Small peptide for inhibiting solid tumor and leukaemia cancer cell growth and use thereof

A technology for cancer cells and leukemia, applied in the field of biotechnology and medicine, can solve the problems of pain, death, damage to normal cells of patients, etc., and achieve the effect of solving serious injuries

Inactive Publication Date: 2009-08-05
FUJIAN LANHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing cancer cells, it will also damage the patient's normal cells
The most common side effect of chemotherapy drugs is the extreme suppression of the patient's bone marrow, causing great pain to the patient, and even death
So far, in addition to early detection of cancer and surgical resection of tumor masses, there is still no specific drug to treat spread tumors and leukemia [Cancer Biology, written by R.J.B Jin, translated by Liu Yixun, Science Press, 2002 ], most of the existing clinically used drugs for cancer treatment are chemical compounds, while the application of short peptides in cancer has not been reported in the literature

Method used

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  • Small peptide for inhibiting solid tumor and leukaemia cancer cell growth and use thereof
  • Small peptide for inhibiting solid tumor and leukaemia cancer cell growth and use thereof
  • Small peptide for inhibiting solid tumor and leukaemia cancer cell growth and use thereof

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Experimental program
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preparation example Construction

[0018] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0019] The main amino acid sequence of the short peptide is a main sequence with fixed amino acid positions, and the corresponding amino acids are selected and arranged at the interval positions. For example, there are three kinds of amino acids at the J position: Pro, Asn or Ala; these three kinds of amino acids can be single Placed at the J position in , no matter which one is selected, the short peptide still has its original biological activity, that is, inhibits the growth of malignant tumor cells, and the same is true fo...

Embodiment 1

[0036] Test of inhibiting liver cancer cells (synthesis of the short peptide involved in the present invention: Pro-Ala-Leu-Asn-Thr-Val-Lys)

[0037] The human liver cancer cell line Hepg2 cells were cultured with RPMI 1640 medium containing 10% newborn bovine serum, with 5×10 5 The cell density per well was planted into a 96-well cell plate. After 24 hours, the short peptides involved in the present invention were added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. After 24, 48, and 72 hours, The survival rate of living cells was tested by MTT method, and the inhibition rate of liver cancer cells was obtained by comparing the administration group with the control group. Such as figure 1 shown.

Embodiment 2

[0039] Inhibition of acute myeloid leukemia (M 2 ) (synthesis of the short peptide derivatives involved in the present invention, modified by adding phosphoric acid group at serine: Pro-Ala-Serp-Asn-Thr-Val-Lys)

[0040] Human acute myeloid leukemia cell line HL60 cells were cultured with RPMI 1640 medium containing 10% newborn bovine serum, and cultured in 10 6 After the cell density per well is planted into a 24-well cell plate, the short peptides involved in the present invention are immediately added to the cell culture plate according to different concentrations, and an equal volume of PBS is used as a control group. The experimental results show that the cells in the 5 days The inhibition rate reached 80%.

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Abstract

The invention provides a short peptide for inhibiting the solid tumor and the growth of the cancer cells of leukemia. The amino acid main sequence of the short peptide fragment, the analogue, the derivative and the variant of the short peptide has the sameness being equal to or more than 70 percent of the amino acid main sequence of the short peptide and has the similarity being equal to or more than 90 percent of the amino acid main sequence of the short peptide; and the short peptide, the nucleotide, the short peptide fragment, the short peptide analogue, the short peptide derivative and the short peptide variant are used for preparing drugs for curing the leukemia caused by hyperplasia of various pthological cells of the non-solid tumor human myeloblast, curing various cancers of cancer cell multiplication of the human solid tumor and diagnosing and detecting the occurrence of the cancer as well as the grade malignancy of occurrence of the cancer, and the short peptide or the nucleotide is used for preparing the drugs as the protease inhibitor, the promoter or the nucleophilic reagent or detecting the detection of the application of the reagent. The technology can solve the problem that the prior cancer drug has serious harm to the normal cells of the human body when inhibiting or killing the cancer cells.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a short peptide for inhibiting the growth of solid tumor and leukemia cancer cells and its application. Background technique [0002] Chemotherapy has always been the main part in the process of cancer occurrence and treatment. The existing clinical chemotherapeutic drugs, such as cyclophosphamide, cisplatin, cytarabine, doxorubicin and vincristine, etc., are mainly used to destroy the cell cycle, and RNA, DNA and protein are all of them. target of drugs. In recent years, As 2 o 3 Great progress has been made in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity. Whether one chemotherapy drug is used alone or several chemotherapy drugs are used in clinical treatment, It has obvious curative effect on cancer, while killing and killing cancer cells, it will also damage the normal cells of the patient. The...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N15/11C12Q1/68A61K38/08A61K31/7088A61K48/00A61P35/02A61P35/00G01N33/574
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
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