Gene vaccine of sperm-specific cationic channel protein CatSper1 and preparation method thereof
A technology of gene vaccine and channel protein, which is applied in the field of preparation of the vaccine, can solve the problems of reduction, loss of fertilization ability, loss of mouse sperm motility, etc., and achieves the advantages of simple preparation method, lower average litter rate, and good application prospects Effect
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[0027] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.
[0028] 1. Preparation of CatSper1 Gene Vaccine
[0029] 1. Construction of recombinant eukaryotic vector for co-expression of CatSper1 and BAFF double genes
[0030] (1) Cloning of CatSper1 full-length cDNA
[0031] According to the CatSper1 gene sequence with the GenBank accession number NM_053054, the following primers were designed and synthesized to PCR amplify the full-length CatSper1 cDNA with restriction sites at both ends: upstream primer F1: 5'-aat ctgcag atggatcaaaactcagtgcct-3' (SEQ...
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