Gene chip and kit for detecting human papillomavirus (HPV)

Abstract

The invention discloses a gene chip for detecting human papillomavirus (HPV), comprising a solid carrier and a human papillomavirus detecting probes fixed on the solid carrier. The detecting probes comprise nucleotide sequences in SEQ ID No.9-37, and the 27 types of HVP subtype specific probes in the invention correspondingly comprise 22 kinds of high-risk and mediate-risk HPV, and 5 kinds of low-risk HPV and two kinds of high-risk HPV integration (HPV16/HPV18). The invention also discloses a detecting kit for detecting various subtypes of HPV in a sample with high speed and high flux, and the kit comprises the gene chip, a primer sequence for detection, a human GAPDH gene, special HPV lysing solution, PCR reaction liquid and a color reagent, wherein the detecting probe fixed on the gene chip has the nucleotide sequence in the SEQ ID No.9-37; the primer sequence for detection is used for amplifying the DNA sequence of the HPV in a clinical sample, and the human GAPDH gene is used for amplifying the clinical sample. The HPV detection has important significance for the clinical diagnosis of HPV as well as the early prevention, the diagnosis, the treatment and the postoperation tracking of cervical cancer.

Description

technical field [0001] The invention belongs to the field of life science and biotechnology, in particular to a gene chip containing human papillomavirus (HPV) subtype probes, and also relates to a kit for HPV detection and typing. Background technique [0002] About 510,000 women worldwide are diagnosed with cervical cancer every year, which is the second most common gynecological malignancy after breast cancer, and its incidence is now showing a younger trend. In China, about 135,000 new cases of cervical cancer are added each year, accounting for 1 / 3 of the global incidence, and about 80,000 people die from the disease. Studies have found that more than 90% of cervical cancer patients are associated with human papillomavirus (human papillomaviruses, HPV) infection. The occurrence of cervical cancer and its precancerous lesions is the result of the synergy of many factors. High-risk HPV infection is the initiating factor for the evolution of cervical cancer, and the integ...

AI Technical Summary

Problems solved by technology

However, this method can only detect whether there is HPV infection in the sample, and cannot identify the specific HPV virus subtype, which is not conducive to clinical follow-up inspection and treatment.

[0007] The integration of high-risk HPV DNA into the host genome will cause host cells to produce a series of irreversible chain reactions including functional defects of tumor suppressor genes, activation of oncogenes, genome instability and cell immortalization, which is one of the important mechanisms of cervical carcinogenesis. Its warning value is higher than that of the current general HPV-DNA infection detection, but the probes used in the current commercially available kits lack the detection of HPV integration. In addition, the existing HPV detection membrane strips are not clearly divided to detect HPV , it is easy for ordinary clinicians to misdiagnose. The present invention detects and improves the above two clinically important indicators, making the detection of clinical HPV infection richer, more accurate, more practical and more valuable

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