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Biphenyl compound, preparation method and uses thereof

A compound, biphenyl technology, applied in the field of biphenyl compounds and their preparation, can solve problems such as drugs that have not yet been seen

Active Publication Date: 2018-03-16
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical structure and antibacterial activity of this compound have not yet been reported, so there are no related drugs on the market

Method used

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  • Biphenyl compound, preparation method and uses thereof
  • Biphenyl compound, preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A biphenyl compound, the structural formula of the biphenyl compound is as shown in I:

[0024] (I).

Embodiment 2

[0026] The preparation method of the biphenyl compound shown in formula I specifically comprises the steps:

[0027] (1) Fermentation production

[0028] The Aspergillus versicolor with the preservation number 3A00080 was revived by streaking, inoculated on the PDA solid medium, and cultured in a 28°C incubator for 3 days; the inoculum of Aspergillus versicolor was inoculated at 5% by volume in a 400 mL culture medium. (PDB liquid medium) in a 1000mL Erlenmeyer flask, cultured statically at 28°C for 15 days to obtain mycelium and fermentation broth; the preparation method of the PDA solid medium was to mix 200 grams of potatoes, 20 grams of glucose and 15 Add ~20g of agar to 1L of seawater; the preparation method of PDB liquid medium is to add 10g of potatoes and 20g of glucose into 1L of seawater, and adjust the pH to 7.0;

[0029] (2) Obtaining the extract

[0030] Extract the mycelium and fermentation broth obtained in step (1) three times with an equal volume of ethyl ac...

Embodiment 3

[0039] Test of compound I antibacterial activity in vitro

[0040] 1. Experimental samples and experimental methods

[0041] Preparation of the test sample solution The test sample is the pure compound I isolated and refined in the above-mentioned Example 1 or Example 2. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement. The test strain was Bacillus subtilis Bacillus subtilis, Staphylococcus aureus Staphylococcus aureus and Saccharomyces cerevisiae Saccharomyces cerevisiae. All strains were cultured in LB medium in a 37°C incubator.

[0042] 2. Antibacterial activity test method

[0043] Filter paper disc method: Prepare a certain number of circular filter paper discs (5-6 mm in diameter) and sterilize them at 120°C. Then put the filter paper on a sterile glass plate, take 10 μL of the compound solution to absorb on the filter paper, and absorb equal volumes of chlorampheni...

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Abstract

The invention discloses a biphenyl compound, a preparation method and uses thereof. The preparation method comprises: carrying out fermentation culture on Aspergillus versicolor with a preservation number of 3A00080 to obtain a fermentation broth containing a biphenyl compound and hypha, extracting the fermentation broth by using ethyl acetate to obtain a crude extract, carrying out pressure reducing silica gel column chromatography on the crude extract, carrying out reverse phase medium pressure column chromatography, and carrying out separating purification through reverse phase semi-preparative high performance liquid chromatography to obtain the biphenyl compound. According to the present invention, the biphenyl compound can inhibit Saccharomyces cerevisiae, and can strongly inhibit Bacillus subtilis and Staphylococcus aureus.

Description

technical field [0001] The invention relates to a biphenyl compound, in particular to a biphenyl compound and a preparation method and use thereof. Background technique [0002] Biphenyl compounds widely exist in many natural products with physiological activities, and their synthesis has attracted more and more attention. Biphenyl compounds exhibit diverse structures and rich activity characteristics. However, most biphenyl compounds are prepared through organic synthesis, and very few are plant or prokaryotic sources. The inventors have learned from studies that the liquid fermentation product of Aspergillus versicolor (preservation number: MCCC 3A00080) extracted with ethyl acetate has good antibacterial activity, and then studied its active components. The chemical structure and antibacterial activity of this compound have not been reported at present, so there is no drug related to it in the market. Contents of the invention [0003] The technical problem to be sol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/23C07C41/34A61P31/04A61P31/10A01P3/00A01P1/00C12P7/22C12R1/66
CPCC07C41/34C07C43/23C12P7/22
Inventor 丁立建何山朱静轩周珂欣斯拉瓦·爱泼斯坦
Owner NINGBO UNIV
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