Method for detecting microcystin-LR by self-assembly based on end face of gold nano-rod

A microcystin and gold nanorod technology, applied in the field of immunoassay chemistry, can solve the problems of lack of commercial supply of specific protein phosphatase, inability to meet the requirements of rapidity, convenience and accuracy, poor specificity, etc., and achieve a simplified reaction. conditions, reduce labor intensity, and improve the effect of sensitivity
CN101699288AInactive Publication Date: 2010-04-28JIANGNAN UNIV
0 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2010-04-28
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for detecting microcystin-LR by self-assembly based on the end face of a gold nano-rod, belonging to the technical field of chemical immunoassay. In the invention, the end face of the gold nano-rod is respectively coupled with microcystin coating antigen and anti-microcystin antibody to form a probe of the gold nano-rod; and the synthesized probe of the gold nano-rod performs immunological reaction with the microcystin-LR so as to achieve the detection of the microcystin-LR through the change of the grain diameter of a nano-chain which is formed by the antigen antibody reaction of the end face of the gold nano-rod. The invention performs reaction in a liquid environment, only one step of reaction is needed without washing step, which simplifies the condition of the reaction, reduces labour intensity and improves the detection sensitivity.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention discloses a method for detecting microcystin-LR (MC-LR) based on the end face self-assembly of gold nanorods, which belongs to the technical field of immunoanalysis chemistry. Background technique

[0002] With the development of the economy, sewage containing a large amount of nitrogen and phosphorus nutrients enters lakes and reservoirs, causing eutrophication of water bodies, resulting in abnormal proliferation of algae, releasing secondary metabolites algal toxins (Algae Toxins), threatening the safety of human drinking water and the safety of other organisms in the water. Among them, Microcystins (MCs) are the most widely distributed and most harmful, and their structures are as follows:

[0003]

[0004] It contains five fixed amino acids and two variable amino acids X, Y. That is, in the above structural formula, X and Y are Leu and Arg respectively, which is MC-LR. MC-LR is the most common and most toxic microcystin. The max...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More