Common wheat low molecular weight glutenin as well as encoding gene and application thereof

A low molecular weight, glutenin technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of difficult to find subunits, and achieve the effect of improving weak gluten

Inactive Publication Date: 2017-07-25
INNER MONGOLIA AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weights of the subunits of different alleles in the three sites are very close, and the identification of some subunits needs to be distinguished based on their concentration, so it is difficult to find new subunits

Method used

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  • Common wheat low molecular weight glutenin as well as encoding gene and application thereof
  • Common wheat low molecular weight glutenin as well as encoding gene and application thereof
  • Common wheat low molecular weight glutenin as well as encoding gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0038] A common wheat low-molecular-weight glutenin coding gene named GluD3-13 has a nucleotide sequence as shown in SEQ ID No.:2.

[0039] 1. Discovery of allelic variation GluD3-13

[0040] A total of 10 wheats of strong gluten type, medium gluten type and weak gluten type wheat were used as test materials, and its LMW-GS gene was carried out on chromosome 1A with sequence 5 (SEQ ID №: 5) and sequence 6 (SEQ ID No. ID №: 6) primers 1B2F / LB2R for PCR amplification, and then sequencing, PCR product sequencing adopts two methods, one is direct sequencing, and the other is cloning (using the pEASY-T1 carrier of Beijing Quanshijin Biotechnology Co., Ltd. ) followed by sequencing, and each sample was sequenced 3-6 times (sequencing was completed by Nanjing GenScript Biotechnology Co., Ltd.). The present invention respectively uses the above two methods to sequence the PCR products, and the sequencing results obtained by the two methods are consistent.

[0041] The experimental r...

Embodiment 2

[0050] A common wheat low molecular weight glutenin named P-13 (SEQ ID №: 1), the amino acid sequence is shown in SEQ ID №: 1. Produced by the GluD3-13 (SEQ ID №: 2) coding gene: extract the Yanzhan 4110 genomic DNA by the CTAB method, use the Yanzhan 4110 genomic DNA as a template, and use the sequence 5 (SEQ ID №: 5) and the sequence in the sequence table The primer LB2F / LB2R composed of 6 (SEQ ID No.: 6) was amplified by PCR to obtain the GluD3-13 nucleotide sequence, and the coding region sequence of the nucleotide sequence could be obtained by using DNAMAN.6.0.3.99 Chinese version software.

[0051] A typical LMW-GS polypeptide chain structure mainly includes four structural regions, respectively, the initial sequence is a highly conserved signal peptide including 20 amino acids, followed by the N-terminal conservative region, N-terminal repeat region and C- Terminal conserved region (divided into 3 sub-regions namely cysteine-rich region, glutamine-rich region and termin...

Embodiment 3

[0055] Application of a common wheat low molecular weight glutenin coding gene in wheat crop breeding.

[0056] The variety of wheat used is Yanzhan 4110. LMW-GS containing 7 Cys residues was obtained from common wheat Yanzhan 4110, and Yanzhan 4110 can be used as a parent for breeding by means of hybridization technology. With the further development of technology and methods, new breakthroughs have been made in the discovery of new LMW-GS genes, the relationship between each allele and wheat quality, etc., so that LMW-GS can be well applied in wheat breeding, and finally achieve high-quality purpose of wheat.

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Abstract

The invention discloses common wheat low molecular weight glutenin as well as an encoding gene and application thereof. An amino acid sequence of the common wheat low molecular weight glutenin is as shown in SEQ ID No. 1; a nucleotide sequence of the encoding gene of the common wheat low molecular weight glutenin is as shown in SEQ ID No. 2; the invention also discloses a method for identifying whether common wheat contains the encoding gene as shown in the SEQ ID No. 2 or not. Allele variant type GluD3-13 is found on the 1A chromosome of Yanzhan 4110 for the first time, so that an excellent exogenous gene can be provided for wheat breeding. A peptide chain P-13 corresponding to the allele variant type GluD3-13 and LMW-GS containing seven cysteine residues can reduce the protein of flour and improve the weak gluten property of flour quality, and can be used as good parents for hybridization of high-quality wheat materials. The Yanzhan 4110 is a gene resource for high quality wheat breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a common wheat low-molecular-weight glutenin, its coding gene and its application. Background technique [0002] Wheat belongs to the genus Triticum genus of Gramineae, an annual or perennial herbaceous plant, which is the general name of wheat plants; wheat has strong adaptability, wide distribution and many uses, and is one of the important food crops in the world. Its total area, total output and total The trade volume ranks first among food crops. In recent years, more and more attention has been paid to the improvement of wheat quality, and the content and quality of protein play a decisive role in the quality of wheat processing. According to the difference of wheat gluten, wheat can be roughly divided into strong gluten type, medium gluten type and weak gluten type wheat; strong gluten and weak gluten wheat are generally called high-quality wheat. Wheat with different gluten...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12Q1/68C12N15/82C12N5/10A01H5/00
CPCC07K14/415C12N15/8251C12Q1/6869C12Q2531/113
Inventor 杨燕董雪赵献林冯玉梅刘梦赵永英王增
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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