Method for preventing and controlling tissue culture seedling pollution

Abstract

The invention relates to a method for preventing and controlling tissue culture seedling pollution by mercuric chloride. In the method for preventing and controlling tissue culture seedling pollution, the pollution of a polluted tissue culture seedling can be eliminated by a plurality of transfer periods, wherein each transfer period comprise the following two steps: (1) adding mercuric chloride into a culture medium of the tissue culture seedling; and (2) transferring the polluted tissue culture seedling on the culture medium containing the mercuric chloride. The mercuric chloride adopted by the invention is spectrum fungicide, has the effects of inhibition and sterilization on the polluted tissue culture seedling culture medium, has less dosage, can be directly added into the culture medium, carries out high pressure sterilization along with the culture medium and does not reduce the sterilizing effect; the pollution rate is reduced below 5 percent, the production cost can be effectively reduced, and economic benefits are improved; and as for some tissue culture seedlings rushing for a growing season, when the seedlings for production are polluted, the invention can eliminate the pollution, supplement the seedling dosage and reduce loss. In addition, the used culture medium carries out chemical processing so as to prevent pollution and be beneficial to environmental protection.

Description

technical field [0001] The invention relates to a method for preventing and controlling the pollution of tissue culture seedlings in plant tissue culture, in particular to a method for preventing and controlling the pollution of tissue culture seedlings by using mercuric chloride. Background technique [0002] The industrialized production of tissue cultured seedlings has become an emerging industry with huge market potential, and is one of the important means of industrialized seedling raising technology for famous and high-quality plants. The adjustment of the physical and chemical environment can be planned in a short period of time to cultivate multiple excellent varieties with the same genetic characteristics. However, the pollution that occurs during the production of tissue cultured seedlings has led to an increase in production costs and has limited the rapid development of industrialized production of tissue cultured seedlings. [0003] Contamination in plant tissu...

AI Technical Summary

Problems solved by technology

Once the tissue culture materials are contaminated, it is difficult to rescue. The general method is to discard them and do another one, which wastes a lot of manpower, material and financial resources, especially for the preservation of some precious materials, causing irreparable losses. For seedling cultivation, it will delay production and cause serious losses

[0004] At present, the research on pollution control is mostly biased towards the control and physical prevention of antibiotics. Antibiotics have a certain degree of pertinence for pollution prevention and control, but procedures such as filtration and addition are required, and the steps are cumbersome; if the antibiotics are autoclaved with the medium, the sterilization The effect will decrease again, and the prevention and control effect is not ideal

The pollution caused by the mixture of bacteria and yeast, although there are many reports on the treatment of antibiotics and other bactericides, but so far no antibiotics have been found to be effective against all kinds of bacteria, so the control effect is not ideal. The loss caused is immeasurable and irreparable