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Method for quantifying total individual number of ulva algae at microscopic stage in seawater

A seawater and Ulva technology, applied in the field of prediction of Ulva green tide seaweed outbreaks, can solve the problems of indistinguishable, unreliable methods, and inability to accurately estimate the biomass of Enteromorpha, so as to overcome the induction effect and achieve reliable morphological classification Effect

Inactive Publication Date: 2012-03-28
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual coastal and offshore investigations, there is no effective technical method to accurately quantify the number of Ulva algae individuals in seawater, so it is impossible to accurately estimate the potential Enteromorpha biomass in seawater
The suction filtration method is to filter seawater onto a 0.45 μm or 0.22 μm filter membrane, and the method of counting the number of gametes or spores in seawater is unreliable, because the gametes and spores of Ulva genus seaweed are difficult to distinguish from many single-celled seaweeds; at the same time Since the initial development stages of different Ulva species are difficult to distinguish, the results obtained cannot be counted from the Ulva species

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] On February 20, 2009, 5L seawater samples were obtained from the ferry terminal, Tuandao Bay, Zhanqiao, No. 1 Bathing Beach, May Fourth Square and Shilaoren on the coast of Qingdao, and the visible algae in the water were filtered through 100-mesh gauze and tulle , transferred to a 1L beaker (n=5), adding 500 μL of saturated germanium dioxide aqueous solution to inhibit the growth of diatoms, and then adding it to f / 2 nutrient salts (1 mL each of mother liquor A and B) for cultivation; Then turn to a temperature of 15±0.5°C and a light intensity of 80±5μmol photons m -2 the s -1 1. The photoperiod is 12 hours during the day: 12 hours of dark night conditions, inflated air culture, after 7 days of culture, change the f / 2 culture medium prepared by freshly sterilized seawater for the first time, and then change the culture medium every 5 days, culture for 3-4 weeks, and count stones For the total number of seaweeds of the genus Brasenia, Zhanqiao has the most seedlings, ...

Embodiment 2

[0021] On April 7, 2009, it was obtained from a total of 6 stations including the Xishu full-floating laver breeding area, the Liuxu semi-floating laver breeding area and shrimp pond, and the Xiangshui full-floating laver breeding area and aquatic animal breeding pond on the coast of Lianyungang, Jiangsu. For 5L sea water sample, filter out the visible algae in the water through a 100-mesh gauze screen, transfer it to a 1L beaker (n=5), add 500 μL of saturated germanium dioxide aqueous solution to inhibit the growth of diatoms, and then add f / 2 Nutrient salts (1mL each of mother liquor A and B), and then transferred to a temperature of 15±0.5°C and a light intensity of 80±5μmol photons m -2 the s -1 1. The photoperiod is 12 hours during the day: 12 hours of inflated (air) culture under dark conditions. After 7 days of cultivation, the f / 2 culture solution prepared by freshly sterilized seawater is replaced for the first time. After that, the culture solution is changed every 5...

Embodiment 3

[0023] On April 17, 2009, 5L seawater samples were obtained from the ferry terminal, Tuandao Bay, Zhanqiao, No. 1 Bathing Beach, May Fourth Square and Shilaoren on the coast of Qingdao, and the visible algae in the water were filtered through 100-mesh gauze and tulle , transferred to a 1L beaker (n=5), added 500 μL of saturated germanium dioxide aqueous solution to the 1L water sample, and then added f / 2 nutrient salt (1 mL each of mother liquor A and B), and then transferred to a temperature of 15±0.5 °C , the light intensity is 80±5μmol photons m -2 the s -1 1. The photoperiod is 12h during the day: 12h night under aerated (air) culture. After 7 days of culture, replace the f / 2 culture medium prepared by freshly sterilized seawater for the first time, and then change the culture medium every 5 days, and cultivate for about 3-4 weeks. According to the statistics of the total number of Ulva seaweed, Zhanqiao has the most seedlings, with an average of 2060 plants per liter; th...

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Abstract

The invention relates to a method for predicting breakout of ulva green algae, in particular to a method for accurately quantifying total individual number of ulva algae at a microscopic stage in seawater. The method comprises the following steps: filtering a seawater sample by using a silk gauze of 100 meshes, adding 500mu L saturated germanium dioxide water solution into 1 liter of the filtered seawater sample, and then adding f / 2 nutrient salt into the seawater sample to culture the seawater by charging air; culturing the seawater added with the f / 2 nutrient salt by charging the air under the conditions of temperature of between 15 and 20 DEG C, light intensity of 50 to 100mu mol photons m<-2>s<-1> and light period of 12 hours at daytime and 12 hours at night, changing fresh f / 2 culture solution after the seawater is cultured for 7 days, later changing the culture solution every five days, and culturing the sweater for 3 to 4 weeks for standby; and determining the total individual number of the ulva algae in each liter of the seawater after the seawater is cultured. The method eliminates death caused by natural external force, mechanical damage and the like or reduced number caused by the predation of natural enemies using the ulva algae as food under the nature condition.

Description

technical field [0001] The invention relates to a method for predicting the outbreak of Ulva green tide seaweed, in particular to a method for accurately quantifying the total individual number of Ulva (including Enteromorpha) seaweed microscopic stages in seawater. Background technique [0002] The outbreak of green tide is the result of the combined effects of the special growth and reproduction characteristics of Enteromorpha, marine climate environment and unique coastal aquaculture habits. Therefore, in-depth research on each aspect will play a positive role in evaluating the green tide outbreak. Among the biological aspects, how to accurately quantify the number of Ulva algae individuals in seawater is particularly important. In the actual coastal and offshore investigations, there is no effective technical method to accurately quantify the individual number of Ulva genus seaweed in seawater, so it is impossible to accurately estimate the potential Enteromorpha biomas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84A01G33/00
CPCY02A40/80
Inventor 逄少军刘峰单体锋徐娜张玉荣高素芹张志怀
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI