Wettable powder of high sporopollen of trichoderma and preparation method thereof

A technology of wettable powder and Trichoderma, applied in the field of Trichoderma live biological preparations and its preparation, to achieve excellent product performance indicators

Active Publication Date: 2009-11-11
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except for a few effective extracts of Trichoderma, most of these preparations use live organisms, including mycelia, conidia, a

Method used

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  • Wettable powder of high sporopollen of trichoderma and preparation method thereof
  • Wettable powder of high sporopollen of trichoderma and preparation method thereof
  • Wettable powder of high sporopollen of trichoderma and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] ——Biocompatibility test in preparation

[0033] 1. Materials and methods

[0034] 1.1 Materials

[0035] Diatomite, bentonite, talcum powder, and light calcium carbonate are four kinds of adsorbents. The synergist is the original drug powder of dexazone used in the Trichoderma semi-selective medium, and its dosage is mainly screened.

[0036] 1.2 Method

[0037] 1.2.1 Screening of adsorbents

[0038] The effect on the germination of conidia: Mix all kinds of adsorbents to be tested with Trichoderma sporopollen in a ratio of 9:1, place them in an incubator at 26-28°C for 5 days, and use Trichoderma semi-selective culture medium TSM [MgSO 4 ·7H 2 O 0.2g; K 2 HPO 4 0.9g; KCl 0.25g; NH 4 NO 3 1.0g; glucose 3.0g; chloramphenicol 0.25g; 2 O 1000ml. ] 26 ~ 28 ℃, 150r / m shaking culture for 24h, hemocytometer board to measure the spore germination rate, with Trichoderma sporopollen as a control.

[0039] Effects on mycelial growth and sporulation: Add various adso...

Embodiment 2

[0054] With 80,000,000,000 / gram, living spore rate more than 90% Trichoderma high sporopollen 5Kg, the former powder of Dike Song 2Kg, sodium lignosulfonate 3Kg, gac 1Kg, citric acid 0.5Kg, sodium carboxymethyl cellulose 2Kg, add into 86.5Kg of diatomite, fully mixed in a mixer, pre-crushed vertically, and then crushed by airflow, passed through a 325-mesh standard sieve, and inspected for quality. % or more, wettability of less than 2 minutes, suspension rate of more than 70%, fineness (through 325-mesh standard sieve) of more than 99%, moisture content of less than 2%, pH value of 5-6, etc., are qualified Trichoderma spp. powder wettable powder.

Embodiment 3

[0056] Trichoderma high sporopollen 6Kg with 60 billion pieces / g and living spore rate above 90%, Dikesong raw powder 1.5Kg, sodium lignosulfonate 2.5Kg, activated carbon 1Kg, citric acid 0.5Kg, sodium carboxymethyl cellulose 1.5 Kg, added to 87Kg bentonite, fully mixed in the mixer, pre-crushed vertically, and then crushed by airflow, passed through a 325-mesh standard sieve, quality inspection, to meet the content of Trichoderma spores of more than 500 million per gram, and the live spore rate of 80 % or more, wettability of less than 2 minutes, suspension rate of more than 70%, fineness (through 325-mesh standard sieve) of more than 99%, moisture content of less than 2%, pH value of 5-6, etc., are qualified Trichoderma spp. powder wettable powder.

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Abstract

The invention discloses a wettable powder of high sporopollen of trichoderma and a preparation method thereof. The wettable powder comprises the following materials with mass percentages of: 5 to 6 percent of high sporopollen of trichoderma, 1 to 2 percent of fenaminosulf, 85 to 90 percent of diatomite and bentonite, 2 to 3 percent of sodium lignosulphonate, 0.5 to 1.0 percent of active carbon, 0.1 to 0.5 percent of citric acid and 1 to 2 percent of sodium carboxymethyl cellulose, wherein the classified name of the biocontrol strain adopted by the high sporopollen of trichoderma is TV-1 (trichoderna viride) and the preserving number is CGMCC No.2848. All the materials are mixed evenly, pre-crushed, then crushed by air flow, and sieved by a standard sieve with the meshes of 325; and by quality inspection, the qualified wettable powder of high sporopollen of trichoderma meets the following conditions that the content of the trichodrema spore is more than 0.5 billion/g, active spore rate is more than 80 percent, wettability is less than two minutes, suspension percentage is more than 70 percent, fineness (sieved by the standard sieve with the meshes of 325) is more than 99 percent, the water content is less than 2 percent, pH is 5 to 6 and the like.

Description

technical field [0001] The present invention relates to biopesticide, more specifically, the present invention relates to a kind of Trichoderma live biological agent and preparation method thereof Background technique [0002] As a class of biocontrol filamentous fungi, Trichoderma has been used in the biological control of soil-borne fungal diseases as early as the 1930s. In recent decades, with the wide application of greenhouse cultivation technology, Trichoderma has gradually been used for the biological control of some stubborn leaf diseases, such as gray mold, powdery mildew, and leaf mold. The antagonism of Trichoderma to plant pathogenic fungi is mainly manifested in the production of antibiotics, heavy parasitism, bacteriolysis, and competition. Among them, the competition and heavy parasitism of Trichoderma play an important role in the process of inhibiting pathogenic fungi. [0003] The preparation of Trichoderma is currently mainly wheat bran powder. In recent...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N51/00A01N25/14A01P3/00
Inventor 方敦煌王革邓云龙顾金刚邓建华宋春满
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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