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Method for preparing microbe-derived bio-pesticide

A technology of microbial pesticides and biological sources, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve problems such as ecological imbalance, environmental pollution and teratogenicity of chemical pesticides, and achieve ecological benefits The effect of protection and convenience

Inactive Publication Date: 2010-11-24
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the annual production of pesticides at home and abroad is more than 2 million tons, and chemical pesticides occupy an absolute dominant position. However, the adverse consequences of chemical pesticides causing environmental pollution, ecological imbalance, teratogenicity, carcinogenicity, poisoning and death are becoming more and more prominent.

Method used

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  • Method for preparing microbe-derived bio-pesticide
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  • Method for preparing microbe-derived bio-pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: the production technology of microbial pesticide of the present invention

[0052] 1. Culture medium

[0053] 1. Inclined culture medium:

[0054] Glucose 10g / L, potato (peeled) 200g / L, agar 15g / L, prepared with distilled water, pH7.2-7.4, 121°C for 30min.

[0055] Preparation of potato dipping juice: Take 200g of peeled potatoes, cut into small pieces, boil in 1000ml of water for an hour, filter, and fill the filtrate with cold boiled water to 1000ml.

[0056] 2. Shake flask seed medium

[0057] Glucose 20g / L, peanut meal 3.0g / L, NH 4 Cl 3.0g / L,

[0058] K H 2 PO 4 3.0g / L, MgSO 4 7H 2 O 3.0g / L, CaCO 3 4.0g / L,

[0059] NaAC·3H 2 O 1.0g / L, normal water configuration.

[0060] PH 6.0 before sterilization, 121°C for 30min.

[0061] 3. Seed tank culture medium

[0062] Glucose 20g / L, peanut meal 3.0g / L, NH 4 Cl 3.0g / L,

[0063] K H 2 PO 4 3.0g / L, MgSO 4 7H 2 O 3.0g / L, CaCO 3 4.0g / L,

[0064] NaAC·3H 2 O 1.0g / L, normal water ...

Embodiment 2

[0080] The investigation of the minimum inhibitory concentration of product of the present invention (fermentation liquid) of embodiment 2

[0081] The product of the present invention uses the tube-and-dish method to carry out bacteriostasis tests on many agricultural pathogenic fungi on the PDA medium, and the results show that the bacteriostasis effect is remarkable, as shown in Table 1. Therefore, the product of the present invention has a good broad-spectrum bactericidal effect.

[0082] The antibacterial spectrum of table 1 product of the present invention

[0083] pathogenic bacteria

[0084] cucumber wilt

Embodiment 3

[0085] Embodiment 3 The investigation of product of the present invention and chemical pesticide control effect

[0086] Comparison of the effects of the product of the present invention and chemical pesticides in treating powdery mildew, tomato late blight, capsicum late blight, cabbage wilt, small spot of corn, and large spot of corn

[0087] Table 2 product of the present invention and chemical pesticide treatment effect comparison

[0088]

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Abstract

The invention belongs to the technical field of medicaments and relates to a method for preparing microbe-derived bio-pesticide through a strain of screened fungus. The fungus is Fusariumaverenaceum (Cordaex Fr.) Sacc; the preservation date is 11 May, 2010; the preservation unit is China General Microbiological Culture Collection Center (CGMCC); and the preservation number is CGMCCNo.3823. The microbial pesticide is produced by the process flow in the invention through a seed culture medium and a fermentation medium, and the prepared pesticide has broad-spectrum functions of preventing and treating crop diseases, and has special effect on tomato late blight disease, hot pepper late blight disease, and cabbage blight. The method for preparing the bio-pesticide has the advantages of stable product quality, high efficiency, no toxin, safety for a human body, no pollution, ecological environment protection, capacity of replacing chemical pesticide for agricultural production, and capacity of meeting the development requirement on 'green agriculture'.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and relates to a method for preparing biological pesticides derived from microorganisms, in particular to a method for strain screening of microbial pesticides, bacterial liquids and preparations thereof. Background technique [0002] Crop diseases and insect pests are one of the main reasons for the reduction of crop production. Severe diseases and insect pests can lead to a large reduction in production, or even no harvest. According to the investigation of the Food and Agriculture Organization of the United Nations, 20% to 40% of the grain harvests are taken away by diseases and insect pests in the world every year, and the resulting economic losses amount to 120 billion US dollars. Therefore, pesticides play an important role in the agricultural production process. At present, the annual production of pesticides at home and abroad is more than 2 million tons, and chemical pesticides occu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C12N1/14C12R1/77
Inventor 张怡轩王勇吴春福
Owner SHENYANG PHARMA UNIVERSITY
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