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Method for testing microcystin (MC) toxin in M1rA gene cDNA sequence, deduced amino acid and water body
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A technology of microcystin and detection method, applied in the field of environmental detection, can solve the problems of large workload, high cost, inability to completely remove MC and the like
Inactive Publication Date: 2010-07-14
JINAN UNIVERSITY
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These degradation technologies have many problems such as high cost, heavy workload, and inability to completely remove MC.
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Embodiment 1
[0045] Example 1 Obtaining of cDNA sequence of MlrA gene and its encoded amino acid sequence
[0047] The water samples used in the experiment were collected from Xiangang Reservoir, Huizhou City, Guangdong Province, Minghu Lake, Jinan University, Guangzhou City, and Guangdong Tilapia Breeding Farm, and fish gut samples were collected from Xiangang Reservoir, Huizhou City, Guangdong Province.
[0048] Water samples and fish intestinal samples were collected in March, April, May, July, August, October, November and December, covering four seasons of the year.
[0049] Water samples were collected from surface water bodies at various locations with sampling bottles.
[0050] The fish intestines were killed on the spot, and the hindguts (about 10 cm) were taken, and the excreta in the intestines were picked out and mixed with 15 ml of distilled water to obtain fish intestine samples.
[0051] After mixing the above water sample and f...
Embodiment 2
[0069] Example 2 MlrA detection results in water bodies and fish bodies in different seasons
[0071] The water samples used in the experiment were collected from Xiangang Reservoir, Huizhou City, Guangdong Province, Minghu Lake, Jinan University, Guangzhou City, and Guangdong Tilapia Breeding Farm, and fish gut samples were collected from Xiangang Reservoir, Huizhou City, Guangdong Province.
[0072] In March, April, May, July, August, October, November and December, water samples and fish intestinal samples were collected respectively.
[0073] Water samples were collected from surface water bodies at various locations with sampling bottles.
[0074] The fish gut samples were killed on the spot, and the hindguts (about 10 cm) were taken, and the excreta in the guts were picked out and mixed with 15 ml of distilled water to obtain the fish gut samples.
[0075] After the water samples and fish intestinal samples in the water envi...
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Abstract
The invention provides a method for testing microcystin (MC) toxin in an M1rA gene cDNA sequence, a deduced amino acid and a water body. In the M1rA gene cDNA sequence, the nucleotide sequence is as shown in SEQ ID No: 1, and the deduced amino acid sequence is as shown in SEQ ID NO: 3. By the research of water environment samples in different seasons under different environments, the invention finds that the existence of the M1rA gene cDNA sequences in the surface water body microorganism and the fish intestinal canal has the certain relationship with the content of the MC toxin in the water body, thus a segment of new M1rA gene cDNA sequence and the deduced amino acid sequence thereof are finally obtained by selecting and searching primer designs, polymerasechain reaction (PCR) amplification reaction conditions and reaction systems, and the MC toxin in the water body is tested by combining the characteristics of uniqueness and specificity of the M1rA gene cDNA sequence on the MC toxin and utilizing the simple PCR amplification technology.
Description
technical field [0001] The invention relates to the technical field of environmental detection, in particular to the detection of microcystins in the environment by biological methods, in particular to the cDNA sequence of the MlrA gene, the coded amino acid and the detection method of the microcystins in water. Background technique [0002] Microcystin (MC) is a type of algal toxin that occurs most frequently in cyanobacterial blooms and causes the most serious harm. It can cause poisoning and death of wild animals, livestock, poultry, etc., and cause human liver damage and high incidence of liver cancer. . More than 60 isomers of this toxin have been identified, the most common of which are MC-LR, MC-RR, and MC-YR. [0003] Regarding the biodegradation of MC, Bourne et al. believed that the degradation of MC-LR was at least caused by three hydrolytic enzymes in the cell. Bourne et al. (Bourne D G, Riddles P, Jones G J, et al.2001.Characterization of a gene cluster involv...
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