Method for inducing gonad maturity and ovulation of verasper variegates parent fish by implanting slow-release hormone

Abstract

The invention relates to a method for inducing gonad maturity and ovulation of a verasper variegates parent fish by implanting a slow-release hormone, which comprises two aspects of cultivating an optimally selected parent fish and adjusting temperature and light to promote the gonad development of the parent fish, and implanting the slow-release hormone for promoting a metrocyte of the parent fish to reach final maturity and ovulation. In the invention, the slow-release hormone is a salmon gonadotropin-releasing hormone analog (sGnRHa) and is coated with a safe and high-efficiency polymer slow-release transitive system (95 percent of cholesterol and 5 percent of cellulose); a female parent fish with gonad in fourth stage is selected by using a living body sampling method or a light tablemethod; a special implanting gun is used for implanting in back muscles, and the effect time of the slow-release hormone lasts for 14-18 days. The method can enable the metrocyte of the verasper variegates parent fish to achieve final maturity and ovulation, has low duress on parent fish reproduction, high rate of parent fish survival and gonad maturity, high fertility rate being 72 percent and high hatching rate being 56.2 percent, and can acquire a bulk of high-quality oosperms. The invention is a set of complete control technique method, can stably regulate and control the development, thematurity and the ovulation of the gonad of the verasper variegates parent fish, and acquire high-quality oosperms.

Description

Technical field: [0001] The invention belongs to the breeding technology of star flounder broodstock, and is a method for artificially implanting slow-release hormones to promote the final maturation and ovulation of oocytes of the star flounder broodstock, and to obtain batches of high-quality fertilized eggs. technical background: [0002] The round-spotted star flounder (Verasper variegates T.&S.) is mainly distributed in the Yellow Sea, Bohai Sea, East my country Sea, Japan, North Korea and other waters of China, and its output is not much. In the bay, there is no long-distance migration, and it is an excellent variety for resource proliferation and artificial breeding. Star flounder has a beautiful appearance, large body, tender and delicious meat, thick and elastic fin edges, rich in various vitamins and trace elements, rich in nutrition, easy to cook, popular with consumers, and has great market development potential. At present, the natural resources of star flounder...

AI Technical Summary

Problems solved by technology

Effect of LH-RHa cholesterol pellet on gonadalmaturation in spotted halibut.) successfully induced broodstock spawning, but the spawning efficiency of broodstock was not high, and the egg quality of each batch was quite different, so the seed production has not been able to achieve a big breakthrough

In recent years, researchers in my country have used HCG and LHRH combined with intramuscular injection in the back to induce the spawning of star flounder (Dalian Tianzheng Industrial Co., Ltd., Meng Xuesong et al., 2007), but the mortality rate of broodstock reached 20-30%, and successful ovulation The ratio of broodstock is low, the egg quality of each batch varies greatly, and the fertilization rate is low

Zhang Quanqi et al. (Ocean University of China, 2004) established a method of slow-release hormones to induce oviposition in star flounder, mainly by intraperitoneal injection of liquid slow-release hormones, which can promote the development and maturation of parent fish gonads, but did not mention the maturation of brood fish eggs, Parental survival and specific oviposition effects, and the risk factor of intraperitoneal injection is high

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