Primers, probes and kits for detection of coxsackie virus a16 nucleic acid

A Coxsackie virus, A16 technology, applied in the field of probes, kits, and primers for Coxsackie virus A16 nucleic acid detection, can solve the problems of cumbersome steps, long detection period, easy to miss detection, etc., and achieve high accuracy , high sensitivity, good primer specificity
CN101676406BInactive Publication Date: 2012-02-01ไฝ•้›…้’ +1
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
ไฝ•้›…้’
Publication Date
2012-02-01
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The present invention relates to a primer, a probe and a kit for detecting Coxsackievirus A16 nucleic acid. The nucleotide sequence of the primer is: forward primer 5'-GAACCATCACTCCACACAGGAG-3'; reverse primer 5'-GTACCCGTGGTGGGCATTG-3'. The nucleotide of the probe is 5'-CAGCCATTGGGAATTTCTTTAGCCGTG-3'. Also provided is a method for detecting Coxsackievirus A16 nucleic acid. The method uses sample RNA as a template, uses the above primers and probes to perform real-time fluorescent RT-PCR amplification, and judges the result according to the amplification curve after each cycle. The invention has good primer specificity, fast and simple detection method, high accuracy and high sensitivity, and provides scientific basis for etiological diagnosis and differential diagnosis of hand, foot and mouth disease and related diseases.
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Description

technical field

[0001] The invention relates to biological detection technology, in particular to primers, probes and kits for coxsackievirus A16 nucleic acid detection. Background technique

[0002] Coxsackievirus A16 (Coxsackievirus A16, CA16 for short) is a single-stranded positive-sense RNA virus belonging to the Picoranviridae family. CA16 is an important pathogen that causes hand, foot and mouth disease in children. The clinical symptoms caused by it are difficult to distinguish from hand, foot and mouth disease caused by enterovirus 71 infection. However, under normal circumstances, CA16 infection generally does not cause serious neurological related diseases. , is often overlooked due to its subclinical infection. Many countries and regions in the world have reported the prevalence of hand, foot and mouth disease caused by CA16, and there is a pandemic every 3-5 years. In recent years, CA16 has been paid more and more attention due to its association with myocardit...

Claims

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