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Method for extracting liensinine, isoliensinine and neferine from lotus plumule

A technology of liensinine and isoliensinine, which is applied in the field of isoliensinine, liensinine, and the extraction and separation of high-purity liensinine, can solve the problems of not meeting market demand, small production scale, high production cost, etc., and achieve shortening Separation time, saving reagents, and short process cycle

Inactive Publication Date: 2010-07-28
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned various methods may obtain products with lower purity, or lower yields, or use more complicated systems, and some have higher production costs and smaller production scales, which cannot meet market demand.

Method used

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  • Method for extracting liensinine, isoliensinine and neferine from lotus plumule
  • Method for extracting liensinine, isoliensinine and neferine from lotus plumule
  • Method for extracting liensinine, isoliensinine and neferine from lotus plumule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 100g of crushed lotus seed heart medicinal material, add 0.3L 50% ethanol, heat and reflux for 60 minutes, separate the residue and extract; add 0.3L 50% ethanol to the residue and reflux twice, each time for 40 minutes, separate the residue and extract; combine the extracts obtained by reflux for the above 3 times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of lotus seed heart.

[0027]Suspend the above crude extract in water, put it on a D101 macroporous adsorption resin column, elute with 10% ethanol of 10 times the column volume, and discard the eluent. Then elute with 40% ethanol until there is no liensinine, isoliensinine and methyl-liensinine in the effluent, collect the eluate, and distill under reduced pressure to recover the solvent to obtain the extract of lotus root.

[0028] Diethyl ether-phosphate buffer was used as a high-speed countercurrent chromatography two-phase solvent system to separate and purify th...

Embodiment 2

[0030] Take 100g of crushed lotus seed heart, add 2L of 55% ethanol, heat and reflux for 20 minutes, separate the residue and extract; add 1L of 55% ethanol to the residue and reflux twice, each time for 10 minutes, separate the residue and extract liquid; combine the extracts obtained by reflux for the above 3 times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of lotus seed heart.

[0031] Suspend the above-mentioned crude extract extract in water, put it on HPD300 macroporous adsorption resin column, elute with 30% ethanol of 5 times the column volume, and discard the eluent. Then elute with 80% ethanol until there is no liensinine, isoliensinine and methyl-liensinine in the effluent, collect the eluate, and distill under reduced pressure to recover the solvent to obtain the extract of lotus root.

[0032] Diethyl ether-phosphate buffer was used as a high-speed countercurrent chromatography two-phase solvent system to separate and p...

Embodiment 3

[0034] Take 100g of crushed lotus seed heart, add 0.5L 60% ethanol, heat and reflux for 45 minutes, separate the residue and extract; add 0.5L 60% ethanol to the residue and reflux twice, each time for 30 minutes, separate the residue and extract; combine the extracts obtained by reflux for the above 3 times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of lotus seed heart.

[0035] Suspend the above-mentioned crude extract extract in water, put it on a D101 macroporous adsorption resin column, and elute with 15% ethanol of 10 times the column volume, and discard the eluent. Then elute with 60% ethanol until there is no liensinine, isoliensinine and methyl-liensinine in the effluent, collect the eluate, and distill under reduced pressure to recover the solvent to obtain the extract of lotus root.

[0036] Diethyl ether-phosphate buffer was used as a high-speed countercurrent chromatography two-phase solvent system to separate and purif...

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Abstract

The invention relates to a method for extracting liensinine, isoliensinine and neferine from lotus plumule, which adopts the lotus plumule as a raw material and comprises the following steps of: (1) preparing a crude extract; (2) preseparating by adopting macroporous resin; (3) purifying by adopting a high speed counter current chromatography to obtain the liensinine, the isoliensinine and the neferine with the purity of more than 98 percent. By extracting alkaloid in a lotus plumule medicinal material by adopting ethanol, carrying out preseparation by adopting macroporous resin, adopting the ethanol as an eluant and carrying out purification by adopting the high speed counter current chromatography, the process is environmental-friendly without serious damage to the environment.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for extracting and separating high-purity liensinine, isoliensinine and methyl-liensinine from the heart of lotus seeds, a Chinese herbal medicine. Background technique [0002] Lotus seed heart (Nelumbo nucifera) is the green young leaves and radicles of lotus seeds of Nymphaeaceae plants. It is bitter and cold in nature and has the effect of clearing heart and removing heat. The main active ingredient is alkaloids. Liensinine has a good antiarrhythmic effect, which can significantly resist the arrhythmia induced by aconitine in rats and guinea pigs induced by ouabain; it can also prevent the occurrence of ventricular fibrillation in guinea pigs caused by adrenaline; it can also resist myocardial infarction. Cardiac arrhythmia in rats induced by blood reperfusion. Isoliensinine intravenous injection can dose-dependently and transiently reduce the heart rate...

Claims

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Application Information

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IPC IPC(8): C07D217/20
CPCY02P20/582
Inventor 孙爱玲柳仁民
Owner LIAOCHENG UNIV
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