Method for detecting potential inherent toxicity of organic pollutants in water body

A technology for organic pollutants and genotoxicity, which is used in the field of detecting the potential genotoxicity of organic pollutants in water bodies to achieve the effect of solving vulnerable

Inactive Publication Date: 2009-11-04
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the comet test on algae has not been reported in China, and there are few reports abroad.

Method used

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  • Method for detecting potential inherent toxicity of organic pollutants in water body
  • Method for detecting potential inherent toxicity of organic pollutants in water body
  • Method for detecting potential inherent toxicity of organic pollutants in water body

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1 Pretreatment of water samples:

[0042] In June 2008, 20L of water samples were collected at the water source of Meiliang Bay in Taihu Lake (Wuxi) according to the standard method of the National Environmental Protection Agency. After standing still for 24 hours, the suspended particles were removed by filtration and enriched by XAD-2 resin (Sigma) adsorption column. The flow rate is 30-40mL / min, and then nitrogen is used to remove water, and methanol, acetone and dichloromethane are used to elute in sequence. The eluate is concentrated at 50°C, dried, and finally dissolved in dimethylsulfoxide (DMSO). Dilute to 2.0mL, and store at -18°C in the dark for later use.

[0043] 2 Culture of Euglena gracilis:

[0044] Euglena gracilis was provided by the Freshwater Algal Species Bank (FACHB) of the Type Culture Collection Committee of the Institute of Hydrobiology, Chinese Academy of Sciences, and cultured on Checcucci (1976) medium. The Meiliang Bay water in Taihu Lake ...

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Abstract

The invention discloses a method for detecting potential inherent toxicity of organic pollutants in a water body, which comprises the following steps: using resin to perform enrichment of water samples first; and applying a comet assay of Euglena gracilis to research the inherent toxicity of the organic pollutants in the water body. The key technique lies in the innovation of glue manufacturing process, namely adopting fractioning at a first layer, then adopting the conventional 'sandwich' glue manufacturing method, and manufacturing the glue by using a normal melting point glue with a higher melting point at a third layer, thus the defect that a rubber plate in the conventional method is easy to damage is overcome. By performing optimization research on cracking time and electrophoresis conditions of algae comets, a perfect image result is obtained. At present, the method for detecting the potential inherent toxicity of the organic pollutants in the water body through algae single cell gel electrophoresis (SCGE) is not reported, and algae are applied to the SCGE to widen the research objects of the SCGE.

Description

technical field [0001] The invention belongs to the field of environmental health genetic toxicology, and in particular relates to a method for detecting the potential genetic toxicity of organic pollutants in water bodies. Background technique [0002] Scholars at home and abroad have successively discovered the existence of mutagenic organic pollutants in tap water and source water, and relevant epidemiological survey results have also shown that organic pollution in drinking water is related to some human cancers. Therefore, it is imminent to investigate the harmful organic pollutants in water and study their genotoxicity. Some people even propose that whether the source water contains genotoxic substances should be the main index for evaluating water quality. Therefore, establishing an effective test method to evaluate the genotoxicity of organic matter in water is an important task in environmental monitoring. [0003] The biological detection of genotoxic effects of v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64G01N27/447C12R1/89
Inventor 崔益斌李梅高香玉胡长伟孔志明方昊程树培
Owner NANJING UNIV
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