Methods for producing fermentation products

一种发酵产物、生物体的技术，应用在发酵、生物燃料等方向，能够解决耐受性限制、除去毒素困难、耗时且昂贵等问题

Inactive Publication Date: 2010-03-24
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermentation cycles can typically run for as many as 96 hours or longer, making each cycle time-consuming and expensive
[0005] The process of producing fermentation products from lignocellulosic-containing material or cellulosic "biomass" is also limited by the tolerance of the fermenting organisms to many of the toxins found in the crude hydrolysates used in the fermentation process
Removing toxins from hydrolysates is difficult, time-consuming and expensive

Method used

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  • Methods for producing fermentation products
  • Methods for producing fermentation products
  • Methods for producing fermentation products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0374] Ethanol production for high cell counts

[0375] The effect of high yeast pitch (cell count) and cell recirculation on ethanol production was tested by inoculating filtered pretreated corn stover (PCS) enzymatic hydrolyzate at various starting yeast cell concentrations. At 24 and 48 hours after the start of the fermentation, the cells were recirculated by centrifugation, the spent hydrolyzate was removed, and fresh hydrolyzate was added.

[0376] method:

[0377] Unwashed PCS (acid-catalyzed, steam-exploded, The National Renewable Energy Laboratory, Golden , CO) hydrolyzed for 72 hours. After hydrolysis, the slurry was centrifuged at 3000 rpm for 10 minutes and the supernatant was collected by filtration using a 0.45 micron Whatman filter.

[0378] Varying amounts of RED STAR ranging from 1 g stem cells / L to 50 g stem cells / L were added to wells of 24-well cell culture plates (Whatman International Ltd., Florham Park, NJ) TM yeast. Add 4 mL of PCS Enzymatic Hyd...

Embodiment 2

[0383] Ethanol production at low cell counts

[0384] The effect of various amounts of sugar solution and filtered pretreated corn stover (PCS) enzymatic hydrolyzate on ethanol production in batch fermentation with different yeast strains was tested. The results are summarized in figure 1 middle.

[0385] method:

[0386] Pass ten different media through RED STAR TM Batch fermentation with yeast and RWB218 (Nedalco) to produce ethanol. Media 1 to 5 were glucose and xylose solutions supplemented with 0.5% (w / v) yeast extract and 1% (w / v) peptone. Media 6 to 10 were filtered unwashed pretreated corn stover (fuwPCS) enzymatic hydrolyzates with varying levels of total solids. The sugar levels of media 5 to 9 were adjusted so that the xylose and glucose concentrations were equivalent to those found in the fuwPCS hydrolyzate at 20% total solids to test the resistance of the strains to different levels of inhibition. All media were filter sterilized.

[0387] Medium 1: onl...

Embodiment 3

[0400] Ethanol production for high cell counts

[0401] High RED STAR was tested by inoculating filtered pretreated corn stover (PCS) enzymatic hydrolyzate at various starting yeast cell concentrations TM Effect of yeast feeding (cell count) on ethanol production in batch fermentation. The results are summarized in figure 2 middle.

[0402] method:

[0403] Unwashed PCS (acid catalyzed from The National Renewable Energy Laboratory, Golden, CO) was digested at 50°C, pH 5.0 using an initial solids concentration of 20% (w / w) and 50 mg Cellulase Preparation A / g cellulose. , pretreated corn stover) hydrolyzed for 120 hours. After hydrolysis, the slurry was centrifuged at 3000 rpm for 10 minutes using a Beckman-Coulter countertop centrifuge to separate the solids. The resulting liquid hydrolyzate was supplemented with nutrients: yeast extract and peptone at a level of 5 g / L each, and passed through a 250 mL nalgene bottle with a working volume of 150 mL using a shaking inc...

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Abstract

The invention relates to methods for producing a fermentation product from a lignocellulose-containing material comprising i) pre-treating lignocellulose-containing material; ii) hydrolysing pre-treated lignocellulose-containing material; iii) fermenting using a fermenting organism; wherein fermentation is initiated and carried out at: a) a fermentation organism cell count in the range from 10-250x1010 cells per L fermentation medium; or b) a fermentation organism concentration in the range from 2-90 g dry weight fermenting organism per L fermentation medium.

Description

technical field [0001] The present invention relates to methods of producing fermentation products from lignocellulose-containing material using one or more fermenting organisms. Background technique [0002] Due to the limited reserves of fossil fuels and concerns about greenhouse gas emissions, there is increasing interest in the use of renewable energy sources. [0003] Production of fermentation products from lignocellulose-containing material is known in the art and includes pretreatment, hydrolysis and fermentation of lignocellulose-containing material. [0004] The fermentation step is performed using a fermenting organism capable of converting fermentable sugars into the desired fermentation product. After a fermenting organism is inoculated into a fermentation medium, it goes through a number of stages. The initial phase is called the "lag phase" and is an adaptation phase in which no significant amount of fermentation product is produced. Significant amounts of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
CPCC12P7/10Y02E50/17Y02E50/16C12P19/14Y02E50/10C12P1/00
Inventor 马兹·T·史密斯吉勒莫·科沃德-凯利丹·尼尔森康正芳普拉尚特·艾耶兰迪·戴因哈默
Owner NOVOZYMES AS
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