Egg hatching method for culturing transgenic poultry

Abstract

The invention discloses an egg hatching method for culturing transgenic poultry. The method comprises the following steps: placing hatching eggs with closed open holes on equators of eggshells in a way of keeping the closed open holes upward and at the top, standing and hatching; further placing the eggs by keeping big heads upward and keeping small heads downward when chorions in the eggs develop to wrap all contents in the eggs, carrying out egg turning and hatching; and then standing and hatching till nestling. The method can overcome the defect of spillover of egg-white of the traditional method, ensure the balance of water and nutrition in the eggs during the hatching process, reduce the loss of death of chicken embryos due to the spillover of the egg-white, ensure the normal development of the transgenic chicken embryos and improve the hatching rate of the transgenic chicken embryos. The method has simple steps and strong operability, and is applicable to hatching the poultry hatching eggs after transgenic operation by adopting other window-opening methods, such as quails, domestic ducks and the like. Therefore, the method has important application value and is applicable to popularization.

Description

technical field [0001] The invention relates to a breeding egg hatching method for cultivating transgenic poultry. Background technique [0002] Unfavorable genes in poultry can be eliminated by using genetic engineering technology to improve disease resistance and stress resistance of poultry, improve poultry production performance and improve meat quality. Following the mammary gland bioreactor, transgenic technology is used to introduce human or animal antibody genes into the laying hen and transform it into an oviduct bioreactor, so that the eggs produced can contain a large amount of protein with economic value, and then use its Manufacture of pharmaceuticals and healthcare products. Therefore, transgenic poultry has great economic value and application prospect. [0003] In recent years, many research reports on transgenic chickens have been released, but few of them can be put into production. One of the main reasons is that it is difficult to successfully hatch tra...

AI Technical Summary

Problems solved by technology

However, compared with the actual demand, the hatching rate of this method is still very low, mainly because egg white spills generally occur in the sealed eggs during the hatching process, and at most 100% of a batch of eggs may have different degrees of egg white spills Phenomenon, the overflow of egg white destroys the balance of moisture and nutrition in the egg, affects the normal development of chicken embryos, and the overflowing egg white is very easy to breed bacteria, causing bacterial pollution, resulting in the death of chicken embryos

Relevant studies believe that the egg white overflow is caused by the imbalance of the air pressure inside the egg caused by opening the window. Therefore, the solution is to seal the window and add dilute egg white or phosphate buffer solution to the egg, and also remove the eggshell at the blunt end of the egg. Although such measures have partially reduced the degree of egg white spillage, these methods are cumbersome to operate and have not fundamentally prevented the occurrence of egg white spillage

Images