Tumor cell drug resistance detecting chip

Abstract

The invention discloses a tumor cell drug resistance detecting chip, which comprises a chip body. The surface of the chip body is provided with a medicament inlet, a culture solution inlet and a cell inlet; a medicament passage and a culture solution passage are arranged in the chip body; a radial aggregation passage is arranged at a position where the medicament passage and the culture solution passage meet; the radial end of the aggregation passage is communicated with a plurality of pipelines; each pipeline is separated into small pipes; a cell pool is arranged on each small pipe; the other end of each pipeline and the other end of each small pipe are connected with a cell inlet passage which is communicated with the cell inlet; the lower part of the cell pool is provided with a pH indicating layer; a carbon dioxide permeable membrane is arranged between the cell pool and the pH indicating layer; and an acid-base indicator is placed in the pH indicating layer. The tumor cell drug resistance detecting chip has a simple structure and can simulate blood capillaries in vivo and complete multi-parameter analysis related to a multiple drug resistance mechanism.

Description

technical field [0001] The invention relates to a drug resistance detection chip, in particular to a drug resistance detection chip for tumor cells. Background technique [0002] In recent years, the incidence and mortality of cancer in my country have been on the rise. Chemotherapy is currently the main means of treating malignant tumors. The long-term use of traditional chemotherapy drugs has made tumor cell drug resistance more and more common, and has become the most common and most difficult problem that leads to the failure of clinical tumor chemotherapy. Among them, multi-drug resistance is the main form of tumor cell drug resistance, which means that after a drug acts on tumor cells to produce drug resistance, the tumor cells gradually become resistant to untouched, structurally irrelevant, target and mechanism of action. Different multiple antineoplastic drugs also have cross-resistance. Therefore, the detection of drug resistance of tumor cells is of great signif...

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Problems solved by technology

[0003] (1), unable to dynamically detect the interaction process between tumor cells and drugs, such as changes in the pH of the tumor extracellular microenvironment;

[0004] (2), the experimental drug can not automatically form a continuous drug concentration gradient, it is difficult to optimize the effective drug concentration;

[0006] (4), it is not possible to study multiple drug resistance mechanisms at the same time, such as the proton pump function and efflux pump function of tumor cells (P glycoprotein pump function, P -gp), detection of intracellular reduced glutathione, etc.

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