Breeding method of pyemotes zhonghuajia and application thereof in pest biological control
A technology for beetles and cat mites, which is applied in the field of biological control, can solve the problems of increasing pests in the environment, high production costs, and the number of Alpinia cerevisiae cannot meet the requirements, and achieve the effect of maintaining the original species and low cost
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Embodiment 1
[0034] Embodiment 1 Breeding of the original species of the Chinese beetle pleura mite
[0035]The mature larvae (or early instar pupae) of the wild Chinese beetle and the wild bark beetle collected from the adult body of the persimmon tree beetle were placed in a finger tube feeder (the specification is long 7cm, inner diameter 2cm glass tube), at 27±2°C, relative humidity 50%-80%, well-ventilated, clean and dust-free environment, inoculate, plug the tube mouth with cotton plug, cultivate for 7 days, and breed out The offspring of the Chinese beetle C. chinensis were used as the original species of C. sinensis.
Embodiment 2
[0036] Embodiment 2 Utilizing the larvae of the greater mellonella moth as an alternative host to produce the original species of the Chinese beetle
[0037] In this example, the artificially reared larvae of Melonella mellonella were selected as an alternative host for the production of the original species of the Chinese beetle. Select the 3-4 instar larvae of Mellonella mellonella, put them in a petri dish, pull out the cotton plug of the glass finger tube containing the original species of the cat mite, and place it in a petri dish at a temperature of 27±2°C, relatively Under the condition of 50%-80% humidity, the original seed and the substitute host were placed in a glass petri dish feeder (a glass petri dish with a diameter of 15 cm) at a ratio of 1:3, and after 8 hours of inoculation, they were transferred to a finger tube feeder. Breeding for 7 days until new Chinese beetle mite is produced can be used as the original species of Chinese beetle mite production.
Embodiment 3
[0038] Embodiment 3 Utilize tobacco beetle as alternative host to produce the original species of Chinese beetle
[0039] Tobacco beetle early instar pupae were selected as an alternative host for the production of the original species of the Chinese beetle, and the alternative host, the tobacco beetle pupae, was placed in a petri dish, and the cotton plug of the glass finger tube containing the original species of the Chinese beetle was pulled out at the same time , also placed in the same petri dish, at 27±2°C, relative humidity 70%-80%, well-ventilated, clean and dust-free environment, the original species and the substitute host were placed in a glass petri dish feeder according to the ratio of 1: 5, after 8 hours of inoculation, transfer it to a petri dish feeder (a glass petri dish with a diameter of 15 cm), and raise it for 7 days until the new Chinese beetle P.
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