Marine bacteria chromogenic isolation medium and application thereof

A technology of marine bacteria and culture medium, applied in the direction of bacteria, microorganism-based methods, microorganism measurement/inspection, etc., can solve the problems of missing microorganisms, underestimating microbial diversity, unfavorable marine bacteria isolation and diversity research, etc. Achieve the effects of increasing species, broad application value and prospects, and benefiting the research of bacterial diversity and microbial resources

Inactive Publication Date: 2010-06-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The use of such conventional media is not conducive to the isolation and diversity of marine bacteria, often resulting in some valuable microorganisms being missed or underestimating the diversity of microorganisms

Method used

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  • Marine bacteria chromogenic isolation medium and application thereof
  • Marine bacteria chromogenic isolation medium and application thereof
  • Marine bacteria chromogenic isolation medium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Bacteria are cultivated on the substratum of the present invention in the seawater of embodiment 1

[0018] Medium of the present invention: peptone 2.5g, yeast extract 0.5g, iron phosphate 0.05g, lactose 10g, sucrose 10g, cellobiose 10g, sodium thiosulfate 0.5g, ferric amine citrate 0.5g, neutral red 0.025g, Agar 18g, seawater 1000mL, after stirring evenly, sterilize at 121°C for 20 minutes, adjust the pH to 7.4~7.6 when cooled to 65~60°C, pour it into a plate, solidify and dry before use.

[0019] Zobell 2216E medium: peptone 5.0g / L, yeast extract 1.0g / L, iron phosphate 0.01g / L, agar 18.0g / L, seawater 1000ml, stir well, sterilize at 121°C for 20 minutes, cool to 65~60°C Pour over the plate, set aside after drying (as a control).

[0020] Take fresh coastal seawater, absorb 100-500 μL (adjusted according to the bacteria content in the water), apply it on the culture medium of the present invention and Zobell 2216E medium respectively, and incubate in a 30°C incubator ...

Embodiment 2

[0022] Bacteria in the water of embodiment 2 seawater prawn cultivation ponds are cultivated on the culture medium of the present invention

[0023] Medium of the present invention: peptone 2.5g, yeast extract 0.5g, iron phosphate 0.05g, lactose 10g, sucrose 10g, cellobiose 10g, sodium thiosulfate 0.5g, ferric amine citrate 0.5g, neutral red 0.025g, Agar 18g, seawater 1000ml, after stirring evenly, sterilize at 121°C for 20 minutes, adjust the pH to 7.4~7.6 when cooled to 65~60°C, pour it into a plate, solidify and dry before use.

[0024] Zobell 2216E medium: peptone 5.0g / L, yeast extract 1.0g / L, iron phosphate 0.01g / L, agar 18.0g / L, seawater 1000ml, stir well, sterilize at 121°C for 20 minutes, cool to 65~60°C Pour over the plate, set aside after drying (as a control).

[0025] Take fresh mariculture pond water, draw 100-500 μ L (adjusted according to the bacteria content in the water), apply it on the medium of the present invention and Zobell 2216E medium respectively, an...

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Abstract

The invention discloses a marine bacteria chromogenic isolation medium and application thereof. The formula of the marine bacteria chromogenic isolation medium comprises the following compositions: 2 to 3g/L of peptone, 0.1 to 1g/L of yeast cream, 8 to 20 g/L of lactose, 8 to 20 g/L of sucrose, 8 to 20 g/L of cellobiose, 0.1 to 1 g/L of sodium thiosulfate, 0.1 to 1 g/L of ammonium ferric citrate, 0.01 to 0.1 g/L of 3-amino-7-dimethylamino-2-methylphenazine hydrochloride and 10 to 20 g/L of agaragar. The marine bacteria chromogenic isolation medium can make bacteria growing positions have different colors, is convenient to detect or separate marine bacteria quickly and simply, and facilitates marine microorganism diversity study.

Description

technical field [0001] The invention relates to the field of separation and cultivation of marine bacteria, in particular to a chromogenic separation medium for marine bacteria and its application. Background technique [0002] For a long time, the culture medium mainly used for the separation of marine bacteria are Zobell 2216E and TSB (Tryptone Soy Broth), etc. Among them, Zobell 2216E is the most widely used, consisting of peptone 5.0g / L, yeast extract 1.0g / L, phosphoric acid Iron 0.01g / L, agar 18.0g / L and seawater 1000mL. Because peptone and yeast extract in this medium only provide basic carbon and nitrogen sources, vitamins, and growth factors, most of the bacterial colonies grown on the plate are only white, and occasionally a very small amount of yellow colonies appear. Using this type of medium, it is almost impossible to distinguish different species of bacteria in terms of culture morphology. In addition, in the actual microbiological work, it was found that usi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/01
Inventor 苏婷罗鹏胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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