Short peptide for inhibiting growth of solid tumor and leukemia cancer cells and application thereof

A cancer cell, short peptide technology, applied in the field of biotechnology and medicine, can solve the problems of patient suffering, chemotherapy drugs myelosuppression, damage to patients' normal cells, etc., to achieve the effect of solving serious injuries

Inactive Publication Date: 2010-09-08
福州蓝昊生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing cancer cells, it will also damage the patient's normal cells
The most common side effect of chemotherapy drugs is the extreme suppression of the patient's bone marrow, causing great pain to the patient, and even death
So far, in addition to early detection of cancer and surgical resection of tumor masses, there is still no specific drug to treat spread tumors and leukemia [Cancer Biology, written by R.J.B Jin, translated by Liu Yixun, Science Press, 2002 ], most of the existing clinically used drugs for cancer treatment are chemical compounds, while the application of short peptides in cancer has not been reported in the literature

Method used

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  • Short peptide for inhibiting growth of solid tumor and leukemia cancer cells and application thereof
  • Short peptide for inhibiting growth of solid tumor and leukemia cancer cells and application thereof
  • Short peptide for inhibiting growth of solid tumor and leukemia cancer cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0016] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0017] The main amino acid sequence of the short peptide is a main sequence with fixed amino acid positions, and the corresponding amino acids are selected and arranged at the interval positions. For example, there are 3 kinds of amino acids at the J position: Pro, Leu or Ile; these 3 kinds of amino acids can be single Placed at the J position in , no matter which one is selected, the short peptide still has its original biological activity, that is, inhibits the growth of malignant tumor cells, and the same is true for the am...

specific Embodiment approach

[0032] Below in conjunction with embodiment, further set forth the present invention. These implementation examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0033] All synthetic short peptides involved in all the following enumerated examples have the pharmaceutical functions involved in the present invention and are not limited to the enumerated functions.

Embodiment 1

[0035] Test of inhibiting liver cancer cells (synthesis of the short peptide involved in the present invention: Pro-Gly-Val-Tyr-Thr-Lys)

[0036] The culture of human liver cancer cell line HepG2 cells was cultivated with RPMI 1640 medium containing 10% newborn bovine serum, with 10 4 The cell density per well was planted into a 96-well cell plate. After 24 hours, the short peptides involved in this experiment were added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. After 24, 48 and 72 hours, The absorbance was detected in a microplate reader by the MTT method at 570 nm. Compared with the control group, the inhibition rates of liver cancer cells were obtained. The inhibition rates of high-concentration PK-6 were 5.01%, 29.62% and 46.96%, respectively, and the inhibition rates of low-concentration PK-6 were 16.53%, 30.40% and 28.97%. . like figure 1 shown.

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Abstract

The invention provides a short peptide for inhibiting the growth of cancer cells. The main sequence of amino acid of short peptide segment, analogue, derivative and variant of the short peptide has more than 70 percent of uniformity and more than 90 percent of similarity with the main sequence of the short peptide amino acid; the short peptide, nucleotide, short peptide segment, short peptide analogue, short peptide derivative and short peptide variant are used for preparing a drug with the functions of treating leukemia caused by hyperplasia of various lesioned cells of medullary system of a patient with non-solid tumor, treating various cancers of cell proliferation of the soil tumor of the patient, diagnosing and detecting the occurrence of cancers and the grade malignancy thereof; and the short peptide or the nucleotide is used for preparing a drug or a detection reagent which can be used as a protease inhibitor, or an accelerator and or affinity reagent. The technology can solve the defects that the existing cancer drug has serious damage to normal cells of a human body while inhibiting or killing cancer cells simultaneously.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide for inhibiting the growth of cancer cells and its application. Background technique [0002] Chemotherapy has always been the main part in the process of cancer occurrence and treatment. The existing clinical chemotherapeutic drugs, such as cyclophosphamide, cisplatin, cytarabine, doxorubicin and vincristine, etc., are mainly used to destroy the cell cycle, and RNA, DNA and protein are all of them. target of drugs. In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing and killing cancer cells, it will also damage the normal cells of the patient. The most common side effect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K5/08C07K5/10C07K19/00C12N15/11A61K38/08A61K38/06A61K38/07A61K31/7088A61P35/00A61P35/02C12Q1/68G01N33/574G01N33/573
Inventor 李昊
Owner 福州蓝昊生物医药有限公司
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