Production method for preparing inulase

A production method and a technology for inulinase, which are applied in the field of preparing inulinase, can solve the problems of high application cost, low yield of inulinase of enzyme-producing strains, restricting the application of fructooligosaccharides and high fructose syrup, and the like. Simple, enhanced permeability, stable and reliable production process

Inactive Publication Date: 2012-05-23
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The inulinase yield of enzyme-producing strains is low, resulting in high application costs, which restricts the application of fructooligosaccharides and high fructose syrup in the food industry and feed industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: A production method for preparing inulinase

[0031] Preparation of spore suspension: Aspergillus niger was inoculated on the slant medium. The composition of the slant medium was (g / L): peptone 10, inulin 40 and agar 20. The pH value of the slant medium was natural value. Place the inoculated slant culture medium in a thermostat at 28°C for 3 days to produce spores. After removing the spores, add sterile normal saline with a concentration of 0.85% to wash the spores to prepare a spore suspension. Then dilute the spore suspension with 0.85% sterile normal saline to 10 spores per ml of liquid 7 Spare.

[0032] The number of spores per ml of liquid is 10 7 Each detection method can be measured by the plate colony counting method.

[0033] Preparation of liquid seeds: The composition of the liquid seed culture medium is (g / L): yeast powder 15, peptone 15 and inulin 40, and the pH value of the liquid seed culture medium is a natural value. Heat the liquid seed culture...

Embodiment 2

[0036] Example 2: A production method for preparing inulinase

[0037] Preparation of spore suspension: Aspergillus niger was inoculated on the slant medium. The composition of the slant medium was (g / L): peptone 10, inulin 40 and agar 20. The pH value of the slant medium was natural value. Place the inoculated slant culture medium in a thermostat at 28°C for 3 days to produce spores. After removing the spores, add sterile normal saline with a concentration of 0.85% to wash the spores to prepare a spore suspension. Then dilute the spore suspension with 0.85% sterile normal saline to 10 spores per ml of liquid 7 Spare.

[0038] The number of spores per ml of liquid is 10 7 Each detection method can be measured by the plate colony counting method.

[0039] Preparation of liquid seeds: The composition of the liquid seed medium is (g / L): yeast powder 17, peptone 17 and inulin 35, and the pH value of the liquid seed medium is a natural value. Heat the liquid seed culture medium to 121°C...

Embodiment 3

[0042] Example 3: A production method for preparing inulinase

[0043] Preparation of spore suspension: Aspergillus niger was inoculated on the slant medium. The composition of the slant medium was (g / L): peptone 10, inulin 40 and agar 20. The pH value of the slant medium was natural value. Place the inoculated slant medium in a thermostat at 28°C for 4 days to form spores. After removing the spores, add sterile normal saline with a concentration of 0.85% to wash the spores to prepare a spore suspension. Then dilute the spore suspension with 0.85% sterile normal saline to 10 spores per ml of liquid 7 Spare.

[0044] The number of spores per ml of liquid is 10 7 Each detection method can be measured by the plate colony counting method.

[0045] Preparation of liquid seeds: The composition of the liquid seed medium is (g / L): yeast powder 10, peptone 10 and inulin 50, and the pH value of the liquid seed medium is a natural value. Heat the liquid seed culture medium to 121°C and steril...

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PUM

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Abstract

The invention relates to a production method for preparing inulase. The method comprises the following four steps of: the preparation of a spore suspension, the preparation of liquid seeds, the preparation of an aspergillus niger fermentation liquor and the preparation of an inulase product. The method specifically comprises the following steps of: inoculating aspergillus niger stains on a slant culture medium to culture the spore suspension; inoculating the spore suspension into a liquid seed culture medium at a temperature of 28-30 DEG C to culture so as to obtain the liquid seeds; inoculating the liquid seeds into an aspergillus niger fermentation medium to ferment according to an inoculation quantity of 3-7 percent in a volume ratio under an aseptic condition; within a period of time of 0-60h under a certain fermentation condition, opening an ultrasonic transmitting device to carry out ultrasonic irradiation culture on the aspergillus niger fermentation medium and the liquid seeds; after the ultrasonic irradiation culture, continuously carrying out ultrasonic irradiation culture to obtain the aspergillus niger fermentation liquor; precipitating inulase from the aspergillus niger fermentation liquor by adopting a salting-out method; and filtering waste liquid through a filter press and drying a filter cake to obtain an inulase product. The production method is simple and stable, saves the production cost, and improves the output of the inulase.

Description

Technical field [0001] The invention belongs to the technical field of biological fermentation, and particularly relates to a production method for preparing inulinase. Background technique [0002] Inulinase, scientific name (β-2,1-D-fructanase), also called (β-fructanase), or [2,1-D-fructanase (EC 3.2.1 )]. It is a polysaccharide hydrolase that can hydrolyze the fructoside bond of (β-2,1-D-fructan). [0003] The current application prospects of inulinase are mainly reflected in three aspects: [0004] 1) Use inulinase to hydrolyze inulin to produce high fructose syrup. Because high fructose syrup is cheap, sweet, refreshing, high osmotic pressure, good preservation effect, low calorific value, not easy to cause dental caries, and can be used by diabetic patients, it is widely used in the food and pharmaceutical industries in developed countries such as the United States and Japan. . Compared with the traditional method of producing fructose by using starch as a raw material an...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N9/24C12R1/685
Inventor罗登林钟先锋李佩艳辛莉李爱江袁海丽徐宝成刘建学许威王佳莹
OwnerHENAN UNIV OF SCI & TECH