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Method for enriching urine protein directly

A urine protein, direct technology, applied in the field of urine protein enrichment, can solve the problems of urine protein degradation, severe denaturation, strong odor, and not meeting urban sanitation requirements, etc.

Inactive Publication Date: 2010-10-20
GUANGDONG TECHPOOL BIO-PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method collects male urine, which has a strong smell and does not meet the urban hygiene requirements. The urine required for the production of crude urine protein has been completely withdrawn from large and medium-sized cities. At present, the collected urine mainly comes from small towns and villages. The cost is getting higher and higher, and the collection time interval is getting longer and longer. The degradation and denaturation of urine protein is more serious, which brings more difficulties to the subsequent purification.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 10 kilograms of macroporous anion exchange resin (polystyrene resin, ligand is [-N + (CH 3 ) 3 〕), after regeneration treatment with 3% hydrochloric acid and 3% NaOH, flush the water to neutrality, put it into a filter cloth bag with a pore size of 50 mesh, and place the filter cloth bag filled with resin in the toilet or urinal of the toilet (can be used every day) Put 1 kg of resin in the urinal that flows through 80L of urine), urine or urine diluted with water flows through the resin; after 24 hours, concentrate the resin that absorbs urine protein to the processing point, and use 10% ammonium sulfate solution for treatment. Elution: add ammonium sulfate powder to the eluent to saturation, let it stand for 2 hours, filter the precipitated part through diatomaceous earth, dry the precipitate, and obtain 120 grams of urinary trypsin inhibitor and human urinary kininogenase crude product. Each gram of crude product contains 31000 U of urinary trypsin inhibitor and 0....

Embodiment 2

[0042] Change the ligand to -N + (CH 3 ) 3 Each 1 kilogram of polystyrene resin, acrylic resin, phenolic resin, dextran, chitosan, cellulose adsorbent, regenerate respectively, after equilibrium treatment, carry out urine protein adsorption and wash-out by the method for embodiment 1, the result : Ligand is -N + (CH 3 ) 3 The polystyrene resin and acrylic resin obtained 10 grams and 9.5 grams of crude products respectively, the total activity of urinary trypsin inhibitors were 325000U and 287000U respectively, containing human urinary kininogenase 3.2PNA, 2.6PNA, other adsorbent urinary trypsin The total activity of the inhibitors was lower than 200000U, and the human urinary kallikreinase was lower than 2PNA.

Embodiment 3

[0044] 20 kilograms of macroporous cation exchange resin (acrylic resin, ligand is -SO 3 - ), after regeneration treatment with 3% hydrochloric acid and 3% NaOH, flush the water to neutral, put it into a filter cloth bag with a pore size of 50 mesh, and place the filter cloth bag filled with resin in the toilet or urinal of the toilet (it can flow every day) Put 1 kg of resin in the urinal with 30L of urine), urine or urine diluted with water flows through the resin; after 24 hours, concentrate the resin that absorbs urine protein to the processing point, and wash it with 10% ammonium sulfate solution Add ammonium sulfate powder to the eluent to saturation, let it stand for 2 hours, filter the precipitated part through diatomaceous earth, and dry the precipitate to obtain 50 grams of crude urokinase. Each gram of crude product contains 36000U of urokinase.

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PUM

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Abstract

The invention relates to a urine protein enriching method. In the method, urine protein in urine is absorbed directly by an adsorbent in a urinal or a pee hopper, and the adsorbent for adsorbing the urine protein is conveyed to a working point for follow-up treatment; and based on the isoelectric point properties of specific urine protein, the urine protein such as urinary trypsin inhibitor, human urinary kininogenase, urokinase and the like is adsorbed directly and effectively by using the specific adsorbent such as ion exchange resin, and thus, the step of collecting the urine is avoided. The method does not influence sanitation of toilets obviously, reduces the cost of urine transportation substantially and solves a series of environmental problems.

Description

technical field [0001] The invention relates to a method for enriching urine protein by directly using an adsorbent in a urinal or a urinal to absorb urine protein in urine, and transporting the adsorbent for absorbing urine protein to a processing point for subsequent treatment. Background technique [0002] Urinary trypsin inhibitor, also known as human urinary trypsin inhibitor (Human Urinary Trypsin Inhibitor). Urinary trypsin inhibitor is a glycoprotein composed of 143 amino acids, its molecular weight is 60-70KD as determined by HPLC, 40-50KD as determined by SDS-PAGE, and its isoelectric point is 2.6. It is a heat-stable Acid protein. The protein is currently mainly used in the treatment of pancreatitis, shock and other diseases. [0003] Urokinase (Urokinase, abbreviated as UK) is an alkaline protease produced by human renal tubular epithelial cells and secreted into urine. / ml. Among natural sources of urokinase, there are mainly two forms of urokinase molecules...

Claims

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Application Information

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IPC IPC(8): C07K14/81C07K1/22C12N9/72C12N9/64
Inventor 傅和亮苗丕渠郑少亮王晓岩田友军许文勤
Owner GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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