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Breeding method of pyemotes zhonghuajia and application thereof in pest biological control

A technology of Chinese beetle and cataclysia, applied in the field of biological control, can solve the problems of increasing pests in the environment, high production cost, and inability to supply yearly, and achieve the effect of maintaining the original species and low cost

Active Publication Date: 2011-11-16
CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is a large-scale breeding method in China, but the species of mites used are all Pyemotes scolyti, that is, Pyemotes scolyti, which belongs to the Pyemotes scolytigroup. Insects affect the effect of artificial utilization. At the same time, several alternative hosts adopted by the method have shortcomings: some alternative host sources cannot be guaranteed. For example, using the larvae of P. Larvae, this is not only affected by natural conditions, but also the quantity of Alpinia bichaecens provided by nature cannot meet the requirements at all; using the larvae of Shuangtiaoshan longhorn beetle to breed cat mites, the source of natural beetle beetle larvae is very small, which cannot meet the scale The needs of artificial breeding of cat mites can only be solved by artificial breeding
Some alternative host sources are affected by the market, such as silkworm chrysalis or bee larvae, which have high production costs or cannot be supplied year-round, such as grubs, and raising grubs in the field will increase pests in the environment and cause farmers to lose fruit trees and crops
In particular, the above-mentioned alternative hosts are all raised by naked worms, which is not convenient for long-distance transportation
[0005] The Chinese beetle acarina bred by the present invention is a newly discovered species in my country, and there is no relevant report at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Breeding of the original species of the Chinese beetle cat mite

[0035]The mature larvae (or early instar pupae) of the wild Chinese beetle and the wild bark beetle collected from the adult body of the persimmon tree beetle were placed in a finger tube feeder (the specification is long 7cm, inner diameter 2cm glass tube), at 27±2°C, relative humidity 50%-80%, well-ventilated, clean and dust-free environment, inoculate, plug the tube mouth with cotton plug, cultivate for 7 days, and breed out The offspring of the Chinese beetle C. chinensis were used as the original species of C. sinensis.

Embodiment 2

[0036] Embodiment 2 Utilizing the larvae of the greater mellonella moth as an alternative host to produce the original species of the Chinese beetle

[0037] In this example, the artificially reared larvae of Melonella mellonella were selected as an alternative host for the production of the original species of the Chinese beetle. Select the 3-4 instar larvae of Mellonella mellonella, put them in a petri dish, pull out the cotton plug of the glass finger tube containing the original species of the cat mite, and place it in a petri dish at a temperature of 27±2°C, relatively Under the condition of 50%-80% humidity, the original seed and the substitute host were placed in a glass petri dish feeder (a glass petri dish with a diameter of 15 cm) at a ratio of 1:3, and after 8 hours of inoculation, they were transferred to a finger tube feeder. Breeding for 7 days until new Chinese beetle mite is produced can be used as the original species of Chinese beetle mite production.

Embodiment 3

[0038] Embodiment 3 Utilize tobacco beetle as alternative host to produce the original species of Chinese beetle

[0039] Tobacco beetle early instar pupae were selected as an alternative host for the production of the original species of the Chinese beetle, and the alternative host, the tobacco beetle pupae, was placed in a petri dish, and the cotton plug of the glass finger tube containing the original species of the Chinese beetle was pulled out at the same time , also placed in the same petri dish, at 27±2°C, relative humidity 70%-80%, well-ventilated, clean and dust-free environment, the original species and the substitute host were placed in a glass petri dish feeder according to the ratio of 1: 5, after 8 hours of inoculation, transfer it to a petri dish feeder (a glass petri dish with a diameter of 15 cm), and raise it for 7 days until the new Chinese beetle P.

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PUM

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Abstract

The invention relates to a breeding method of pyemotes zhonghuajia, comprising wild pyemotes zhonghuajia collected from persimmon smotylon japonicam lesne imago or stenhomalus taiwanus matsushita larva is propagated by using scolytus seulensis musayawa larva or pupa, stenhomalus taiwanus matsushita larva or early age of pupa as breeder seed; under the proper condition, greater wax moth larva, lasioderma serricorne larva, the early age of pupa, early age of pupa of martianus dermestoides chevrolat or gelechiid can be taken as substitutional host, and the breeder seed is inoculated into the substitutional host as stock seed; the gelechiid, sitophilus zeamais, rice weevil larva, the early age of pupa or the greater wax moth larva can be taken as substitutional host, and the stock seed and thesubstitutional host can be inoculated according to the proportion of 1:3; under the proper condition, the pyemotes zhonghuajia can be cultured and bred. The method has the advantages of simple operation, low production cost, being suitable for large scale batch production and long-distance transportation and the like, and the bred pyemotes zhonghuajia can be applied to the biological control of disguised and boring pests.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a breeding method of the Chinese beetle and its application in biological control of pests. Background technique [0002] Boring pests are destructive pests of forest and fruit, and their hidden habits bring great difficulties to control, not only causing great economic losses to forestry, but also causing the deterioration of ecological environment. At present, the control of borer pests mainly depends on chemical control and artificial control. Chemical control is easy to cause environmental pollution, but it is difficult to be effective, and many natural enemy insects are completely destroyed, causing secondary pests to rise to the main pests, causing secondary pests. Rampant occurrence of pests. The efficiency of artificial control is low, and it is easy to cause artificial damage to the tree body. [0003] Scholars at home and abroad have long been l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01M99/00
Inventor 于丽辰贺丽敏焦蕊许长新郝宝锋韩继成
Owner CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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