Marine bacteria chromogenic isolation medium and application thereof

A technology of marine bacteria and culture medium, applied in the direction of bacteria, microorganism-based methods, microorganism measurement/inspection, etc., can solve the problems of missing microorganisms, underestimating microbial diversity, unfavorable marine bacteria isolation and diversity research, etc. Achieve the effects of increasing species, broad application value and prospects, and benefiting the research of bacterial diversity and microbial resources
CN101735968AInactive Publication Date: 2010-06-16SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2010-06-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a marine bacteria chromogenic isolation medium and application thereof. The formula of the marine bacteria chromogenic isolation medium comprises the following compositions: 2 to 3g / L of peptone, 0.1 to 1g / L of yeast cream, 8 to 20 g / L of lactose, 8 to 20 g / L of sucrose, 8 to 20 g / L of cellobiose, 0.1 to 1 g / L of sodium thiosulfate, 0.1 to 1 g / L of ammonium ferric citrate, 0.01 to 0.1 g / L of 3-amino-7-dimethylamino-2-methylphenazine hydrochloride and 10 to 20 g / L of agaragar. The marine bacteria chromogenic isolation medium can make bacteria growing positions have different colors, is convenient to detect or separate marine bacteria quickly and simply, and facilitates marine microorganism diversity study.
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Description

technical field

[0001] The invention relates to the field of separation and cultivation of marine bacteria, in particular to a chromogenic separation medium for marine bacteria and its application. Background technique

[0002] For a long time, the culture medium mainly used for the separation of marine bacteria are Zobell 2216E and TSB (Tryptone Soy Broth), etc. Among them, Zobell 2216E is the most widely used, consisting of peptone 5.0g / L, yeast extract 1.0g / L, phosphoric acid Iron 0.01g / L, agar 18.0g / L and seawater 1000mL. Because peptone and yeast extract in this medium only provide basic carbon and nitrogen sources, vitamins, and growth factors, most of the bacterial colonies grown on the plate are only white, and occasionally a very small amount of yellow colonies appear. Using this type of medium, it is almost impossible to distinguish different species of bacteria in terms of culture morphology. In addition, in the actual microbiological work, it was found that usi...

Claims

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