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Purification method of human chorionic gonadotropin (HCG)

A technology of chorionic gonadotropin and purification method, applied in the field of purification of gonadotropin

Active Publication Date: 2012-05-30
LIVZON PHARM GRP INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, one-step cation exchange resin is often used for purification to obtain high-quality products with a unit potency greater than 2500iu / mg. The yield of these methods is generally less than 80%, and the potency of high-quality products is around 3000iu / mg.

Method used

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  • Purification method of human chorionic gonadotropin (HCG)
  • Purification method of human chorionic gonadotropin (HCG)

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Experimental program
Comparison scheme
Effect test

Embodiment

[0018] Process operation process:

[0019] The purification process flow chart that embodiment uses is as figure 1 As shown, the specific operation method is as follows:

[0020] 1 Mixing, extraction, adsorption, elution:

[0021] 1.1 Mixing and extraction:

[0022] Accurately weigh the crude chorionic gonadotropin and add it to a stainless steel barrel, then add 20 times the volume of solution A and stir for extraction. The stirring speed is 200-400 rpm, the temperature is controlled at 0-15°C, and the extraction is 1-1.5 hours. Centrifuge for 30 minutes at 4000 rpm at 2-6°C inside the machine, take the supernatant, and extract the slag with 10 times the volume of solution A for 1 hour, then centrifuge at 4000 rpm at 2-6°C inside the machine. Centrifuge at 6°C for 30 minutes, and take the supernatant. Combine the two supernatants, and adjust the pH value to 4.5-5.0 with 5 mol / L sodium hydroxide.

[0023] 1.2 Adsorption and elution:

[0024] Place the above solution at a...

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Abstract

The invention provides a purification method of human chorionic gonadotropin (HCG) extracted from the urine of pregnant women. Compared with the existing extraction and purification method of the HCG in the prior art, the method of the invention has the advantage of higher yield, and the unit valence can reach more than 5000 iu / mg.

Description

technical field [0001] The invention relates to a method for purifying gonadotropin, in particular to a method for purifying chorionic gonadotropin extracted from urine of pregnant women. Background technique [0002] Gonadotropin (gonadotropin, gonadotropic hormone) refers to a class of hormones that can promote the development of both male and female gonads and increase the secretion of sex hormones, such as luteinizing hormone (LH) and follicle stimulating hormone (FSH) secreted by the anterior pituitary gland. Chorionic gonadotropin (HCG) secreted by the placental chorion. Among them, HCG can stimulate the gonad vitality of women or men, promote the development of gonads in the body, and maintain or stimulate the sexual function. After taking HCG in an appropriate amount, the sexual secretion function that has declined or has pathological disorders can gradually return to normal. HCG is synthesized and secreted by the syncytiotrophoblasts of the human placental chorion ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/30C07K1/18C07K14/59
Inventor 万龙岩陈辉阮振兴
Owner LIVZON PHARM GRP INC