centrifuge tube (W 1 ), and add a certain amount of distil...
Embodiment 2
[0021] Take several dry cocoon layers, remove dirt and cut into pieces, weigh a certain amount of broken cocoon pieces, put them into a beaker containing 30 times the volume (V / W) of boiling distilled water, and heat and boil for 30 minutes. Then let it cool down at room temperature and extrude the sericin solution. Then, the obtained sericin solution was placed in a water bath of 80°C hot water for 6 hours and then cooled, and filtered with gauze to remove impurities to obtain a 80°C hot water bath of 6 hours sericin solution. Take a small amount of sericin solution for SDS-PAGE electrophoresis analysis to determine the molecular weight of sericin. The rest of the solution was pre-frozen in a refrigerator at -20°C for 3 hours, then freeze-dried in a freeze dryer at -50°C for 24 hours, then developed into powder and placed in a desiccator for storage.
[0022] Use an analytical balance to accurately weigh 300 mg of sericin powder, put it into an accurately weighed dry centrifuge t...
Embodiment 3
[0025] Take several dry cocoon layers, remove dirt and cut into pieces, weigh a certain amount of broken cocoon pieces, put them into a beaker containing 30 times the volume (V / W) of boiling distilled water, and heat and boil for 30 minutes. Then let it cool down at room temperature and extrude the sericin solution. Then, the obtained sericin solution was placed in a water bath of 80°C hot water for 12 hours, and then cooled, and filtered with gauze to remove impurities to obtain a 80°C hot water bath of 12 hours sericin solution. Take a small amount of sericin solution for SDS-PAGE electrophoresis analysis to determine the molecular weight of sericin. The rest of the solution was pre-frozen in a refrigerator at -20°C for 3 hours, then freeze-dried in a freeze dryer at -50°C for 24 hours, then developed into powder and placed in a desiccator for storage.
[0026] Use an analytical balance to accurately weigh 300 mg of sericin powder, put it into an accurately weighed dry centrifug...
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