The invention discloses an in-situ gelling chemotherapy immune pharmaceutical composition, which comprises a first composition, a second composition and a third composition, the first composition comprises a fat-soluble immunologic adjuvant and a surfactant, the second composition comprises a carboxylic acid ligand-containing platinum chemotherapy drug capable of causing immunogenic death and a protective filler, and the third composition comprises a carboxylic acid ligand-containing platinum chemotherapy drug capable of causing immunogenic death and a carboxylic acid ligand-containing platinum chemotherapy drug capable of causing immunogenic death and a carboxylic acid ligand-containing platinum chemotherapy drug capable of causing immunogenic death. The third composition is prepared from soluble alginate, a protective filling agent and a pH (Potential of Hydrogen) regulator. The invention provides a composition with better stability, which can better generate a synergistic anticancer effect, reduce side effects, reduce the metastasis probability and recurrence probability of cancers, and inhibit the growth of distal metastatic tumors and reduce the recurrence probability of tumors through immunoreaction while effectively killing in-situ tumors. The invention further discloses a preparation method of the in-situ gelling chemotherapy immune composition, and the product system is good in stability and easy to store for a long time.