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Mi RNA marker related to side effect of platinum-based chemotherapy to advanced NSCLC patient and application thereof

A non-small cell lung cancer, toxic and side effect technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of easy-to-obtain materials, convenient and flexible operation, and less restrictions

Inactive Publication Date: 2010-09-08
EAST CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the correlation between hsa-mir-196a2 rs11614913 and the side effects of non-small cell lung cancer platinum chemotherapy has not been reported at home and abroad, that is, the application of this single base polymorphism site in the personalized treatment of lung cancer still needs further research. Research

Method used

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  • Mi RNA marker related to side effect of platinum-based chemotherapy to advanced NSCLC patient and application thereof
  • Mi RNA marker related to side effect of platinum-based chemotherapy to advanced NSCLC patient and application thereof
  • Mi RNA marker related to side effect of platinum-based chemotherapy to advanced NSCLC patient and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of a deoxyribonucleic acid kit for detecting or predicting the degree of toxic and side effects of platinum-based chemotherapy in patients with advanced non-small cell lung cancer

[0019] The production and operation process of the deoxyribonucleic acid kit uses the commonly used deoxyribonucleic acid sequencing technology and amplification technology.

[0020] The kit can only include DNA primers of hsa-mir-196a2 rs11614913 genotype, or it can also be equipped with common reagents required for DNA amplification. The user needs to prepare the genomic DNA extracted from the normal tissue cells of the patient, and then select the amplification method and sequencing method according to the specific experimental conditions to obtain the genotype of the patient's hsa-mir-196a2 rs11614913. This kit has few operating restrictions and flexible methods. It is very simple and easy to predict the degree of toxic and side effects of platinum-based chemotherap...

Embodiment 2

[0021] Example 2 Collecting samples and extracting DNA

[0022] I. Screen the research population according to uniform standards and establish a database

[0023] Patients must be diagnosed with stage III or IV non-small cell lung cancer in the TNM staging system, inoperable, aged between 18 and 70, without a history of malignant tumors, and have not undergone surgery or chemotherapy before. In this study, 445 eligible patients were recruited. They provided various clinical information systematically and truthfully through questionnaires and other methods. We established a database on this basis.

[0024] II. Collect blood samples from patients and perform genotyping

[0025] Blood samples are collected from patients before they start chemotherapy. We extracted genomic deoxyribonucleic acid from peripheral blood leukocytes, designed primers and carried out polymerase chain reaction (PCR) amplification, and successfully analyzed the gene of hsa-mir-196a2 rs11614913 in 442 pat...

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Abstract

The invention relates to a mi RNA marker related to side effects of platinum-based chemotherapy to advanced non-small cell lung cancer (NSCLC) patients and application and related kit thereof. The marker is hsa-mir-196ars11614913 and can be used in detection or prognoses of the side effects of platinum-based chemotherapy to the advanced NSCLC patients, thereby the marker can provide a basis for the individualistic drug therapy to the advanced NSCLC patients.

Description

technical field [0001] The present invention relates to medical genetics and clinical medicine, specifically a miRNA marker related to the toxic and side effects of platinum-based chemotherapy in patients with advanced non-small cell lung cancer and its application. Different genotypes of specific single-base polymorphism sites are applied to Predict the severity of side effects of platinum-based chemotherapy in patients with advanced non-small cell lung cancer, and provide medication basis for personalized treatment of such patients. Background technique [0002] Lung cancer has always been a cancer with the highest mortality rate in the world, and non-small cell lung cancer (NSCLC) is the cause of death in 85% of patients. Because lung cancer is generally difficult to detect, patients are often diagnosed at an advanced stage. Therefore, it is very important for the clinical treatment of advanced non-small cell lung cancer. [0003] MicroRNA (miRNA) is a class of endogeno...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 吴奇涵詹晓莹
Owner EAST CHINA NORMAL UNIVERSITY
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