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Tissue culture method and special culture medium of Spanish dagger anther

A culture medium and a technique for anchovy orchid, which are applied in the field of plant tissue culture and crop breeding to achieve the effect of improving selection efficiency

Inactive Publication Date: 2010-10-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieving domestic and foreign materials, there is no report on the cultivation of anthers and green seedling regeneration of Pteris orchid

Method used

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  • Tissue culture method and special culture medium of Spanish dagger anther
  • Tissue culture method and special culture medium of Spanish dagger anther
  • Tissue culture method and special culture medium of Spanish dagger anther

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, preparation and statistical observation of Pteris orchid haploid plant

[0030] 1. The acquisition of anther-induced callus and its statistical observation

[0031] 1. Pretreatment of anthers

[0032] Collect the flower buds of Phnom Penh orchids (purchased from Beijing Xiaoying Flower Market) that are in the single-nucleus stage, rinse them with tap water 3-5 times, and treat them in the dark at a low temperature of 4°C for 3 days, then use 75% (volume percentage) ) alcohol disinfection for 40 seconds, rinsed with sterile water for 5 times, then sterilized with 0.1% (mass percentage) mercuric chloride for 6 minutes, and rinsed with sterile water for 5 times.

[0033] 2. Acquisition of callus induced by anthers

[0034] The flower bud after the pretreatment in the above-mentioned steps 1 is carefully anther (the anther microspore of uninucleate marginal stage such as figure 1 Shown) were inoculated on the callus induction medium, and under the condition...

Embodiment 2

[0055] Embodiment 2, plant ploidy doubling

[0056] Homozygous diploid plants can be obtained by treating the two haploid Phnom Penh orchid plants detected as haploid obtained in Example 1 above with a concentration of 0.1% colchicine (mass percentage) for 24 hours.

[0057] The treatment method is as follows: firstly filter and sterilize 0.1% colchicine, then use a sterile cotton ball to dip an appropriate amount of the above-mentioned colchicine solution, then place the small cotton ball on the growth point of the plant, and treat for 24 hours.

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Abstract

The invention discloses a tissue culture method and a special culture medium of a Spanish dagger anther. A callus induction culture medium of Spanish dagger is a solid culture medium obtained by adding the following matters of 6-BA with final concentration of 4.5-5.5mg / L, KT with final concentration of 0.2-0.4mg / L, NAA with final concentration of 0.4-0.6mg / L, a carbon source and jellies into MS basic culture fluid. The tissue culture method comprises the following steps of: culturing the Spanish dagger anther on a special callus induced medium to obtain callus, and grafting the Spanish dagger anther on a differential medium to carry out differentiation culture to obtain a regeneration plant. The callus induced medium can effectively induce the Spanish dagger anther to differentiate the callus, and the inductivity of the callus of the Spanish dagger anther can reach over 22.7 percent. The tissue culture method can provide a basis for haploid breeding.

Description

technical field [0001] The invention relates to the fields of plant tissue culture and crop breeding, in particular to a method for tissue culture of anther orchid anthers and a special culture medium thereof. Background technique [0002] Pineapple orchid, also known as pineapple flower, is an evergreen shrub of the genus Yucca in the family Liliaceae. It is native to North America and has high ornamental value. It is designated as the national flower of Seychelles. It is widely cultivated throughout the Yangtze River Basin in China. It is an ideal flower and tree for garden greening, and has high environmental protection value. It has strong resistance to sulfur dioxide, chlorine, etc., and can absorb harmful gases. It is a good greening product in industrial and mining polluted areas (noble, 2005). [0003] Pang Yi et al. (Pang Yi, et al. Tissue culture of Yucca gloriosa Linnaeus [J]. Tropical Agricultural Science, 2006, 26 (5): 24-27. Stems and young leaves were used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郭仰东赵永钦吴新新
Owner CHINA AGRI UNIV