Method for culturing organophosphorus pesticide degrading bacteria
A technology for organophosphorus pesticides and degrading bacteria, which is applied in the biological field to achieve the effects of low production cost and resource utilization
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Embodiment 1
[0020] Embodiment 1: organophosphorus pesticide degrading bacterial agent cultivation method
[0021] 1. Medium preparation:
[0022] 1) Strain preservation medium (solid): 2g ammonium sulfate, 2g dipotassium hydrogen phosphate, 0.1g magnesium sulfate, 20g agar, add sweet potato starch wastewater to 1L, pH 7.0-7.5;
[0023] 2) Strain activation medium (liquid): 2g ammonium sulfate, 2g dipotassium hydrogen phosphate, 0.1g magnesium sulfate, add sweet potato starch wastewater to 1L, pH 7.0-7.5;
[0024] 3) Seed medium (liquid): 20g ammonium sulfate, 20g dipotassium hydrogen phosphate, 1.0g magnesium sulfate, add sweet potato starch wastewater to 10L, pH 7.0-7.5;
[0025] 4) Fermentation medium (liquid): 0.5kg ammonium sulfate, 20g magnesium sulfate, 0.4kg dipotassium hydrogen phosphate, add sweet potato starch wastewater to 200L, pH 7.0-7.5;
[0026] All the above media were sterilized at 121°C for 15-30min.
[0027] 2. Strain transfer and activation: Pick the organophosphoru...
Embodiment 2
[0031] Example 2: Degradation effect of organophosphorus pesticide degrading bacteria agent on dichlorvos pesticide residues on rape leaves
[0032] 1) Select two identical rape plots, each 30m 2 , evenly spray 1000 times diluted 80% dichlorvos EC pesticide;
[0033] 2) After 12 hours of spraying dichlorvos, the pests have been killed, and the pesticide degrading bacteria agent (diluted 600 times with water) is evenly sprayed, and the spraying of clean water is used as a control;
[0034] 3) After 24 hours of spraying the fungicide, the five-point sampling method was used to detect the residue of dichlorvos on the leaves of rapeseed. It was found that the treatment group sprayed with the fungicide compared with the control group without spraying the fungicide, the residual amount of dichlorvos was significantly reduced, which was lower than that of the countries with residual dichlorvos in vegetables. Limit standard (0.1mg / kg).
Embodiment 3
[0035] Embodiment 3: Degradation effect of organophosphorus pesticide degrading bacteria agent on rape leaf dimethoate
[0036] 1) Select two identical rape plots, each 30m 2 , evenly spray 1000 times diluted 50% Dimethoate EC pesticide;
[0037] 2) After 12 hours, evenly spray the pesticide degrading bacteria agent (diluted 500 times with water), and use spraying water as a control;
[0038] 3) After 48 days, the five-point sampling method was used to detect dimethoate residues on rapeseed leaves, and it was found that the residues of dimethoate in the treatment group were significantly lower than those in the control group, which was lower than the national limit standard for dimethoate residues in vegetables.
[0039] The bacterial strain that degrades organophosphorus pesticides provided by the present invention is preserved in the General Microorganism Center of China Microbial Strain Preservation Management Committee. The preservation date is November 2, 2009. The class...
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