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Tissue culture method and special culture medium of Spanish dagger anther

A culture medium and technology of Pteris orchids, applied in the field of plant tissue culture and crop breeding, to achieve the effect of improving selection efficiency

Inactive Publication Date: 2012-01-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieving domestic and foreign materials, there is no report on the cultivation of anthers and green seedling regeneration of Pteris orchid

Method used

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  • Tissue culture method and special culture medium of Spanish dagger anther
  • Tissue culture method and special culture medium of Spanish dagger anther
  • Tissue culture method and special culture medium of Spanish dagger anther

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Preparation and statistical observation of haploid plants

[0030] 1. Obtainment and statistical observation of callus induced by anthers

[0031] 1. Pretreatment of anthers

[0032] Collect the flower buds of Phnom Penh Phoenix orchid (purchased from Beijing Xiaoying Flower Market) in the single-core stage, first rinse with tap water 3-5 times, dark treatment at 4 ℃ low temperature for 3 days, and then use 75% (volume percentage ) Alcohol sterilization for 40 seconds, rinse with sterile water 5 times, then sterilize with 0.1% (mass percentage) mercury liters for 6 minutes, and rinse 5 times with sterile water.

[0033] 2. Anther-induced callus acquisition

[0034] Use tweezers to carefully remove the anthers (microspores of anthers in the mononuclear side stage, such as figure 1 (Shown) inoculated on callus induction medium, and cultured in the dark for one week at 25°C, and then cultivated at a temperature of 23°C, an illumination intensity of 2000 Lx, and an illumi...

Embodiment 2

[0055] Example 2. Plant ploidy doubled

[0056] The 2 haploid Phnom Penh chinensis plants that were detected as haploid obtained in Example 1 were treated with 0.1% colchicine (mass percentage) for 24 hours to obtain homozygous diploid plants.

[0057] The treatment method is: first filter and sterilize 0.1% colchicine, then dip a sterile cotton ball into an appropriate amount of the above-mentioned colchicine solution, and then place the small cotton ball on the growth point of the plant for 24 hours.

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Abstract

The invention discloses a tissue culture method and a special culture medium of a Spanish dagger anther. A callus induction culture medium of Spanish dagger is a solid culture medium obtained by adding the following matters of 6-BA with final concentration of 4.5-5.5mg / L, KT with final concentration of 0.2-0.4mg / L, NAA with final concentration of 0.4-0.6mg / L, a carbon source and jellies into MS basic culture fluid. The tissue culture method comprises the following steps of: culturing the Spanish dagger anther on a special callus induced medium to obtain callus, and grafting the Spanish daggeranther on a differential medium to carry out differentiation culture to obtain a regeneration plant. The callus induced medium can effectively induce the Spanish dagger anther to differentiate the callus, and the inductivity of the callus of the Spanish dagger anther can reach over 22.7 percent. The tissue culture method can provide a basis for haploid breeding.

Description

Technical field [0001] The present invention relates to the fields of plant tissue culture and crop breeding, in particular to a method for tissue culture of Phalaenopsis orchid and its special culture medium. Background technique [0002] Phoenix orchid, also known as pineapple flower, is an evergreen shrub of the lily family Yucca. It is native to North America and has high ornamental value. It is designated as the national flower by Seychelles. It is widely cultivated throughout the Yangtze River valley in China. It is an ideal flower and tree for garden greening. It has high environmental value, has strong resistance to sulfur dioxide, chlorine, etc., and can absorb harmful gases. It is a good green product for industrial and mining polluted areas (noble, 2005). [0003] Pang Yi et al. (Pang Yi, et al. Tissue culture of Phoenix orchid[J]. Tropical Agricultural Sciences, 2006, 26(5):24-27.) The stem tip and tenderness of Yucca gloriosa Linnaeus The stems and young leaves are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 郭仰东赵永钦刘莉莎吴新新郑禾
Owner CHINA AGRI UNIV