Gene vaccine of sperm-specific cationic channel protein CatSper1 and preparation method thereof

A technology of gene vaccine and channel protein, which is applied in the field of preparation of the vaccine, can solve the problems of reduction, loss of fertilization ability, loss of mouse sperm motility, etc., and achieves the advantages of simple preparation method, lower average litter rate, and good application prospects Effect

Inactive Publication Date: 2011-12-07
ARMY MEDICAL UNIV
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Problems solved by technology

Gene knockout experiments showed that CatSper1 gene-deficient mice had greatly reduced sperm motility, could not pass through eggs with intact zona pellucida, and lost the ability to fertilize

Method used

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  • Gene vaccine of sperm-specific cationic channel protein CatSper1 and preparation method thereof
  • Gene vaccine of sperm-specific cationic channel protein CatSper1 and preparation method thereof
  • Gene vaccine of sperm-specific cationic channel protein CatSper1 and preparation method thereof

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Embodiment Construction

[0027] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0028] 1. Preparation of CatSper1 Gene Vaccine

[0029] 1. Construction of recombinant eukaryotic vector for co-expression of CatSper1 and BAFF double genes

[0030] (1) Cloning of CatSper1 full-length cDNA

[0031] According to the CatSper1 gene sequence with the GenBank accession number NM_053054, the following primers were designed and synthesized to PCR amplify the full-length CatSper1 cDNA with restriction sites at both ends: upstream primer F1: 5'-aat ctgcag atggatcaaaactcagtgcct-3' (SEQ...

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Abstract

The invention relates to a vaccine, in particular to a gene vaccine of sperm-specific cationic channel protein CatSper1. The vaccine is an immune-stimulating complex type vaccine composed of CatSper1-BAFF digenic recombinant eukaryotic vector, saponin Quil A, cholesterol and lecithin; and the vaccine can inhibit the movement of sperms, perform specific reduction of the average farrowing rate and number born of BALB / c mouse and have good application prospect in the immunological contraception field. The invention also relates to a preparation method of the vaccine which has simple operation and low cost.

Description

technical field [0001] The invention relates to a vaccine, in particular to a gene vaccine of sperm specific cation channel protein CatSper1, and also relates to a preparation method of the vaccine. Background technique [0002] Vaccine development based on sperm antigens represents a promising avenue for contraception. The application of sperm antigens in the development of contraceptive vaccines lies in their sperm specificity, participation in human reproduction, and the ability to generate an immune response sufficient to terminate fertilization in the reproductive tract. [0003] CatSper1 is a cation channel protein unique to sperm, specifically expressed in the testis and epididymis, mainly located in the main segment of the sperm tail, and plays an important role in regulating sperm hyperactivation. Gene knockout experiments showed that the sperm motility of CatSper1 gene-deficient mice was greatly reduced, unable to pass through eggs with intact zona pellucida, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K39/00A61P15/16C12N15/12C12N15/85
Inventor 杨曌吴玉章
Owner ARMY MEDICAL UNIV
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